Leptospirozisin Klinik Mikrobiyolojik Tanısı ve Son Gelişmeler

Yıl 2003, Cilt: 14 Sayı: 1-2, 64 - 73, 01.12.2003

A. Levent Kocabıyık Cengiz Çetin

Öz

Leptospirozis, evcil ve vahşi hayvanlar ile insanlarda Leptospira interrogans serovadarının neden olduğu,
dünya çapında yaygınlık gösteren zoonoz bir enfeksiyonduı: Hastalık abort, yaşama gücü zayıf buzağı doğumu, süt
v eriminde düşme ve infertilite nedeniyle önemli ekonomik kayıplara neden olmaktadıı: Leptospirozis, geniş spektrumlu
semptomları ve klinik bulguları nedeniyle birçok bakteriyel, viral ve paraziter hastalıklada benzerlik göstermekte,
bu nedenle de klinik mikrobiyolojik tanı enfeksiyonun kesin teşhisinde tek çıkar yol olmaktadı ı: Bu derleme de,
kültür, direkt mikroskopik muayene, moleküleı- ve serolojik metotlar gibi klinik mikrobiyolojik tanı metotları son
gelişmeler ile birlikte sunulmuştur.

Anahtar Kelimeler

Leptospirosis, klinik mikrobiyolojik tanı, A. Levent KOCABIYIK, Cengiz ÇETİN, 2003, Etlik Veteriner Mikrobiyoloji Dergisi

Clinical Microbiological Diagnosis of Leptospirosis and the Last Improvements

Öz

Leptospirosis is a world-wide zoonotic infection that ajfects domestic and wild anima/s and humans caused by
serovaı·s of Leptospira interı-ogans. Disease couses ekonomic loses due to abortion, stillbirth, biı-th of premature and
weak infected calves, infertility, mastitis, reduced milk production and death causing important financial losses in
cattle bre ed ing. Due to these broad spectrum of symptoms and elinical sin gs, the disease resembles to other bacterial,
viral, and parasitic diseases so that elinical micı-obiological diagnosis is comideı-ed to be sol e way for exact diagnosis.
In this review artiele, elinical microbiological diagnostic methods ineluding culture, direct microscopic examination,
moleculaı· and serological methods aı·e presented with the last improvements.

