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«Establishment of the prevalance of persistently infected cows and sheep in Anatolia with FMDV»

Year 1993, Volume: 7 Issue: 4, 52 - 59, 01.12.1993

Abstract

For the purpose of the determination of the prevalance of the animals persistentiy
infected with FMDV, probang samples of 469 sheep and 147 cattle fomı
16 provinces in different districts of Anatolia have been collected. At the end
of the isolation carried out with primary bovine thyroid (BTY) cell cultures
and IB-RS 2 eell-line cultures and identification by CFT and/or ELISA the carrier
rate in sheep population has been detemıined 16.8 %and in cattle population
18.4% . The number of carrier animalsin Middle-East and South-East
Anatolia was more then the westren and northem parts. 4 sheep have been detennined
both A and O type FMDV carriers. It has been. confınned that BTY
cells were more susceptible to FMDV field samples than IB-RS2 cell-1ine.
Neverthe1ess, by taking both the disadvantages of BTY cell culture system and
the possible masking of FMDV partides by enteroviruses into consideration it
has been recommended to search and adapt other susceptible system to the laboratory.

References

  • BAILEY, N.J. : Statistical methods in biology. See. Ed. Edward Arnold of Hadder and Stoughton, (1981) London.
  • BENGIS. R.G., THOMSON. G.R., HEDGER R.S., DEVOS, V. and PIN!. A. (1986): FMD and the African buffalo. 1. carriers as a source of infection for cattle. Onderstepoort, J. vet. Res., 53, 69, 73,
  • CONDY, I. B .. R. S., HAMBUN, C. and BARNETT, I.T.R. (1985) : The duration of the FMDV carrier state in African Bufallo (i) in the individal animal and (ii) 'İn a free-living herd. Coınp. Imm. Microb. lngect. Dis., 8, 3 /4, 259-265.
  • DONALDSON. A.I., HERNIMAN. K.A.J. AND SELLERS, R.F. (1970) : Further investigations on the airborne exeretian of FMDV. J. Hyg .. com b., 68, 557-564.
  • DONALDSON. A.I., GIBSON. C.F., OLIVER. R., HAMBLIN, C., KITCHING, R.P. (1987): Infeelian of cattle by airborne FMDV : Minimal doses with Ol and SAT 2 strains. Res. Vet. Sci., 43, 339-346.
  • DONALDSON. A.l., KlTCHING. R.P. (1989): Tarsınisson of FMD by vaccinated caıtle fallawing natural challenge. Res. Vet. S ci., 46, 9-14.
  • FERRIS, N.P. AND DONALDSON, A.I. (1984): Comparative sensitivity of BTY cells and BHK 21 ce!ls for the innocuity testing of inactivated FMDV suspcnsions. Reunion FAD-Brescia, 26, 28, juin 1984.
  • GEERING, W.A. : FMDV, In Animal Health In Australia. vol. 9. Exotic diseases. Eds. W.A. Geering and A.J. Forman. Australian Govemıneni Publishing Service, 1987, 111-118.
  • HOUSE, C. and HOUSE, J.A . . (1989): Evaluation of tecnique to demonstrate FMDV in bovine tongue epithelium : Comparison of the sensitivity of cattle, mice, primary cell cultures, cryopreserved cell cultures and established cell-lines. Vet. Microb., 20, 99-109.
  • KITCHING, R.P., DONALDSON, A.I. (1987) : Calleetion and transportation of speciınens for vesicular virus investigation. Rev. Sci. Tech. off. Int. Epiz., 6-91, 263-272.
  • MOHANTY, G.C. and COTTRAL, G.E. (1971) : FMDV carrier state incells cultured from tissues of convalescent cattle. Are. ges.Virusforschung, 34, 1-13.
  • PACCIARINI, M., DESIMONE, F., FERRARI, S.: Detection of FMDV using PCR. Rep. Europ. com. cont. FMD. Res. Gr. Stand. Tech. Coınm., Lindholm, Denmark 25-29 june 1990, FAO, UN.
  • PAY, T.W.F. (1988) : FMD in sheep and goats. FMD Bul!., Coopers Animal Health Ltd., vol. 26, no. 3., 2-13.
  • Report of the Europ. Coınm. Cont. FMD. Res. Gr. Stand. Tech. Coının., Lindholın, Denmark 25-29 june 1990, FAO, UN.
  • SCHEMBRUCKER, C.G. and OULDRIDGE, E.J. : Immune repsonse ın calves vaccinated with FMD vaccine. Isı. Int. FMD Syınp., 1989, Ankara.
  • Scientific Report : The Animal Virus Research Institute Report for 1984-85. Coınmercial Press (Guildford) Ltd. 1986.
  • Scientific Report : The Aniınal Virus Research Institute Report for 1989. Taylor Bloxham Ltd. Leicester, 1990.
  • SNOWDEN, W. A. (1966) : Growth of FMDV in ınonolayer cultures of calf thyroid cells. Nature, 210, 1079-1080.
  • SUTTMOLLER, P. and Mc VICAR, W.J. (1968) : FMDV Carrier studies at the Pluınisland Aniınal Disease Laboratory. Europ. Comm. Cont. FMD Res. Gr. Stand. Tech. Coının ., Lindholm, Denınark , 18-20 Sep., 1968.
  • TORRE, J.C., DA VILLA, M., SOBRINO, F., ORTIN, J. and DOMINGO, E. (1985) : Establishment of cell-lines persistetly infected w ith FMDV. Virology, 145, 24-35.
  • TRUSTFIELD, M. Veterinary Epidemiology. Ed. M. Trustfield, Butterforth Pub. Ltd., 1986.
  • WITTMAN, G. :The virus carrier state in FMD (a summary). In Europ. Comm. Cont. FMD. Res. Gr. Stant. Tech. Comm., Lindholm, Denmark, 25-29 june, 1990.

