İNFEKSİYÖZ KERATOKUNJUNKTİVİTİS SEMPTOMLU SIĞIRLARDAN MOREXELLA BOVİS'İN İZALOSYONU VE KARAKTERİZASYONU

Year 1997, Volume: 9 Issue: 1, 89 - 93, 01.12.1997

Cengiz Çetin Taner Öncel Rabia Uçar Ömer Aydın

Abstract

Bu araştırmada, infeksiyöz keratokonjunktivitis semptomlu 92 sığırın 9'unda
Moraxella bovis saptandı. Sığırların hastalıklı gözlerinden alınan 107 örnekten,
11 M. bovis suşu izole edildi. İzole edilen suşların tümü besi yerinde düzgün
kenarlı yuvarlak, yarı şeffaf, grimsİ beyaz ve hafifçe besi yerine gömülmüş
koloniler oluşturdu . Subkültürde R tipi kolonilere hızlı bir değişim gözlendi.
İncelenen tüm M. bovis suşları Gram negatif diplobasil olarak görüldü. Sıvı
besiyerinde tüm suşlar hafif bulanıklık ve önemli derecede tortu oluşturarak
üredi. Tüm suşlar penisilin mevcudiyetinde hücresel uzama, oksidaz, katalaz,
20 °C' de üreme, tween 80, kazein, koagüle serum ve jelatİn hidrolizasyon testleri
yönünden pozitif, pigmentasyon, hareket, Mac Conkey agarda üreme, % 5 NaCl' e
tolerans, oksidasyon-fermentasyon (0-F), karbonhidrat fern1entasyon, indol,
hidrojen sülfid ve üre hidrolizasyon testleri yönünden negatifbul undu. İncelenen
suşların 8'i koyun kanlı agarda hemolitik bulunurken, 6 'sı nitrat redliksiyon
testinde pozitif sonuç verdi

Keywords

SIĞIR, SEMPTOM, MORAXELLA BOVİS, KERATOKONJUNKTİVİTİS, İZOLASYON, KARAKTERİZASYON

The isolation and characterization of Moraxella bovis from cattle with the symptoms of infectious keratoconjunctivis

Abstract

Moraxella bovis was detected in 9 out of 92 cattle with symptoms of
infectious keratoconjuntivitis. Eleven strains of M. Bovis were isolated from
107 sanıp i es obtained from affected eyes of cattele. Colonies of all strains isolated
were circular with a entire-edge, translucent, grayish-white and slightly indented
into the medium. In subculture, rapid dissociation to rough colonies was observed.
All M. bovis strains examined were appeared to be Gram-negative diplobacilli .
In liquid medium, all strains produced slight turbidity and considerable deposit
All strains produced positive results in the following tes ts: cellular elongation in
the presence penicilline, oxidase, catalase, growth at 20 °C, tween 80 and cascin
hydrolysis, coagulated senım and gelatine liquefaction. All stratins produced
negative results in the following tests: pigmentation, motility, growth on Mac
Conkey agar, tolerance to 5% NaCl, oxidation-fermentation (0-F),
carbonhydrates fermentation, indole and hydrogen sulphide production and urea
hydrolysis. OfM.bovis strains examined, eight were haemolytic on sheep blood
agar and six were positive in nitrate reduction test.

Keywords

Moraxella bovis, keratokonjunktivitis

References

• BAPTİSTA, P. J. H. P.(1979): lnfectious bovine keratoconjunctivitis: a review Br. Vet. J ., 135,225-242
• BRA YN, H. S., HEL PER, L. C., KILLIN GER, A. H., RHOADES, H . E. and MANSFIELD, M. E. ( 1973) : Soıne bacteriologic and ophthalmologic observations on bovine infectious keratoconjunctivitis in an lllinois beefherd. J.Am.Vet.Med. Assoc., 163,7,739,741.
• CATLİN, B. W. (1975) : Cellular elongation under the influence of antibacterial agents : Way to differentiatc coccobacilli from cocci . J.C1in . Microbiol. 1, 102-106.
• COWAN, S. T. and STEEL, K. J. (1974): Maımal forthe ldentifıcation of medical Bacteria p. 146-147 Cambridge Universty Prees, Cambridge. U.K.
• DEJA, 0., MÜ LLER W., BOCKLİSCH, H., STİEF, E., HEİNRİCH, B. and LAN GE., J. (1987) : Control of infectious bovine keratoconjunctivitis in young ca tt le rearing un it by means of a herd spesifıc Moraella bovis vaccine. Monat. Vet. 42,14,501 -505.
• ERDEGER, J and AYDIN, N. (1991): Sığırlardan izoleedilen Moraxella bovis suşlarının çeşitli özelliklerinin araştırılması. Doğa Türk Vet. Hayv. Derg. 15,2, 140-147.
• FAULL, W, B. and HAWKSLEY, M. B. (1954): Infectious keratitis in cattle associated with Moraxella bovis. Vet. Rec. 66,22,311-312.
• FRASER, M. C., BERGERON, J. A., MAYS, A. and AİELLO, S ( 1991) : lnfectious keratoconjunctivitis: The M erek Veterinary Manual. 7 th. ed, p. 305-306 Merck Co., Ine. Rahway N.J.,USA.
• KODJO, A., MOUSSA, A., BORGES, E. and RİCHARD, Y. ( 1993): Identifıcation of Moraxella-like bacteria isolatcd from caprine and ovine nasal flora: J.Vet.Mcd Series B 40, 97-104 .
• MATTİNSON, A. D. and COX P. J. (1982): Use oftwcen 80 agar in the study of Moraxella bovis infection in cattle,: Vet. Rec. 111 ,395-396 ..
• PUGH, G. W., HUGHES, D. E and McDONALD, T. J. ( 1966): The isolation and charactcrization of Moraxella bovis. Am.,J. Vet. Res. 27, 119 , 957-962.
• PUNCH, P. 1 and SLATTER, D. H. (1984): A review ofinfectious bovine kcratoconjunctivitis. : Vet. Bul. 54. 4, 193-207.
• REESE, W. C. ( 1967): Treatment ofbovine keratoconjunctivitis with furazolldonc: Vet. Med . Smail Anim. C lin. 62,999-1002.
• SPRADBROW, P. B. ( 1967) : A microbiological study of bovine conjunctivitis and keratoconjunctivitis .: Aust. Vet. J. 43 . 55-58.
• WiLCOX, G.E.( 1970) : An cxamination of Moraxclla reıated gencra coınınonly isolated from the bovine eye . J. Coınp . Path. 80, 65-74 . WiLCOX, G. E.( 1970) The aetiology of infectious bovine keratoconjunctivitis in Queensland. : Au st. Vct.J .46,415-420.