Türkiye'de Üretilen Ppr Aşısının Bagışıklık Süresinin Tespiti

Yıl 2006, Cilt: 17 Sayı: 1-2, 13 - 22, 01.12.2006

Nigar Tatar Özden Kabaklı

Öz

Bu çalışma, Türkiye' de ilk defa üretimi gerçek-
leştirilen PPR aşısı ile yapılan aşılamadan sonra
koyun ve keçilerde bağışıklığın süresi hakkında
yeterli verilerin sağlanması amacıyla gerçekleşti-
rildi. Etlik Merkez Veteriner Kontrol ve Araştırma
Enstitüsü' nde (EMVKAE) üretilen attenüe PPR
aşısının ilk dört serisi (PPR 0277-1, PPR 0277-2,
PPR 0277-3, PPR 0277-4) ile koyun ve keçi sürü-
lerinde dört farklı grupta deneysel ve saha çalış-
ması yapıldı. Aşılanan hayvanlarda bağışıklık
seviyesi Virus Nötralizasyon Test (VNT) ve PPR
C-ELISA ile gözlendi.
Birinci deneysel çalışma grubunda; deneme ahırlarındaki aşılı 22 koyun ve keçide (11 adet koyun ve 11 adet keçi ) ilk üç seri PPR aşısı ile (PPR 0277 -1, PPR 0277-2, PPR 0277-3 ) aşılama sonrası bağışıklık tespiti için çalışıldı. Aşı sonrası seroprevalans her iki türde de %100 bulundu. 2. yılın sonunda aşılı hayvanların hepsinde koruyucu düzeyde (>1:100) antikor titresi tespit edildi.
İkinci saha deneysel çalışma grubunda; Burdur'da aşılama öncesi PPRV antikorları yönünden seronegatif olan sürüde, 46 adet koyun ve 47 adet keçi ile çalışıldı. Aşılamadan bir yıl sonra her iki türde de aşılı hayvanlar %100 seropozitif bulundu. I. ve II. grupta oluşabilecek bir PPR enfeksiyonun tespiti amacıyla bırakılan aşısız kontrol gruplar ise çalışma süresince seronegatif olarak saptandı.
,İlk 3 seri PPR aşısının kullanıldığı Antalya, Manisa ve Mardin de 972 adet koyun ve keçinin örneklendiği saha çalışması yapıldı. Aşılamadan bir yıl sonra aşılı olduğu bildirilen toplam 742 hayvanın %78'i PPR seropozitif bulundu. Farklı 3 seri ile aşılanan sürülerdeki seropozitiflik oranı ise sırasıyla; PPR 0277-01 serisinde %70, PPR 0277-2 serisinde %75, PPR 0277-3 serisinde %95 olarak tespit edildi. Sahada bulaşmadan şüpheli hayvanlara da aşı uygulandığı ve bu sürülerde ki bağışıklık oranının sirayete maruz hayvanlardan daha düşük olduğu tespit edildi.
Deneme ahırlarında ki aşılı gebe keçilerden doğan 16 oğlakta meternal bağışıklık süresi 4-7 ay arasında tespit edilmekle birlikte 16 oğlaktan 13 adetinde maternal bağışıklık süresi 4-5 ay arasında tespit edildi. İlk doğdukları günlerdeki yüksek seviyedeki antikor titreleri ikinci ayın sonunda daha düşük seviyelere indi. Aşısız kontrol analardan doğan oğlaklar ise çalışma süresinde seronegatif bulundu.
Bu çalışmada PPR aşılama sonraı bağışıklık süresinin her iki türde de 2 yıldan daha uzun olduğu tespit edildi. Maternal bağılıkşık süresinin ise 4-5 ay sonra yapılmasının uygun olduğu ve sürü dinamiği ve sahada bulaşmadan şüpheli hayvanların da aşılanmış olabilceği göz önünde bulundurularak yıllık tekrar yapılmasının sürü bağışıklığı açısından gerekli olduğu sonucuna varıldı.

Anahtar Kelimeler

PPRV, Vaccine, İmmunitiy, PPR, Bağışıklık, Nigar TATAR, Özden KABAKLI, 2006, Etlik Veteriner Mikrobiyoloji Dergisi

Detection the duration of immunity by PPR vaccine produced in Turkey

Öz

This study was conducted to obtain enough
data on immunity period of PPR vaccine which
was produced in Turkey for the first time in sheep
and goats. Experimental and field studies· were
conducted in four different groups of sheep and
goat herds with the first four series (PPR 0277-1 ,
PPR 0277-2, PPR 0277-3, PPR 0277-4) ofhomologous
attenuated PPR vaccine produced in Etlik
Central Veterinary Control and Research Im;titute.
Immunity levels of vaccinated animals were
determined by Virus Neutralisation Test and by
PPR C-ELISA.
In the first experimental study group; immunity
levels of 22 sheep and go ats (ll sheep and ll
goats) were studied which were vaccinated with
the first three series (PPR 0277-1, PPR 0277-2,
PPR 0277-3) of PPR vaccine at the experimental
animal un it of the Institute. Postvaccination sero-
prevalance was found a~ 1 00% in both species.
Protective antibody titers (> : 10) were determined
in all vaccinated animals at the end of the second
year.
determined that animals which were suspected of
being infected were also vaccinated and within
these herds immunity levels were lower compared
to those of Although matemal immunity period of 16 kids
bom from vacinated goats in experimentalanimal
units were found between 4-7 months, the maternal
imrnunity period of 13 of these 16 goats were
found between 4-5 months. High antibody titres
detected in the firs! few days following birth were
decreased at the en d of the second month. The kids
which were bom from unvaccinated control goats
were found as seronegative throughout the study.
In this study, the immunity period following
PPR vaccine was found as longer than two years
in both species.

Anahtar Kelimeler

PPRV, vaccine, immunity

Kaynakça

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