Kuduz Hastalığının Teşhisinde Polimeraz Zincir Reaksiyonu

Yıl 2004, Cilt: 15 Sayı: 1-2, 1 - 14, 01.12.2004

Hikmet Ün Feray Alkan

Öz

Bu çalışmada kuduz hastalığının teşhisinde Polimeraz Zincir Reaksiyonu (PCR) tekniğinin
kullanım olanakların araştırılması amaçlandı . Toplanan materyallerin Floresan Antikor Tekniği
(FAT) ile kontrolü sonuçları heminested RT-PCR teslinden elde edilen sonuçlar ile karşılaştırıldı.
Ayrıca kokuşmuş materyallerden heminested RT-PCR ile kuduz hastalığının teşhisi olanaği araştırıIdi.
Çalişmada 83 adedi FAT ile değerlendirilebifen ve 30 adedi de kokuşmuş olmaları nedeni ile
FAT ile değerlendirilemeyen toplam 113 adet değişik hayvan türüne ait beyin örneği kullanıIdi.
FAT ile değerlendirilebilen 83 materyalden 80 adedi kuduz yönünden müspet. 3 adedi ise kuduz yönünden menfi idi. Bu materyaller Tarım ve Köyişleri Bakanfığma bağlı kuduz teşhis
lahoratuvarından temin edildi.
FAT ile müspel değerlendirilen 80 materyalden 49 (% 61,25) adedinde RT-PCR ile viral
nükleik asit tespit edilirken, heminested RT-PCR ile tamamında (% 1 00) viral nükleik asit saptandı.
FAT ile menfi olan 3 adet materyal de RT-PCR ve heminested RT-PCR ile de viral nükleik asit tespit
edilmedi. 30 adet kokuşmuş materyalden 4 adedinde RT-PCR ile viral nükleik asit (%13.33) ve 6
adedinde de heminested RT-PCR .ile viral nükleik asit (% 20) tespit edildi. Heminested RT-PCR ile
viral nükleik asit tespit edilen bu 6 materyalden 3 adedi istanbul Pendik Veteriner Kontrol ve Araştırma
Enstitüsünden temin edilen, diğer 3 adedi de Etlik Merkez Veteriner Kontrol ve Araştırma
Enstitüsünde aşı üretimi ve deneysel çalişmalarda kullanılan hayvanlara ait beyinlerdi. Deneysel amoçlarla kullanılanlar ve kuduz virusu ile enfekte edilmiş olan hayvanlar (3 adet) hariç tutulacak
olursa kokuşmuş materyallerdeki RT-PCR ve heminested RT-PCR pozitiflik oranları sırası ile % 3. 7
( 1/2 7) ve% 11.11 (3/2 7) olarak belirlendi.

Anahtar Kelimeler

Teşhis, Kuduz, RT-PCR, Heminested RT-PCR

Polymerase C hain Reaction in the Diagnosis of Rabies

Öz

In this study the probability of the use of the PCR technique for the diagnosis of rabies was
investigated. The colected specimens were examined using the FAT and the results were compared
with the ones thal were obtained from the heminested RT-PCR test. Furthermore. it was investigated
the diagnosis of rabies in the decomposed specimens using the heminesled RT-PCR.
In this study. 83 brain specimens to evaluate using FAT and 30 decomposed materials not to
evatluate using FAT belonging to various animal species totally 113 brain pecimens were used. 80
of these specimens were found to be positive using FAT while 3 of them being negative using FAT
All of these specimens were obtained from the rabies diagnosis laboratories belonging to the Ministry
of Agriculture and Rural Affairs.
Among the 80 specimens that were found to be positive using FAT, 49 of them were determined
to be positive using RT-PCR (61,25%) while all of these 80 specimens were found to be pasitive using heminesled RT-PCR (100 %). The 3 specimens that were found to be negative using FAT
all of them were found to be negative using RT-PCR and heminested RT-PCR. 4 of the 30 decomposed
brain specimens were determined to be positive (13,33%) us ing RT-PCR and 6 of them found
to be positive using heminested RT-PCR. If the results of the brain specimens that have been used
for experimental purposes and animals that have been injected with rabies virus (3 animals) are excfuded,
these rates are 3. 7% and 11, 11I% respeclively in the decomposed materials. Among the 6
decomposed specimens were found to be positive using heminested RT-PCR, 3 of them were obtained.
from the İstanbul Pendik Veterinary Control and Research Institute, the other 3 of them were
usedfor experimental pwposes and vaccine production in Etlik Central Veterinary Control and Research
Institute.

Anahtar Kelimeler

Rabies, RT-PCR, Heminested RT-PCR, Diagnosis., Diagnosis

Kaynakça

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