İn this study, tuberculosis found in lebistes fishes was investigated. In histological examination the tubercles were observed in the definite areas of the liver, spleen, and peritoneum of the diseased and dead fishes.
Isolated microorganism, Mycobacterium marinum showed a positive hydrolysis in Tween hydrolysis test but showed negative reaction in catalase and nitrate tests. Isolated microorganisms did not grow on Nitrite-Sauton agar and Picric acid-Sauton agar but growth on PAS NH2OH (2 mg/ml), PNB, and Salicylate medium.
Kaynakça
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AMLACHER, E. (1968) : Experimentella Befunde zur pathologie und ·Bakteriologie
der Fischtuberkulose. Z. ·Fisoherei. '16: 1-30.
AMLACHER, E. (1981) : Taschen'buch der Fischkrankteiten. 4 Aufl. Gustav
Fischer Verlag, Stuttgart.
ANONIM. (1985) : Laboratory methods in Veterinary Microıhlology ·for the
isolation and identification of Myco'bacteria. Ames.
BESSE, P. {1949) : Epizootia a bacilles acido-resistants chez des poissons
exotiques. Bull. Acad. Vet. France. 22; 151-154.
BESSE, P (1949) Ouellques affections a bacilles acido-resistants ohez les
poecilothermes vivant en equarium. Bull. Acad. Vet. France. 22: 425-431 .
CSABA, G.J., KOVACS, L., BEKE·SI, S., TUBOLY, S., BANK·I, M. und KORMENDI,
B. (1982) : Mycobacteriose der Fische. Massenerkrankung in einer
Macropodus operculais. Zucht. Riv lt. Piscic. lttiop, 17(2) : 84-87.
GANOADHARAM, P.R. and DRAUBI, A.J. (1973) : Susceptibility of myco.
bacteria to p-nitrc1benzoic acid in relation to their niacin production. Amer.
Rev. Resp. Dis., 108: 143-146.
KUBICA, G.P., 'ONıES, W . .O., ABDOTT, V.D., BEOM, R.E., KILBURN, J.O. and
OATER J.C. (1966) : Differential identification of mycobacteria. Tests on
catalase activity. Amer. Rev. Res. Dis., 94: 400-405.
NIGRELLI, R.F. and VOGEL, H. ( ı1963) : Spontaneous tuberculosis in fisches
and in other cold"blooded verteıbwtes with special reterence to Mycobacterium
fortuitum CRUZ from fish and human lesions. Zo·ologica. 48: 131-144.
PARISOT, P.J. (·1958) : Tuberculosis in fish. A relew of the literature with
discription of the disea·se in salmonid fish. Bacteriol. Rev., 22: 240-245.
REICHANBACH-KLINKE. H.H. (1980) : Kranheiten und Sohadıggungen ds
Fisohe. 2. Aulf. Gustav Fischer, Verlag, Stuttgard.
TSUKAMURA. M. (1962) Carotenoid of photochroınogen of «unclassified
ınycobacteria » . J . Biacheın . , 51 : 169-171.
TSUKAMURA, M. (1965) : Differentiation of ınycobacteria <by picric acid
toler.ance. Amer. Rev. Resp. Dis., 92: 491-492.
TSUKAMURA, M. (1965) : Differentiation of mycobacteria. by susceptibility
to hl{droxyamine and 8-azo-quanine. J. Bact., 9: 556-·557.
TSUKAMURA, M. (1965) : Salicylate degregation test for differentiatian of
Mycdbacterlum fortuitum from other mycobacteria. J. Gen. Microbiol., 41:
309-315.
TSUKAMURA, M. (1967) : ldentification of mycobacteria. Tubercle (London).
48: 311-338.
TSUKAMURA, .M . ('1975) : ldentification of mycobacteria. Researoh Labomtory
of the NatiOnal Sanatoriuın . Chubu ohest Hospital. Obu, Aiohi-ken,
Japon.
TSUKAMURA, M. and TSUK!J\MURA, A.S. (1968) : Dif.ferentiation of mycobacteria
by suscepti'bility to nitrite and propylenglycol. A:mer. Rev. Res. Dis ..
98: 505-506.