Anadolu'da Şap ile Persiste Enfekte Sığır ve Koyunların Prevalansının Tesbiti

Year 1993, Volume: 7 Issue: 4, 52 - 59, 01.12.1993

Abstract

Şap virusu ile persiste enfekte hayvanların prevalansını tesbit etmek amacı ile Anadolu'da 16 ilden 469 koyun ve 147 sığır probang numunesi toplantı. Primer dana tiroid (BYT) ve IB-RS 2 devamlı hücre kültürlerinde gerçekleştirilen izolasyon ve CFT ve/veya ELISA ile gerçekleştirilen identifikasyon işlemleri sonucunda taşıyıcılık oranı koyunlar için %61,8 ve sığırlar için de %18,4 olarak belirlendi. Orta ve Güneydoğu Anadolu'daki taşıyıcı hayvan sayısı batı ve kuzey bölgelerine göre daha fazla idi. 4 koyunun hem A hem de O tipi şap virusu taşıyıcısı olduğu tesbit edildi. BTY hücrelerinin IB-RS 2 devamlı hücre kültürlerine oranla şap virusunun saha suşlarına daha duyarlı olduğu konusundaki önceki bulgular teyid edildi. Bununla beraber, BTY hücre kültürlerinin dezavantajları ve şap viruslarının hücre kültürlerinde enteroviruslar tarafından maskeleme olasılığı göz önüne alınarak yeni ve daha duyarlı test sistemlerinin laboratuvarlara adaptasyonu önerildi.

