Oleanolik asidin çekal ligasyon ve punksiyon ile sepsis oluşturulan ratlarda kalp hasarı üzerine etkileri

Öz

Bu çalışmada zeytin ağacı (Olea europeae) ağacının yaprağından saflaştırılan oleanolik asidin (OEA) ratlarda çekal ligasyon ve punksiyonla (CLP) indüklenen sepisisin oluşturduğu kalp hasarı üzerine etkileri incelendi. Her gruba eşit olacak şekilde 30 adet Wistar erkek rat sham, sepsis, OEA-150, OEA-300 ve CEFT olarak 5 gruba ayrıldı. Kromatografik metotlarla zeytin yaprağından saflaştırılan oleanolik asit OEA gruplarına 150 ve 300 mg/kg dozlarda oral yolla uygulandı. CEFT grubuna seftriakson 150 mg/kg dozda intraperitoneal yolla uygulandı. Uygulamadan 24 saat sonra ratlardan kan serumu ve kalp dokuları, histopatolojik, immünohistokimyasal ve biyokimyasal analizler için alındı. Yapılan histopatolojik ve immünohistokimyasal incelemede, sepsis grubunun doku tümör nekrozis faktör (TNF)- ve interlökin (IL)-1β ekspresyonlarında artış, myokartta kas liflerinde şiddetli düzeyde hyalin dejenerasyon, Zenker nekrozu ve damarlarda şiddetli düzeyde hiperemi gözlendi. OEA ve seftriakson (CEFT) sitokin ekspresyonlarında ve histopatolojik bulgularda azalma sağladı. Sepsis, doku superoksit dismutaz aktivitesini (SOD) azalttı. Ayrıca lipid peroksidasyonu (LPO) ve glutatyon (GSH) seviyeleri ile doku katalaz (KAT), serum kreatin kinaz (CK-MB) ve serum laktat dehidrojenaz (LDH) aktivitelerini önemli şekilde arttırdı. OEA ve CEFT, SOD aktivitesini önemli derecede arttırırken diğer parametrelerde belirgin düşüşe neden oldu. Bu çalışmadan elde edilen bulgular ışığında oleanolik asidin sepsis sırasında oluşan kalp dokusu hasarının azaltılmasında faydalı olabileceği düşünülmektedir.

Anahtar Kelimeler

• İnflamasyon
• Kalp
• Oleanolik asit
• Seftriakson
• Sepsis

The effects of oleanolic acid on heart injury in cecal ligation and puncture-induced sepsis in rats

Öz

In the present, the effects of oleanolic acid (OEA) purified from leaf of the olive tree (Olea europeae) on heart damage caused by cecal ligation and puncture (CLP) induced sepsis model in rats were examined. Thirty Wistar rats equally were divided into six groups as sham, sepsis, OEA-150, OEA-300 and CEFT. Oleanolic acid purified from olive leaf by chromatographic methods was orally administered at doses of 150 and 300 mg/kg to OEA groups. Ceftriaxone was intraperitoneally administered at a dose of 150 mg/kg to the CEFT group. Twenty-four hours after the application, blood serum and heart tissues were taken from rats for histopathological, immunohistochemical and biochemical analysis. In histopathological and immunohistochemical examination, an increase in tissue tumor necrosis factor (TNF)- and interleukin (IL)-1β expressions of the sepsis group, severe hyaline degeneration in muscle fibers in the myocardium, Zenker necrosis and severe hyperemia in the vessels were observed. OEA and ceftriaxone (CEFT) provided a decrease in cytokine expressions and histopathological findings. Sepsis reduced tissue superoxide dismutase activity (SOD). It also significantly increased lipid peroxidation (LPO), glutathione (GSH) levels, catalase (CAT), serum creatine kinase (CK-MB) and serum lactate dehydrogenase (LDH) activities. OEA and CEFT significantly increased SOD activity while causing a significant decrease in other parameters. In the light of the findings from this study, it is thought that oleanolic acid may be beneficial in reducing heart tissue damage during sepsis.

Anahtar Kelimeler

• Ceftriaxone
• Heart
• Inflammation
• Oleanolic acid
• Sepsis

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