Anahtar Kelimeler

A. Levent KOCABIYIK, Cengiz ÇETİN, Leptospirosis

Kaynakça

• (2002). Evaluation of eight rapid screening tests for acute leptospirosis in Hawaii. J Clin Microbiol, 40, 1464-1469.
• EFFLER PV, DOMEN HY, BRAGG SL, AYET, SASAKI DM (2000). Evaluation of the indirect hemagglutination ass ay for diagnosis of acute leptospirosis in Hawaii. J Clin Microbiol, 38, 1081-1084.
• FAINE S, ADLER B, BOLIN C, PEROLAT P (1999). Leptospira and Leptospirosis, 2nd edition, MediSci, Melbourne, Australia.
• GUSSENHOVEN GC, VAN DER HOORN MA, GORIS MG, TERPSTRA WJ, HARTSKEERL RA, MOL BW, VAN INGEN CW, SMITS HL (1997). LEPTO dipstick, a dipstick assay for detection of Leptospim-specific immunoglobulin M antibodiesin human sera.] Clin Microbiol, 35,92-97.
• HARTSKEERL RA, SMITS HL, KORVER H, GORIS MGA, TERPSTRA WJ (2002). Handbook of International Course on Laboratory Methods for the Diagnosis of Leptospirosis, Royal Tropical Institute, Amsterdam, The Netherlands.
• KOCABIYlK AL, CETIN C (2003). Detection of Leptospira interrogans seravar hardjo antibodies by the microscopic agglutination test and enzyme-linked immunosorbent assay in cattle seı·a. Inciian Vet J, 80, 969-971.
• KRAMER KJ, PANG LW, MINETTE HP, PERRONE JB (1994). Evaluation of the quantitative bıif!Y coat analysis (QBC) system for the detection of Leptospira in human blood. Southeast Asian J Trop Med Public Health, 25,788-789.
• LEVETT PN (2001). Leptospirosis. Clin Microbiol Rev, 14,296-326.
• LEVETT PN, BRANCH SL, WHITTINGTON CU, EDWARDS CN, FAXTON H (2001). Two methodsfor rapid semlogical diagnosis of acute leptospirosis. Clin Diagn Lah lmmunol, 8, 349-351.
• LEVETT PN, WHITTINGTON CU (1998). Evaluation of the indireel hemagglutination assay for diagnosis of acute leptospirosis. J Clin Microbiol, 36, 11-14.
• MATTHIAS MA (2000). Malecular characterization of pathogenic Leptospira from anima/s on the isiand of Barbados, Ph.D. thesis, University ofWest lndies, Barbados.
• MERIEN F, BARANTON G, PEROLAT P ( 199 5). Comparison of polymerase chain reaction with microagglutination test and culture for diagnosis of leptospirosis. J lnfect D is, 172, 281- 285.
• MYERS DM (1987). Seı-odiagnosis of human leptospirosis by counterimmunoelectrophoresis. J Clin Microbiol, 25, 897-899.
• PLANKR,DEAND (2000). Overviewofthe epidemiology, microbiology, and pathogenesis of Leptospira spp. in humans. Microbes lnfect, 2, 1265-1276.
• RAMADASS P, SAMUEL B, NACHIMUTHU K (1999).A rapid latex agglutination test for detection of leptospiral antibodies. Vet Microbiol, 70, 137-140.
• ROMERO EC, BILLERBECK AEC, LANDO VS, CAMARGO ED, SOUZA CC, YASUDA PH (1998). Detection of Leptospira DNA in patients with aseptic meningilis by PCR.J Clin Microbiol, 36,1453-1455.
• SEHGAL SC, VIIAVACHARI P, SUBRAMANlAM V (1997). Evaluation of leptospiı·a micro capsule agglutination test (MCAT) for seradiagnosis of leptospirosis. Inciian J Med Res, 106, 504-507.
• SILVA MV, NAKAMURA ·PM, CAMARGO ED, BATISTA L, VAZ AJ, ROMERO EC, BRANDAO AP (1997). Immunodiagnosis of human leptospirosis by dotELISA for the detection of IgM, IgG, and IgA antibodies. Am] Trop Med Hyg, 19,485-492.
• SIL VA MV, CAMARGO ED, BATISTA L, VAZ AJ, FERREIRA AW, BARBOSA P.R.S (1996). Application of anti-leptospiı-a ELISA-IgM for the etiologic elucidation of meningitis. Rev lnst Med Trop Sao Paulo, 38, 153-156.
• SMITH CR, KETTERER PJ, MCGOWAN MR, CORNEY BG (1994). A ı·eview of laboratoıy techniques and their use in the diagnosis of Leptospiı-a interrogans seravar hardjo infection in cattle. Aust Vet J, 71, 290-294.
• SMITS HL, CHEE HD, EAPEN CK, KURIAKOSE M, SUGATHAN S, GASEM MH, YERSIN C, SAKASI D, LAI-A-FAT RF, HARTSKEERL RA, LIESDEK B, ABDOEL TH, GORIS MG, GUSSENHOVEN GC (2001). Latex basecl, ı-ap id and easy ass ay for human leptospirosis in a single test format. Trop Med Int Health, 6, 114-118.
• SMITS HL, VAN DER HOORN MA, GORIS MG, GUSSENHOVEN GC, YERSIN C, SASAKI DM, TERPSTRA WJ, HARTSKEERL WJ (2000). Simple latex agglutination assay for rapid seradiagnosis of human leptospirosis. J Clin Microbiol, 38, 1272-1275.
• SMITS HL, ANANYlNA YV, CHERESHSKY A, DANCEL L, LAI-A-FAT RF, CHEE HD, LEVETT PN, MASUZAWAT, YANAGIRARA Y, MUTHUSETHUPATHI MA, SANDERS EJ, SASAKI DM, DOMEN H, YERSIN C, AYET, BRAGG SL, GUSSENHOVEN GC, GORIS MG, TERPSTRA WJ, HARTSKEERL RA (1999). International multicenter evaluation of the elinical utility of a dipstick assay for detection of Leptospiı-a-specific immunoglobulin M antibodies in human serum specimens. J Clin Microbiol, 37,2904-2909.
• TAYLORMJ,ELLISWA,MONTGOMERY JM, YAN KT, MCDOWELL SW, MACKIE DP (1997). Magnetic immunocapture PCR assay (MIPA): detection of Leptospiı-a borgpetersenii seravar hardjo. Vet Microbiol, 56, 135-145.
• WAGENAAR ], ZUERNER RL, ALT D, BO LIN CA (2000). Comparison of polymerase chain reaction assays with bacteriologic culture, immunojluoressence, and nucleic acid hybridization for detection of Leptospiı-a borgpetersenii serovaı· hardjo in uı·ine of cattle. Am J Vet Res, 61, 316-320.
• WOO THS, PATEL BKC, SMYTHE LD, NORRIS MA, SYMONDS ML, DOHNT MF (1998). Identification of pathogenic Leptospira by TaqMan probe in a lightcycleı·. Anal Biochem, 256. 132-134.
• WOODWARD MJ, SWALLOW C, KlTCHING A, DALLEY C, SAYERS AR ( 1997). Leptospira haı·djo serodiagnosis: a comparison of MAT, ELISA and immunocomb. Vet Rec, 141, 603-604.
• YAN KT, ELLIS WA, MONTGOMERY JM, TAYLOR MJ, MACKIE DP, MCDOWELL SW (1998). Development of an immunomagnetic antigen capture system for detecting leptospires in bovine urine. Res Vet S ci, 64, 119-124