Year 2019, Volume 30 , Issue 1, Pages 70 - 77 2019-06-30

Enfeksiyöz Nekrotik Hepatitis Aşısının Üretiminde Poli (D, L-Laktik-Ko-Glikolik Asit) (PLGA) Biyopolimerinin Adjuvant Etkisinin Araştırılması

Zehra AKINCI [1] , Hakan KALENDER [2]


Bu çalışmada, koyunların enfeksiyöz nekrotik hepatitis hastalığına karşı alüminyum hidroksit ve poli (D, L-Laktik-Ko-Glikolik Asit) (PLGA) adjuvantları kullanılarak üretilen aşıların kobaylarda oluşturduğu bağışıklık düzeyleri karşılaştırıldı. Clostridium novyi tip A kültürünün formol ile inaktivasyonundan sonra, alüminyum hidrokside adsorbe edilmiş ve çift emülsiyon çözücü buharlaştırma yöntemi uygulanarak PLGA (laktid/glikolid oranı: (50/50), moleküler ağırlık: 30.000-60.000 dalton) ile enkapsüle edilmiş antjen içeren iki farklı toksoid aşı hazırlandı. Aşıların oluşturduğu bağışıklık düzeyini belirlemek için 5-6 aylık ve ağırlıkları 400-500 gram olan erkek kobaylar kullanıldı. Her biri 10 kobaydan oluşan 3 grup oluşturuldu. Birinci gruptakilere 21 gün arayla çift doz (2 ml+2ml) alüminyum hidroksitli aşı, ikinci gruptakilere PLGA mikrosferli aşı tek doz (2 ml) ve üçüncü gruptakilere PLGA mikrosferli aşı yarım doz (1ml) derialtı yolla verildi. Birinci gruptaki kobaylardan rapel aşılamadan, ikinci ve üçüncü gruptaki kobaylardan tek doz aşılamadan sonraki 15, 30 ve 45. günlerde kalpten kan örnekleri alınarak havuzlanmış serum örnekleri elde edildi. Kan serumlarındaki antikor düzeyi fare Toksin Nötralizasyon Test (TNT) ile belirlendi. Tek doz mikrosferli aşı ile çift doz alüminyum hidroksitli aşı uygulamalarından sonraki  30. ve 45. günlerde aynı düzeyde antikor (8 IU/ml) saptandı. Ancak 15. günde çift doz alüminyum hidroksitli aşının antikor düzeyi 4 IU/ml iken, tek doz PLGA mikrosferli aşının antitor düzeyi 2 IU/ml olarak bulundu. Yarım doz PLGA mikrosferli aşı verilen kobaylarda yeterli düzeyde (antikor titresi˂2.5 IU/ml) bağışıklık elde edilemedi. Sonuç olarak,  tek dozlu veteriner aşıların geliştirilmesi amacıyla  farklı polimer tipi ve enkapsulasyon yöntemleri kullanılarak daha geniş kapsamlı çalışmaların yapılması gerekmektedir. 
C. novyi, aşı, adjuvant, PLGA
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Primary Language tr
Subjects Science
Journal Section Original Article
Authors

Orcid: 0000-0002-0713-0989
Author: Zehra AKINCI
Institution: Sanovel İlaç San. ve Tic. A.Ş., İstanbul, Türkiye
Country: Turkey


Orcid: 0000-0003-4653-1942
Author: Hakan KALENDER (Primary Author)
Institution: Fırat Üniversitesi Mühendislik Fakültesi, Biyomühendislik Bölümü, Elazığ, Türkiye
Country: Turkey


Dates

Publication Date : June 30, 2019

APA AKINCI, Z , KALENDER, H . (2019). Enfeksiyöz Nekrotik Hepatitis Aşısının Üretiminde Poli (D, L-Laktik-Ko-Glikolik Asit) (PLGA) Biyopolimerinin Adjuvant Etkisinin Araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi , 30 (1) , 70-77 . DOI: 10.35864/evmd.543182