Abstract
Separation and identification of amphetamine and its homologues
and other organic bases used in veterinary medicine as sympathomimetics
were performed by paper chromatography. The substances were
eluted by (6: 1,5 : 2,5) bunatol/acetic acid/water mixture, The experiment
were carried on by_ isolating the compounds from horse urine and
pure substance mixtures by paper chromatography. The spots _ were
classified as follows, (a) fluorescent (b) abs orbing (c) non reakting
under UV light source The extractıon of the substances from urine was
performed by ammonium sulphate method. The Rf values obtained for
ur'ine were found lower than those of the pure substances. The spots
from the two soureces were not identical when examined under UV. The
results obteined from the pure and urinary substances corresponted to
other when the urinary was purified 2-3 times, which is explained by
the existence of other compounds interfering with the chromatoğraphic
separation.
References
- 1- - Clake, E.G.G. (ı959) : New appaatus a two stage ınloro evaperature. Jour. · Pharın. and Phaın anology. ı2 ; 509- 511.
- 2-Clarke, EGC. (1962) : The doping of racehorses. Jour. Med. Leg. 30; ı8ı-ı85.
- 3- Clarl{e, E.G.C. - Sowter, S.A. (ı964) : Coııtinuous flow spottig for paper chroınatography . Nature.• 202 ; 795- 796. ::
- 4 - Curry, A.S.- Powwel, H. (1954) : Paperchroınatographyc exarnination of the , alkolold extract in toxicology. Nature. ı73; 1143. ' ' · :: :
- 5 Cury, A.S. (1960) : Toxicologycal analysis. Jour Pharma. and Phannacology ı2 ; 32ı ~ 339. ~. '·'
- 6 - Goodman, L.S. (1958) The Pharrnacological basis of therapeutions. The Macınillan Company. Newyork pp. 5ıO- 533.
- 7 - Kantemir, İ. (1960) : Teda vinin e sası farrnalwloji. Ankara Üniversitesi Tıp Fakültesi yayınları p. 207.
- 8 - Lumlquist, F. (1962) : Methoçls of forensic science. ı. Intersciencepublishers NewyorkjLondon. Pp. 3ı, 32- 41- 33.
- 9 - Macka.y, A. (1961) : S o ın e effects of drugs used in doplng. New. Ze Vet. Jour. 9, ı29- ı31.
- 10 - Merck lndex (1960) : Chernicals and drugs. Mer ck Co. Ine Rahway, · H.J. U.S.A. Pp. 23, 59, 72, 328, 403, 430.
- 11 - Milks, H.J. (1949) : Pra ctical Vet erinary pharınacology, m,a teria rnedica and therapeuticks. Bailliers, Tindall and Co. London. p:'' '113.' ·
- 12 - Moore, P.A. (1957) : Detection of doping Vet. Rec. 69: ı079 -ı083.
- 13 - Nickolls, L.C. (1946) : The scientific investigation of erime. Butterwortıi Co. London Pp . . 374 - 382: ·' · . ·
- 14 - Peterson, N. (1964) .: Methods for calleetion and analysis of horse sallvo and urine for dedection of drugs. 148 07 Hillside Avenue Newyork, U.S.A.
- 15 - Smith (1960) : Ca rorna tographio and electrophoretic techniques. William Heines Medical books. London. Vol. ı . F'p . 84, 298.
- 16 Smith, L.F. (1961) : The ı~hanging pattern of doping in horse racing and its control, New. Ze. Vet. Jour. 9; 121, 128. 1
- 17 - Stewart, _ Ç.P.) 1961) : Toxicology. Vol. ı. Academic press. London Pp. 547, 570 .
Amphetamin ve benzeri maddeleri kağıt kromatografisi yardımiyle birbirinden ayırmağa yarayacak ayraçların bulunmasıı
Abstract
1 - Denemeye alınan üç adet inkişaf solusyonundan butanol/ glasıel
asetik asit/su (6 : ı,s: 2,5) karı şımı ile en iyi sonuç alınmıştır .
2 - Saf ve at idrarına ilave edilmiş olunan on adet çeşitli amphetamin
ve benzeri maddeler için Rf değeri ve ultraviyole ışını altındaki
görüntüleri tesbit edilmiştir.
