The properties of protein-A. productlon by Staphylococci isolated from poultry and the importance of two different media on the production of protein-A.

Yıl 1990, Cilt: 6 Sayı: 6, 61 - 66, 01.12.1990

Hatice Ayhan Nejat Aydın

Öz

In this study, 126 S.aureus and 118 S.epidermidis isolates re
·covered from poultry were tested by passive haemagglutination test
using· sensitized sheep red blood cells on slide. In the passages of
isolates from TSA to TSA media 16 (12.7 %) of S.aurues, 2 (1.7 %)
of . S.epidermidis were determined as . positive. · ın subcultures made from TSA to Gox media 45 (35.7%) and 6. (5%) strains representing S.aureus and S.epidermidis were found positive for the protein-A, respectively; These results showed that Gox agar, was the · most sultable medium for the production of protein-A by staphylococci.

Tavuk Orjinli Stafilokok Suşlarının Protein-A Sentezleme Özellikleri ve Protein-A Sentezi Üzerinde İki Farklı Besiyerinin Önemi

Öz

İdentifiye edilen tavuk orijinli 126 S.aureus ve 118 S.epidermidis
suşu TSA ve Gox agar besiyerlerinde protein-A sentezlemeleri yönünden test edildi. Duyarlı eritrositlerin kullanıldığı lam aglutinasyon testinde, TSA besiyerinden TSA besiyerine yapılan subkültürlerde S.aureus'ların 16 (% 12.7'sı, S.epidermidis suşlarının 2 (% 1.7)'si; TSA besiyerinden Gox besiyerine yapılan subkültürlerde ise· S.aureus ve S.epidermidis suşlarının sırasıyla, 45 (% 35.7) 'i, 6 (% 5)'i pozitif bulundu.
Protein-A sentezleme üzerine en uygun besiyerinin Gox agar
olduğu belirlendi.

Anahtar Kelimeler

Tavuk Orjinli Stafilokok, Stafilokok, Stafilokok Suşları, Protein-A Sentezleme, Stafilokok Suşlarının Protein-A Sentezlemesi, Protein-A Sentezinde İki Farklı Besiyerinin Önemi, Besiyeri, Hatice AYHAN, Nejat AYDIN, 1990, Etlik Veteriner Mikrobiyoloji Dergisi, S.aureus, S.epidermidis, Gox agar

Kaynakça

• AKAY Ö, OCAK, İ :, İZGÜR, M., ARDA, M., AYDIN, N., SULTAN, N. ve AYDIN, N. (1885) : insan ve ceşitli . kaynaklı ·stafilokok· suşlarının protein-A olusturmaları üzerinde bir araş tırma. A. Ü. Vet. Fak. 'Perg., 32: 401-402.
• CARRET, ·G .. · FLANDROIS;· J.P. and FLOUOURAT, J. (1981) : Detection of Staph .aureus protein-A by passive haemagglutination test using microtiter plate. Zbl. -Bakt. Hyg, 1. Abt. Orig. A., 249: 32-38 .
• ESSERS. L. and RADEBOLD, K. (1980) : Protain-A haemagglutination test a reliable method for rapid identification of staph . aureus . ·Hyg,. Abt. l. Orig.Reihe A .. 247: 170-176.
• FLANDROIS, J.P,. FLERETTE, J., et EYRAUD, F. (1976) : Detection de lo protein-A de Staph .aureus par hemagglutination conditionnee. · Ann. Biol. Clin .. 33: 3&5-3ô8.
• FORSGREN, A. ('197J) : Significance ·of protein -A production by Staphylococci.lnfect. lmmun., 2: 672,673.
• FORSGREN, A and SJÖQUIST, J. (.1967) : Protein-A from Staph .aureus. lll.Reaction with rabbit gamma globulin . J. lmmun . , 99: 19-24.
• iZGÜR, M .. AKAY, Ö. ve USLANOGLU, B. (1987) : insan ve hayvanlardan izole ve idenlifiye edilen S.aureus suşlarının protein-A oluşturmaları üzerine çeşitli besiyerlerinin etkisi. TÜBİTAK Proje No: VHAG-686.
• KRONVALL, G., HOLMBERG, O. and RIPA, T. (1972) : Protein-A in Staph. aureus strains of human and bovine origin. Acta Pathol. Microbiol. Scand. Sect. B .• 80: 735-742.
• LACHICA, V.F., GENIGERGIS, C.A. and HOEPRICH, P.D. (1979) : Occurence of protein-A in S.aureus and colsely related Staphylococcus species. J. Clin. Microbiol., 10: 752-753.
• LIND, 1. (1974) : Protein-A production in different strains of Staph.aureus under varied growth conditions. Acta Pathol. Microbiol. Scand., 82: 821·828.
• MAXIME, P.E ., MATHEWS, H.L. and MENGOLL, H.F. (1976) : Single tube mixed agglutination test for .the detection of stapylococcol protein-A. J. Clin. Microbiol., 4: 418-422.
• MULLER. H.P SCHAEG, W. and BLOBEL, H. (.1981) : Protein·A aktivitat von S.hyicus im vergleich zu protein A von S.areus. Zbl. Bakt. Hyg. ı. Abt. Orig. A., 249:_ 443-451.
• MULLER. -E. E:,- BRUCKLER, · J., SCHAEG, W. and BLOBEL (1973·) : Production of protein-A by cultures of Staph.aureus isolated from cattle and its pur(fication. Pesquisa Agropeccuaria Brasileirar, Serie Veterinaria., 8: 115· 119.
• NETER, E. and GORZYNSKI, E.A. (1959) : Studies on indlrect Staphylococcal :henıa.gglutination. Z. lnımun. Forsch., 118-288.
• OEDING. P. and HAUKENES, G. (1963) : ldentification of Staph . aureus antigens and antibodies by means of the gel precipitation teohnique. Acta Patihol. Microibiol. Scand. 57: 438-450.
• POUTREL, B. et LEFORT, B. (1980) : Mise au point et utilisation d'un rea- ctif sensible et staible · pour la detection de la proteine-A de Staph.aureus. Med. Maladies lnfect., 10: 38·41 .
• WEISS. R., SCHMEER, N., AMEND, A .. SCHAEG, W. and BEKHEAT. 1. (1984): Detection of pr.otein-A formatian by Staphylococci by means of ELISA. Zıbl.Vet. Med. B ., 31 : 424-434.
• WINBLAD. S. and ERICSON. C. (1973) : Sensitized sheep red cells a reactant for Staph.aureus protein·A. Acta Pathol. Microbiol. Scant. Sect. B.; .. 81: 150-156.