Araştırma Makalesi
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Detection of bovine Partuberculosis with ELISA and fecal examination

Yıl 2014, Cilt: 25 Sayı: 1, 1 - 5, 01.12.2014

A.ebru Borum Sertan Çatık Zafer Mecitoğlu Gülşah Demir Mihriban Ülgen Sezgin Şentürk

Öz

In this study, 305 between 4-8 years on a farm in Holstein Fresian dairy cows in Afyon were used. In two of
these animals with chronic diarrhea were present and cows did not respond to treatment. Clinical studies, hematological
parameters were determined, and blood serum and fecal samples were taken in other cattle in the herd. Mycobacterium
avium subsp. Paratuberculosis (MAP) in serum samples was determined using ELISA. The prevalence of paratuberculosis
in dairy cows, Ziehl-Neelsen staining with 4.59% in fecal samples, 31.80% positive by the ELISA test were
determined.

Anahtar Kelimeler

ELISA Paratuberculosis Ziehl Neelsen Staining

Kaynakça

  • Abendan N, Sevilla IA, Prieto JM , Garrido JM, Juste RA, Alonso-Hearn M, (2013). Mycobacterium aviumsubspeciesparatuberculosis isolates from sheep and goats Show reduced persistence in bovine macrophages than cattle,bison, deer and wild boar strains regardless of genotype. Vet Microbiol. 163, 325-334.
  • Antognoli MC, Hirst HL, Garry FB, Salman MD, (2007). Immune Response to and Faecal Shedding of Mycobacterium avium ssp. paratuberculosis in Young Dairy Calves, and the Association Between Test Results in the Calves and the Infection Status of their Dams. Zoonoses Public Hlth . 54, 152-159.
  • Collins MT, and Sockett DC, (1993a). Accuracy and economics of USDA-Licensed enzyme-linked immunosorbent assay for bovine paratuberculosis. J Am Vet Med Assoc. 203, 1456-1463.
  • Collins MT, Sockett DC, Ridge F, (1993b). Evaluation of a commercial enzyme-linked immunosorbent assay for Johne’s disease. J Clin Microbiol. 5, 52-55.
  • Collins MT, Gardner IA, Garry FB, Roussel AJ, Wells JS, (2006). Consensus recommendations on diagnostic testing for the detection of paratuberculosis in cattle in the United States. JAVMA-J AM VET MED A 229(12), 1912-1919.
  • Çetinkaya B, Erdoğan HM, Morgan KL, (1997). Risk factors for Bovine Paratuberculosis. II. The multiple analysis of risk ractors for Bovine Paratuberculosis. Turk J Vet Anim Sci. 21, 303-306.
  • Çetinkaya B, Muz A, Ertaş HB, Öngör H, Sezen İY, Gülcü HB, (2000). Süt ineklerinde Paratüberküloz Prevalansının Polimeraz Zincir Reaksiyonu (PZR) ile Saptanması. Turk J Vet Anim Sci. 24, 371-379.
  • De Kantor IN, Weyer KE, (2004). 1998-last update,laboratory services in tuberculosis control, part II.
  • Dieguez FJ, Gonzalez AM, Menendez S, Vilara MJ, Sanjuana ML, Yus E, Arnai I, (2009). Evaluation of four commercial serum ELISAs for detection of Mycobacterium avium subsp. paratuberculosis infection in dairy cows. Vet J. 180(2), 2231-235.
  • Fujiki A, (2001). Direct smear examination. In: TB Bacteriology Examination to Stop TB. The Research Institute of Tuberculosis, ed. Fujiki A, Japan International Cooperation Agency JINNOU Co. 7.
  • Garcia RR, Perez-de-la-Lastra JM, Vicente J, Ruiz- Fons F, Garrido JM, Gortazar C, (2008). Large-scale ELISA testing of Spanish red deer for paratuberculosis. Vet Immunol Immunopathol.124, 75-81.
  • Gasteiner J, Wenzli H, Fuchs K, Jark U, Baumgartner W, (1999). Serological Cross-sectional Study of Paratuberculosis in Cattle in Austria . J Vet Med. B 46, 457-466.
  • Gilardoni LR, Paolıcchi FA, Mundo SL. (2012). Bovine paratuberculosis: a review of the advantages and disadvantages of different diagnostic tests. Rev Argent Microbiol. 44, 201-215.
