Koçlarda Aglutinasyon ve Agar- Jel Diffuzyon Yöntemiyle Brucella Ovis Antikorlarının Saptanması Üzerinde Araştırmalar

Yıl 1984, Cilt: 5 Sayı: 6-7, 41 - 62, 01.12.1984

Mübeccel Yumuşak

Öz

Bu araştırma.da kullanılan 205 koç serumundan 185'i Et ve Balık
Kurumu'na, Konya Cihanbeyli kazasından kesime gelen hayvanlardan,
15'i Enstitümüzde deneme hayvanı olarak kullanılan koçlardan
ve 5'i de Adana Mercimek Harasından Lôboratuvarımıza muayene
için gelen koç serumlarından sağlanmıştır. Bu serumlar aynı zamanda
serum aglutinasyon ve agar jel diffuzyon test yöntemiyle Bruceila
ovis'e karşı yoklanmış olup serum aglutinasyon testinde 166
serum 1/10-1/640 ara.sında değişen aglutinin titreleri göstermiştir.
Bu testte 43 serum pozitif, 6 şüpheli ve 156 serum negatif reaksiyon
verirken, Agar jel diffuzyon testinde 50 serum pozitif, 155 serum da
negatif sonuç vermiştir. Aglutinasyon testinde şüpheli 5 serum negatif,
3 serum agar jel diffuzyon testinde pozitif reaksiyon vermiştir.
Yine agar jel'de negatif olan bir serum aglutinasyonla pozitif sonuç
vermiştir. 42 serum iki testte de parelel çalışmış olup uyum yüzde
% 84 dür. Buna. göre agar jel diffuzyon testinin daha kesin sonuç
vermesi, bu testin duyarlı, basit ve ekonomik oluşuyla serolajik kanıda
önemli bir yeri olduğu kanısına varılmıştır.

Anahtar Kelimeler

Koç, Brucella Ovis, Brucella Ovis Antikor Saptanması, Mübeccel YUMUŞAK, 1984

Studies on aglutination and agar- gel diffusion test for the detection of antibodies against Brucella ovis in rams.

Öz

This study ıwas carried out using 205 blood serum of ram
collected mainly from Konya, Ankara and Adana proices of the
country. All serums ware examined both by serum aglutination and
agar-gel diffusion tests comparisonly Br. ovis By serum aglutination
tests 166 serums showed 1/10-1/640 agglutinin titres among them
43 serum !were positives, 156 negatif and 6 suspicion reactions
were obtained. By agar gel diffusion test, 50 serum gave positive
reactions and 155 ıwere negatives from 5 serums of suspicion and
from 3 negatives reactions by agglutination tests were positives by
agar gel diffussion tests on the otherland one serum sample showed
negative reactions by agar gel diffuion test ıwas positive by
agglutination test 42 (84) of the pasitives serums ıwere corresponded
by both tıwo tests used.
it seemed to be a.gar gel diffusion tests more sensitive simplier
and economic, a.nd more valid than aglutinin tests for Bruceila ovis.

Kaynakça

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