3 - Kromatoğramlar inkişaf solusyonunda ı2 saat müddetle bekletilmeleriyle
alkalotilerin daha iyi ayrılabileceği anlaşılmıştır.
4 - Amphetamin ve benzeri maddeleri biribirinden ayırmak ıçın
16 adet değişik ayraç kullanılmıştır. Bunlarla denemede kullandığımiz
ı O adet değişik amphetamin ve amhetamin benzeri maddeleri kesinlikle
biribirinden ayırmak mümkün olmuştur.
Ayni kromotoğramlara, maddelerin saf şekilleri kontrol gayesile
tatbik edilerek çalışmalar mukayeseli olarak yürütülmüştür. idrardan
izole edilen alkaloiderin Rf değerleri saf şekillerine göre daha küçük
bulunmuştur. Fakat ikinci bir temizleme işleminden sonra, idrardan elde
edilen alkaloiderin Rf değerleri saf şekillerinin Rf değerlerine ulaşmıştır
.
Keywords
References
- 1- - Clake, E.G.G. (ı959) : New appaatus a two stage ınloro evaperature. Jour. · Pharın. and Phaın anology. ı2 ; 509- 511.
- 2-Clarke, EGC. (1962) : The doping of racehorses. Jour. Med. Leg. 30; ı8ı-ı85.
- 3- Clarl{e, E.G.C. - Sowter, S.A. (ı964) : Coııtinuous flow spottig for paper chroınatography . Nature.• 202 ; 795- 796. ::
- 4 - Curry, A.S.- Powwel, H. (1954) : Paperchroınatographyc exarnination of the , alkolold extract in toxicology. Nature. ı73; 1143. ' ' · :: :
- 5 Cury, A.S. (1960) : Toxicologycal analysis. Jour Pharma. and Phannacology ı2 ; 32ı ~ 339. ~. '·'
- 6 - Goodman, L.S. (1958) The Pharrnacological basis of therapeutions. The Macınillan Company. Newyork pp. 5ıO- 533.
- 7 - Kantemir, İ. (1960) : Teda vinin e sası farrnalwloji. Ankara Üniversitesi Tıp Fakültesi yayınları p. 207.
- 8 - Lumlquist, F. (1962) : Methoçls of forensic science. ı. Intersciencepublishers NewyorkjLondon. Pp. 3ı, 32- 41- 33.
- 9 - Macka.y, A. (1961) : S o ın e effects of drugs used in doplng. New. Ze Vet. Jour. 9, ı29- ı31.
- 10 - Merck lndex (1960) : Chernicals and drugs. Mer ck Co. Ine Rahway, · H.J. U.S.A. Pp. 23, 59, 72, 328, 403, 430.
- 11 - Milks, H.J. (1949) : Pra ctical Vet erinary pharınacology, m,a teria rnedica and therapeuticks. Bailliers, Tindall and Co. London. p:'' '113.' ·
- 12 - Moore, P.A. (1957) : Detection of doping Vet. Rec. 69: ı079 -ı083.
- 13 - Nickolls, L.C. (1946) : The scientific investigation of erime. Butterwortıi Co. London Pp . . 374 - 382: ·' · . ·
- 14 - Peterson, N. (1964) .: Methods for calleetion and analysis of horse sallvo and urine for dedection of drugs. 148 07 Hillside Avenue Newyork, U.S.A.
- 15 - Smith (1960) : Ca rorna tographio and electrophoretic techniques. William Heines Medical books. London. Vol. ı . F'p . 84, 298.
- 16 Smith, L.F. (1961) : The ı~hanging pattern of doping in horse racing and its control, New. Ze. Vet. Jour. 9; 121, 128. 1
- 17 - Stewart, _ Ç.P.) 1961) : Toxicology. Vol. ı. Academic press. London Pp. 547, 570 .
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Sciences |
| Journal Section | Original Article |
| Authors | |
| Publication Date | June 1, 1967 |
| Submission Date | December 1, 1967 |
| Published in Issue | Year 1967 Volume: 3 Issue: 3-4 |