Wistar Ratlarından Scopulariopsis brevicularis izolasyonu

Öz

Scopulariopsis brevicaulis is a saprophytic fungus that has wide geographic distribution. This study describes a case of hair loss and skin lesions observed in male and female Wistar rats in Turkey due to Scopulariopsis brevicaulis infection. Skin scrapings and hair samples from three male and two female albino Wistar rats were provided by the Experimental Animal Production and Experimental Research Center of Mehmet Akif Ersoy University to the Faculty of Veterinary Medicine, Department of Microbiology laboratory in Burdur for analysis in July 2019. Microbiological methods were used for species identification and Scopulariopsis brevicaulis was isolated from all of the samples. The rats completely recovered without treatment and had no recurrence of clinical signs at 1 month post-sampling. To the best of our knowledge, this is the first report of S. brevicaulis causing an infection in Wistar rats in Turkey.

Anahtar Kelimeler

• Laboratory animals
• mycological examination
• saprophyte
• Scopulariopsis sp.

Isolation of Scopulariopsis brevicaulis from Wistar Rats

Öz

Scopulariopsis brevicaulis is a saprophytic fungus that has wide geographic distribution. This study describes a case of hair loss and skin lesions observed in male and female Wistar rats due to Scopulariopsis brevicaulis infection in Turkey. Skin scrapings and hair samples from three male and two female rats were provided by the Experimental Animal Production and Experimental Research Center of Mehmet Akif Ersoy University to the Faculty of Veterinary Medicine, Department of Microbiology Laboratory in Burdur for analysis in July 2019. Microbiological methods were used for species identification and Scopulariopsis brevicaulis was isolated from all of the samples. The rats completely recovered without treatment and had no recurrence of clinical signs at one month post-sampling. This study is the first report of S. brevicaulis causing an infection in Wistar rats in Turkey. 

Anahtar Kelimeler

• : Laboratory animals
• mycological examination
• Scopulariopsis sp

Kaynakça

1. Abbott SP, Sigler L, Currah RS. (1998). Microascus brevicaulis sp. nov., the teleomorph of Scopulariopsis brevicaulis, supports placement of Scopulariopsis with the Microascaceae. Mycologia. 90(2), 297-302. doi: 10.1080/00275514.1998.12026910
2. Anandan V, Nayak V, Sundaram S, Srikanth P.(2008). An association of Alternaria alternata and Scopulariopsis brevicaulis in cutaneous phaeohyphomycosis. Indian J. Dermatol Venereol Leprol. 74(3), 244-247. doi: 10.4103/0378-6323.41371
3. Aguilar C, Pujol I, Guarro J. (1999). In vitro antifungal susceptibilities of Scopulariopsis isolates. Antimicrob Agents Chemother. 43(6), 1520-1522. 

4. Bonifaz A, Cruz-Aguilar P, Ponce RM. (2007). Onychomycosis by molds. Report of 78 cases. Eur J Dermatol. 17(1):70 -72. doi: 10.1684/ejd.2007.0092.
5. Cawcutt K, Baddour LM, Burgess M. (2015). A case of Scopulariopsis brevicaulis endocarditis with mycotic aneurysm in an immunocompetent host. Case Rep Med. 2015:871-872. doi.org/10.1155/2015/872871
6. Costa FVA, Spanamberg A, Araujo R, Werner J, Ferreiro L. (2019). Feline sino-orbital fungal infection caused by Aspergillus and Scopulariopsis. Acta Sci Vet. 47(Suppl 1), 383-341. doi: 10.22456/1679-9216.91581
7. Couto MS, Pantoja LDM, Mourao CL, Paixao GC. (2014). Fungal microbiota of the hair coat of laboratory animals. Rev Bras Vet. 17(1): 52-54.doi: 10.4322/rbcv.2014.143
8. Cuenca-Estrella M, Gomez-Lopez A, Buitrago MJ, Mellado E, Garcia-Effron G, Rodriguez- Tudela JL. (2006). In vitro activities of 10 combinations of antifungal agents against the multiresistant pathogen Scopulariopsis brevicaulis. Antimicrob Agents Chemother. 50:2248-2250. doi: 10.1128/AAC.00162-06.

