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Blue tongue and blue tongue vaccine- modified Iive virus - chich embryo origin- vacumm dried

Year 1962, Volume: 1 Issue: 5, 402 - 411, 01.06.1962

Abstract

Blue Tongue vaccine was prepared at Foot and Mouth Disease
Serology and Tissue Culture Laboratory of Etlik Baderiology
Institute· Ankara, for experimental study.

To prepare the vaccine 14 of several strains of the virus were
brought from Mexico. Mossap strain which had been suitably attenuated by 60 serial passages in embryonating chicken eggs was
used in this study.
Fertile eggs, preincubated for 8 days, were inoculated with
0,2 ml of 1/ 10 dilution of Mossap strain via yolk sac route. Death of
embryos duringthe first 48 hours after inoculation, were discarded.
Observation continued for 5 days. The contents of the eggs of
embryos which haddiedwithin 3th. to 5th. days postinoculation,
were collected. The contents of the eggs were mixed with equal
volume of Buffer- Lactose- Pepton medium, 100 units of penicilline
and 100 microgram streptomycin per milliter were also added.


The mixture was dispensed in 0,75 ml quantities into each ampule
and desiccated in Freeze- Dryer- appartus. The titration of
the vaccine examined by the method of Reed and Muench before
and after desication. It was calcullated that the titration before
desiccation was 10-6,2 after desiccation was 10-5,6

References

  • 1 - CAMERON G. 1950) Tissue Culture Technic, 2nd. ed. Newyork.
  • 2 - ERDÖL Z. A. (1945) Koyunlarda mavi dil (Bluetongue) hastalığı, Türk Veteriner Cemiyeti yayını.
  • 3- FRANZ HUTRA, JOSEPH MAREK and RUDOLPH MANNIGER (1949> GER (1949) Blue Tongue.
  • 4 - GORHAM J. R. (1957) Asimple technic for the inoculation of chorio- allantoic- membrane of chicken embryos. Am. J . Vet. Res. 18 : 891-692.
  • 5 - GRAY D. P. and BANNiSTER G. L. (1961) Melaphagus ovinus, Veterinary Science, Sept.
  • 6 - HAIG (1956) The cyto- pathogenic action of bluetongue virus on tissue cultures. Onderstepoort J . Vet. Res. 27/171-177.
  • 7- IV AL A. MERCHANT- PACKER, 1956 Veterinary Bacteriology and Virology,
  • 8 - KALMER, SPAULDING, ROBINSON (1951) Approved Laboratory Technic, Fifth edition
  • 9- KERCHER, D. C. Mc. KRORK B. R. Mc. and J. L. HOURRIGAN (1956) Blue Tongue of sheep, Animal Diseases.
  • 10 - LORANT KEMENY, LOYD E. DREHLE (1961) The use of Tissue Culture- propagated bl u e tongue virus for vaccine Veterinary Research, Volume XII, Sept.
  • 11 - MOUL TON, W. (1962) F. A. O. Ankara Mümessili, Şahsi komünikasyon.
  • 12 - REED L. J. and MUENCH H. (1938) A simple method of estimating fifty percent endpoints.. Am. J., Hyg. 27/493-497
  • 13- TAYLOR, R. M. (1952) Studies on certain viruses isolated in the tropics of Africa and South America : Their grawth and behavior in the embryonaed hen egg. J. Immunal 68/473-497.

Koyunlarda Mavi Dil Hastalığı ve Aşı Hazırlama Tekniği

Year 1962, Volume: 1 Issue: 5, 402 - 411, 01.06.1962

Abstract

Tecrübevi çalışma maksadı ile Ankara- Etlik Bakteriyoloji
Enstitüsü Şap Seroloji ve Doku Kültürü Laboratuvarında mavi dil
aşısı hazırlanmıştır.
Aşının hazırlanması için 14 çeşitli suç Meksikadan getirtilmiştir.
Bu çalışmada 60 pasajla yumurta embriyosuna adapte edilmiş
Mossap suşu kullanılmıştır.
8 günlük embriyonl uyumurta sarısına (BTV) Mossap suşunun
1/10 dilusyonundan 0,2 cc. inakule edilmiştir. İnokulasyondan sonra
48 saat zarfında ölenler nazarı itibare alınmamıştır. Müşahade inakulasyondan sonra 5 gün devam etmiştir. İnokulasyondan sonra
3 - 5 gün içinde ölen yumurtaların muhtevisi toplanmıştır. Bu yumurta
muhtevası aynı miktar Buffer- Laktoz- Pepton vasatı ile karıştırılmıştır. Herbir cc. için 100 ünite penicilline ve 100 mikrogram
streptomycin ilave edilmiştir.
Bu karışım 0,75 cc. miktarında ampullere taksim edilmiş ve freeze-
dryer'de kurutulmuştur. Aşı titrasyonu Reed ve Muench metoduna
göre kurutulmadan evvel ve sonra olmak üzere iki defa yapılmıştır.
Kurutmadan evvelki titrasyona 10-6,2 kurutmadan sonraki
titrasyon 10-5,6 olarak tesbit edilmiştir.
Aşının muafiyet denemeleri henüz yapılmamıştır.

References

  • 1 - CAMERON G. 1950) Tissue Culture Technic, 2nd. ed. Newyork.
  • 2 - ERDÖL Z. A. (1945) Koyunlarda mavi dil (Bluetongue) hastalığı, Türk Veteriner Cemiyeti yayını.
  • 3- FRANZ HUTRA, JOSEPH MAREK and RUDOLPH MANNIGER (1949> GER (1949) Blue Tongue.
  • 4 - GORHAM J. R. (1957) Asimple technic for the inoculation of chorio- allantoic- membrane of chicken embryos. Am. J . Vet. Res. 18 : 891-692.
  • 5 - GRAY D. P. and BANNiSTER G. L. (1961) Melaphagus ovinus, Veterinary Science, Sept.
  • 6 - HAIG (1956) The cyto- pathogenic action of bluetongue virus on tissue cultures. Onderstepoort J . Vet. Res. 27/171-177.
  • 7- IV AL A. MERCHANT- PACKER, 1956 Veterinary Bacteriology and Virology,
  • 8 - KALMER, SPAULDING, ROBINSON (1951) Approved Laboratory Technic, Fifth edition
  • 9- KERCHER, D. C. Mc. KRORK B. R. Mc. and J. L. HOURRIGAN (1956) Blue Tongue of sheep, Animal Diseases.
  • 10 - LORANT KEMENY, LOYD E. DREHLE (1961) The use of Tissue Culture- propagated bl u e tongue virus for vaccine Veterinary Research, Volume XII, Sept.
  • 11 - MOUL TON, W. (1962) F. A. O. Ankara Mümessili, Şahsi komünikasyon.
  • 12 - REED L. J. and MUENCH H. (1938) A simple method of estimating fifty percent endpoints.. Am. J., Hyg. 27/493-497
  • 13- TAYLOR, R. M. (1952) Studies on certain viruses isolated in the tropics of Africa and South America : Their grawth and behavior in the embryonaed hen egg. J. Immunal 68/473-497.
There are 13 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Sciences
Journal Section Original Article
Authors

Erdoğan Finci This is me

Güngör Okay This is me

Publication Date June 1, 1962
Submission Date June 1, 1962
Published in Issue Year 1962 Volume: 1 Issue: 5

Cite

APA Finci, E., & Okay, G. (1962). Koyunlarda Mavi Dil Hastalığı ve Aşı Hazırlama Tekniği. Etlik Veteriner Mikrobiyoloji Dergisi, 1(5), 402-411.

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