References

  • BAILEY, N.J. : Statistical methods in biology. See. Ed. Edward Arnold of Hadder and Stoughton, (1981) London.
  • BENGIS. R.G., THOMSON. G.R., HEDGER R.S., DEVOS, V. and PIN!. A. (1986): FMD and the African buffalo. 1. carriers as a source of infection for cattle. Onderstepoort, J. vet. Res., 53, 69, 73,
  • CONDY, I. B .. R. S., HAMBUN, C. and BARNETT, I.T.R. (1985) : The duration of the FMDV carrier state in African Bufallo (i) in the individal animal and (ii) 'İn a free-living herd. Coınp. Imm. Microb. lngect. Dis., 8, 3 /4, 259-265.
  • DONALDSON. A.I., HERNIMAN. K.A.J. AND SELLERS, R.F. (1970) : Further investigations on the airborne exeretian of FMDV. J. Hyg .. com b., 68, 557-564.
  • DONALDSON. A.I., GIBSON. C.F., OLIVER. R., HAMBLIN, C., KITCHING, R.P. (1987): Infeelian of cattle by airborne FMDV : Minimal doses with Ol and SAT 2 strains. Res. Vet. Sci., 43, 339-346.
  • DONALDSON. A.l., KlTCHING. R.P. (1989): Tarsınisson of FMD by vaccinated caıtle fallawing natural challenge. Res. Vet. S ci., 46, 9-14.
  • FERRIS, N.P. AND DONALDSON, A.I. (1984): Comparative sensitivity of BTY cells and BHK 21 ce!ls for the innocuity testing of inactivated FMDV suspcnsions. Reunion FAD-Brescia, 26, 28, juin 1984.
  • GEERING, W.A. : FMDV, In Animal Health In Australia. vol. 9. Exotic diseases. Eds. W.A. Geering and A.J. Forman. Australian Govemıneni Publishing Service, 1987, 111-118.
  • HOUSE, C. and HOUSE, J.A . . (1989): Evaluation of tecnique to demonstrate FMDV in bovine tongue epithelium : Comparison of the sensitivity of cattle, mice, primary cell cultures, cryopreserved cell cultures and established cell-lines. Vet. Microb., 20, 99-109.
  • KITCHING, R.P., DONALDSON, A.I. (1987) : Calleetion and transportation of speciınens for vesicular virus investigation. Rev. Sci. Tech. off. Int. Epiz., 6-91, 263-272.
  • MOHANTY, G.C. and COTTRAL, G.E. (1971) : FMDV carrier state incells cultured from tissues of convalescent cattle. Are. ges.Virusforschung, 34, 1-13.
  • PACCIARINI, M., DESIMONE, F., FERRARI, S.: Detection of FMDV using PCR. Rep. Europ. com. cont. FMD. Res. Gr. Stand. Tech. Coınm., Lindholm, Denmark 25-29 june 1990, FAO, UN.
  • PAY, T.W.F. (1988) : FMD in sheep and goats. FMD Bul!., Coopers Animal Health Ltd., vol. 26, no. 3., 2-13.
  • Report of the Europ. Coınm. Cont. FMD. Res. Gr. Stand. Tech. Coının., Lindholın, Denmark 25-29 june 1990, FAO, UN.
  • SCHEMBRUCKER, C.G. and OULDRIDGE, E.J. : Immune repsonse ın calves vaccinated with FMD vaccine. Isı. Int. FMD Syınp., 1989, Ankara.
  • Scientific Report : The Animal Virus Research Institute Report for 1984-85. Coınmercial Press (Guildford) Ltd. 1986.
  • Scientific Report : The Aniınal Virus Research Institute Report for 1989. Taylor Bloxham Ltd. Leicester, 1990.
  • SNOWDEN, W. A. (1966) : Growth of FMDV in ınonolayer cultures of calf thyroid cells. Nature, 210, 1079-1080.
  • SUTTMOLLER, P. and Mc VICAR, W.J. (1968) : FMDV Carrier studies at the Pluınisland Aniınal Disease Laboratory. Europ. Comm. Cont. FMD Res. Gr. Stand. Tech. Coının ., Lindholm, Denınark , 18-20 Sep., 1968.
  • TORRE, J.C., DA VILLA, M., SOBRINO, F., ORTIN, J. and DOMINGO, E. (1985) : Establishment of cell-lines persistetly infected w ith FMDV. Virology, 145, 24-35.
  • TRUSTFIELD, M. Veterinary Epidemiology. Ed. M. Trustfield, Butterforth Pub. Ltd., 1986.
  • WITTMAN, G. :The virus carrier state in FMD (a summary). In Europ. Comm. Cont. FMD. Res. Gr. Stant. Tech. Comm., Lindholm, Denmark, 25-29 june, 1990.
There are 22 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Original Article
Authors

S. İsmet Gürhan This is me

Burhan Gürhan This is me

Aysun Öztürkmen This is me

Gülhan Aynagöz This is me

Aysel Candaş This is me

Serdar Kızıl This is me

Publication Date December 1, 1993
Submission Date December 1, 1993
Published in Issue Year 1993 Volume: 7 Issue: 4

Cite

APA Gürhan, S. İ., Gürhan, B., Öztürkmen, A., Aynagöz, G., et al. (1993). Anadolu’da Şap ile Persiste Enfekte Sığır ve Koyunların Prevalansının Tesbiti. Etlik Veteriner Mikrobiyoloji Dergisi, 7(4), 52-59.

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