Detection of antibodies against Egg drop syndrome-1976 (EDS'76) in yolk and serum of vaccinated, nonvaccinated chickens by haemagglutination-inhibition (HI) test

Year 1988, Volume: 6 Issue: 2, 57 - 64, 01.12.1988

Ömer Akay Müjgan İzgür Hatice Ayhan

Abstract

Studler laying hens that lacked haemagglutination-inhibition
antibody were vaccinated intramuscullary with :the EDS'76 vaccine.
Before and after vaccination, 300 sera and 300 yolks were collected
from chickens. Antibodies were not detected in sera of 150 and
in yolks of 150 of unvaccinated hens by HI tests. In the vaccinated
group, 150 sera were positive in the EDS'76 HI test, with titres
ranging from log2 5-13 and 150 yolks treated with chloroform were
positive in .the same test, with titres ranging from log2 3-11. A good
corelation was observed between serum and yolk titers. Results
of this study show that yolk from EDS'76 vaccinated hens is readily
used instead of serum for detection of antibodies against EDS'76.

I.Egg Drop Syndrome-1976 (EDS'76)'ya Karşı Aşılı ve Aşısız Tavukların Yumurta Sarısı ve Serumlarındaki Antikorların Hemaglutinasyon-İnhibisyon (HI) Testi ile Belirlenmesi

Abstract

EDS'76 virusuna karşı HI antikorları taşımayan Studler ırkı
yumurta tavukları EDS'76 aşısı ile aşılanmışlardır. Aşılamadan önce ve aşılamadan sonra hayvanlardan 150'şer olmak üzere toplam
300 serum ve 300 yumurta alınarak EDS'76 yönünden HI testi uygulanmış,
aşılamnamış .grupta serum ve yumurta sarısı HI titreleri
negatif bulunmuştur. Aşılanmış grupta . ise; serum HI titreleri
log2 5-13, yumurta sarısı HI titrelerinin log2 3-11 arasında değiştiği
ve aralarında uygun bir korelesyon olduğu saptanmıştır. Alınan sonuçlara
göre EDS'76'ya karşı oluşan antikorları saptama da serum
yerine yumurta sarısının kullanılalbileceği belirlenmiştir.

Keywords

I. Egg Drop Syndrome, Yumurta Azalması Sendromu, 1976 (EDS'76), EDS'1976, EDS'76, Yumurta, Tavuk, Yumurta Sarısı ve Serumlarındaki Antikor, Hemaglutinasyon - İnhibisyon Testi, HI Testi, Ömer AKAY, Müjgan İZGÜR, Hatice AYHAN, 1988

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