Liyofilize Attenue Koyun- Keçi Vebası Aşısı Raf Ömrünün Belirlenmesi

Yıl 2006, Cilt: 17 Sayı: 1-2, 23 - 28, 01.12.2006

Özden Kabaklı İbrahim Hancı Süreyya Yöndem

Öz

Bu çalışma Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü tarafından üretilen liyofilize attenue PPR (koyun- keçi vebası) aşısının -20°C ve +4 (+2/+8) oc dereceler arasındaki raf ömrünün saptanması amacıyla yapıldı. Araştırmada Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü tarafından üretilen PPR 0277-4, PPR 0277-4A, PPR 0377-5 seri nolu aşıların raf ömürleri 24 ay süreyle kontrol edildi. Aşıların titreleri liyofilizasyon tarihlerinde PPR 0277-4 ve PPR 0277-4A seri nolu aşılarda ıo-6.4 - ıo-6 ·3 DKID50/ml olarak bulunur iken +4 (+2/+8) oc derecede depolanan aşılarda 24. ayda titrenin ıo-6 · DKID50/ml'e düştüğü , PPR 0377-5 seri nolu aşılarda 1 o-6 8 DKID50/ml olarak bulunur iken 24. ayda titrenin ıo-6 ·5 DKID50/ml'e düştüğü saptandı fakat bu düşüş istatistiki açıdan önemli bulun- madı. Aşıların -20°C de bekletilmesiyle titrelerinde değişiklik olmadığı belirlendi. Bu çalışma ile liyofilize attenue PPR (koyun-keçi vebası) aşısının -20°C ve +4 ( + 2/+8)°C dereceler arasındaki raf ömrünün 2 yıla (24 aya) çıkarılmasının uygun olacağı sonucuna varılmıştır.

Anahtar Kelimeler

Koyun, Keçi, Veba, Aşı, Özden KABAKLI, ibrahim HANCI, Süreyya YÖNDEM, 2006, Etlik Veteriner Mikrobiyoloji Dergisi, Koyun Keçi Veba Aşısı

"Determination of shelf- life of Peste des petits ruminants vaccine"

Öz

The aim of this study w as to determine the selflife of lyophilized attenue PPR vaccine (Sheep and Go at Plague) produced by Etlik Central Veterinary Control and Research Institute at between -20 °C and +4 (+2/+8) oc. At the study PPR 0277-4, PPR 0277-4A and PPR 0377-5 seri- al numbered vaccines had been controlled for 24 months. Whereas the titres of PPR 0277-4 and PPR 0277-4A serial numbered vaccines were deter mined as 1 o-6.4 - 1 o-6· 3 TCID50/ml at the lyophilized time, it was noticed that the titres were decreased to ıo-6· ı TCID50/ml after 24 months storage period at +4 (+2/+8) C. While the titre ofPPR 0377-5 serial numbered vaccines show ed 1 o-6· 8 TCID50/ml at the lyophilized time, it was decreased to 1 o-6 ·5 TCID50/ml after 24 months but it was found no significant statistically. It was noticed that there was no changes at the titres of the vaccines with storing them at -20 oc . According to the result of this study it will be can venient to extend the shelf-life of the PPR vaccine up to 24 months at -20 oc ve +4 (+2/+8) oc

Anahtar Kelimeler

PPR, Vaccine, Shelf-life

Kaynakça

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