9. Hampson ECGM, Gibson JS, Barot M, Shapter FM, Greer RM. (2019). Identification of bacteria and fungi sampled from the conjunctival surface of normal horses in South- East Queensland, Australia. Vet Ophthalmol. 22(3), 265-275. doi: 10.1111/vop.12587
10. Issakainen J, Heikkilä H, Vainio E, Koukila-Kähkölä P, Castren M, Liimatainen O, Ojanen T, Koskela M, Meurman O. (2007). Occurrence of Scopulariopsis and Scedosporium in nails and keratinous skin. A 5-year retrospective multi-center study. Med Mycol. 45(3):201–209. doi: 10.1080/13693780601103080.
11. Iwen P, Schutte SD, Florescu DF, Noel-Hurst RK, Sigler L. (2012). Invasive Scopulariopsis brevicaulis infection in an immunocompromised patient and review of prior cases caused by Scopulariopsis and Microascus species. J Mycol Med. 50(6), 561-569.
doi: 10.3109/13693786.2012.675629.
12. Kordalewska M, Jagielski T, Brillowska-Dabrowska A. (2016). Rapid assays for specific detection of fungi
of Scopulariopsis and microascus genera and Scopulariopsis brevicaulis species. Mycopathologia. 181(7-8), 465-474. doi: 10.1007/s11046-016- 0008-5
13. Larone DH. (1995). Medically important fungi: A guide to identification, 4rd Edition, American Society for Microbiology, USA. p. 272-274.
14. Mantovani A, Morganti L, Batelli G, Mantovani A, Poqlayen G, Tampieri MP, Vecchi G.(1982). The role of wild animals in the ecology of dermatophytes and related fungi. Folia Parasitol. 29(3): 279-284.
15. Miossec C, Morio F, Lepoivre T, Le Pape P, Garcia-Hermoso D, Gay-Andrieu F, Haloun A, Treilhaud M, Leclair F, Miegeville M. (2011). Fatal invasive infection with fungemia due to Microascus cirrosus after heart and lung transplantation in a patient with cystic fibrosis. J Clin Microbiol. 49(7):2743-2747. doi: 10.1128/JCM.00127-11
16. Nicklas W. (2004). Infectious in laboratory animals: Importance and control. Kaliste E. (Eds.) The Welfare of Laboratory Animals. Kluwer Academic Publishers, Dordrecht, Netherland. p. 153.
17. Ozturk D, Adanır R, Turutoglu H. (2009). Superficial skin infection
with Scopulariopsis brevicaulis in two goats. a case report. Bull Vet Inst Pulawy. 53, 361-363.
18. Park J-H, Seok S, Baek M, Lee H, Kim D, Cho J, Kim C, Hwang D. (2006). Microbiological monitoring of guinea pigs reared conventionally at two breeding facilities in Korea. Experimental Animal. 55(5), 427-432. doi:10.1538/expanim.55.427
19. Ragge N, Hart J, Easty D, Tyers A. (1990). A case of fungal keratitis caused by Scopulariopsis brevicaulis treatment with antifungal agent and penetrating keratoplasty. Br J Opthalmol. 74(9): 561-562. doi: 10.1128/jcm.00127-11.
20. Romano C, Gianni C, Difonzo EM. (2005). Retrospective study of onychomycosis in Italy: 1985-2000. Mycoses. 48: 42-44. doi: 10.1111/j.1439-0507.2004.01066.x
21. Samson RA, Houbraken J, Thrane U, Frisvad JC. Food and indoor fungi.(2010). CBS Laboratory Manual Series 2. CBS-Fungal Biodiversity Centre, Utrecht.
22. Sandoval-Denis M, Sutton DA, Fothergill AW, Cano-Lira J, Gené J, Decock CA, de Hoog GS, Guarro J. (2013). Scopulariopsis, a poorly known opportunistic fungus: spectrum of species in clinical samples and in vitro responses to antifungal drugs. J Clin Microbiol. 51(12):3937-3943. doi: 10.1128/JM.01927-13.
23. Skóra M, Bulanda M, Jagielski T. (2015). In vitro activities of a wide panel of antifungal drugs against various Scopulariopsis and Microascus species. Antimicrob Agents Chemother. 59:5827-5829. doi: 10.1128 /AAC.00978-15.
24. Sri-Jayantha L, Matthews KG, Scharf V. (2019). Scopulariopsis brevicaulis rhinosinal infection in a dog. J Am Anim Hosp Assoc. 55: e55102 doi:10.5326/jaaha-ms-6869 Tosti A, Piraccini BM, Stinchi C, Lorenzi S. (1996). Onychomycosis due to Scopulariopsis brevicaulis: clinical features and response to systemic antifungals. Br J Dermatol. 135(5): 799-802. doi: 10.1111/j.1365-2133.1996.tb03895.x
25. Yang Q, Wei J, Chen Z. (2012). Fatal bronchial invasion of Scopulariopsis brevicaulis in an acute monocytic leukemia patient. Diag Microbiol Infect Dis.73(4):369-371. doi: 10.1016/j.diagmicrobio. 2012.04.010