VOGEL , H. (1958) : Mycobacteria from cold-bloodded animals. Amer. Rev.
Juıbl. Pul. Dis., 77: 283-838.
WAYNE, LG., DOUBEK, C.V. and RUSSELL, R.L. (1964) : Classification and
identification of mycobacteria. 1. Tests employing Tween 80 as substrate.
Amer. Rev. Res. Dis., 90: 588-597.
Bu çalışmada Lebistes balıklarında görülen tüberküloz olgusu incelenmiştir. Hasta balıkların yapılan histopatolojik yoklamasında, karaciğer, dalak ve periton'un belirli bölgelerinde epiteloid histiositlerin oluşturduğu tuberküllere rastlanmıştır.
Organlardan izole edilen Mycobacterium marinum'um PAS NH2OH (25 mg/ml), PNB, Salisilat ortamlarında ürediği. Tween hidroliz testinde pozitif, Katalaz, Nitrat testlerinde negatif, Nitrit-Sauton agar ve Pikrik asi-Sauton agarda üremedği saptanmıştır.
CSABA, G.J., KOVACS, L., BEKE·SI, S., TUBOLY, S., BANK·I, M. und KORMENDI,
B. (1982) : Mycobacteriose der Fische. Massenerkrankung in einer
Macropodus operculais. Zucht. Riv lt. Piscic. lttiop, 17(2) : 84-87.
GANOADHARAM, P.R. and DRAUBI, A.J. (1973) : Susceptibility of myco.
bacteria to p-nitrc1benzoic acid in relation to their niacin production. Amer.
Rev. Resp. Dis., 108: 143-146.
KUBICA, G.P., 'ONıES, W . .O., ABDOTT, V.D., BEOM, R.E., KILBURN, J.O. and
OATER J.C. (1966) : Differential identification of mycobacteria. Tests on
catalase activity. Amer. Rev. Res. Dis., 94: 400-405.
NIGRELLI, R.F. and VOGEL, H. ( ı1963) : Spontaneous tuberculosis in fisches
and in other cold"blooded verteıbwtes with special reterence to Mycobacterium
fortuitum CRUZ from fish and human lesions. Zo·ologica. 48: 131-144.
PARISOT, P.J. (·1958) : Tuberculosis in fish. A relew of the literature with
discription of the disea·se in salmonid fish. Bacteriol. Rev., 22: 240-245.
REICHANBACH-KLINKE. H.H. (1980) : Kranheiten und Sohadıggungen ds
Fisohe. 2. Aulf. Gustav Fischer, Verlag, Stuttgard.
TSUKAMURA. M. (1962) Carotenoid of photochroınogen of «unclassified
ınycobacteria » . J . Biacheın . , 51 : 169-171.
TSUKAMURA, M. (1965) : Differentiation of ınycobacteria <by picric acid
toler.ance. Amer. Rev. Resp. Dis., 92: 491-492.
TSUKAMURA, M. (1965) : Differentiation of mycobacteria. by susceptibility
to hl{droxyamine and 8-azo-quanine. J. Bact., 9: 556-·557.
TSUKAMURA, M. (1965) : Salicylate degregation test for differentiatian of
Mycdbacterlum fortuitum from other mycobacteria. J. Gen. Microbiol., 41:
309-315.
TSUKAMURA, M. (1967) : ldentification of mycobacteria. Tubercle (London).
48: 311-338.
TSUKAMURA, .M . ('1975) : ldentification of mycobacteria. Researoh Labomtory
of the NatiOnal Sanatoriuın . Chubu ohest Hospital. Obu, Aiohi-ken,
Japon.
TSUKAMURA, M. and TSUK!J\MURA, A.S. (1968) : Dif.ferentiation of mycobacteria
by suscepti'bility to nitrite and propylenglycol. A:mer. Rev. Res. Dis ..
98: 505-506.
VOGEL , H. (1958) : Mycobacteria from cold-bloodded animals. Amer. Rev.
Juıbl. Pul. Dis., 77: 283-838.
WAYNE, LG., DOUBEK, C.V. and RUSSELL, R.L. (1964) : Classification and
identification of mycobacteria. 1. Tests employing Tween 80 as substrate.
Amer. Rev. Res. Dis., 90: 588-597.