  • Lambeth C, Reddacliff LA, Windsor P, Abbott KA, MacGregor H, Whittington RJ, (2004). Intrauterin and transmammary transmission of Mycobacterium avium subsp. paratuberculosis in sheep. Aust Vet J. 82, 504-508.
  • Makav M, Gökçe E, (2013). Kars Yöresi Sığırlarında Subklinik Paratuberkülozun Seroprevalansı. Kafkas Univ Vet Fak Derg. 19 (5), 913-916.
  • Mura M, Bull TJ, Evans H, Sidi-Boumedine K, McMinn L, Rhodes G, Pickup R, Hermon-Taylor J, (2006). Replication and long-term persistence of bovine and human strains of Mycobacterium avium subsp. paratuberculosis within Acanthamoeba polyphaga. Applied Environ Microbiol 72, 854-859.
  • Naser SA, Ghobrial G, Romero C, Valentine JF, (2004). Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn’s disease. Lancet 364, 1039-1044.
  • Nielsen SS, Toft N, (2008a). Ante mortem diagnosis of paratuberculosis: A review of accuracies of ELISA, interferon-ɣ assay and faecal culture techniques. Vet Microbiol. 129, 217-235.
  • Nielsen SS, (2008b). Transitions in diagnostic tests used for detection of Mycobacterium avium subsp. paratuberculosis. Vet Microbiol. 132, 274-282.
  • Öztürk D, Pehlivanoğlu F, Tok AA, Günlü S, Güldali Y, Türütoğlu H, (2010). Seroprevalence of paratuberculosis in the Burdur province (Turkey), in dairy cattle using the enzyme linked immunosorbent assay (ELISA). Israel J Vet Med. 65, 53-57.
  • Paolicchi A, Zumarraga MJ, Gioffre A., Zamorano P, Morsella C, Verna1A, Cataldi A, Alito A, Romano M, (2003). Application of Different Methods for the Diagnosis of Paratuberculosis in a Dairy Cattle Herd in Argentina. J Vet Med. B 50, 20-26.
  • Rajukumar K, Tripathi BN, Kurade NP, Parihar NS, (2001). An Enzyme -liınked Immunosorbent Assay using Immuno affinity-purified Antigen in the Diagnosis of Caprine Paratuberculosis and Its Comparision with Conventional ELISA. Vet Res Commun. 25, 539-553.
  • Senturk S, Metcitoglu Z, Ulgen M, Borum E, Temizel E, Kasap S, (2009). Evaluation of serum iron and iron binding capacity in cows with paratuberculosis Tierarztl Prax. 6,375-378.
  • Shahmoradi AH, Arefpajohi Reza, Tadayon K, Mosavari N, (2008). Paratuberculosis in Holstein-Friesian cattle farms in Central Iran Trop Anim Health Prod 40, 169-173.
  • Shin SJ, Cho D, Collins MT, (2008). Diagnosis of bovine paratuberculosis by a novel enzyme-linked imunosorbent assay based on early secreted antigens of Mycobacterium avium subsp. paratuberculosis. Clin Vaccine Immunol, 15, 1277-1281.
  • Singh SV, Singh AV, Singh R, Sharma S, Shuklab N, Misraa S, Singh PK, Sohala JS, Kumar H, Patil PK, Misrad P, Sandhu KS, (2008). Sero-prevalence of Bovine Johne’s disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis ‘Bison type’ genotype of goat origin. Comp Immunol Microb. 31, 419-433.
  • Stabel, JR, (1996). Production of gamma-interferon by peripheral blood mononuclear cells: an important diagnostic tool for detection of subclinical paratuberculosis. J Vet Diagn Invest 8, 345-350.
  • Sweeney RW, Whitlock RH, Rosenberger A, (1992). Mycobacterium avium subsp. paratuberculosis cultured from milk and suprammamary lymph nodes of infected asyntomatic cows. J Clin Microbiol. 30, 166-71.
  • Uzoigwe JC, Khaitsa ML, Gibbs PS, (2007). Epidemiological evidence for Mycobacterium avium subspecies paratuberculosis as a cause of Crohn’s disease. Epidemiol Infect. 135, 1057-1068.
  • Yıldırım D, Civelek T, (2013). Prevalence of subclinical paratuberculosis in dairy cattle in Uşak Region, Kars Yöresi Sığırlarında Subklinik Paratuberkülozun Seroprevalansı Kafkas Univ Vet Fak Derg. 19 (1), 121-126.
  • Yooa HS, Shin SJ, (2012). Recent research on bovine paratuberculosis in South Korea. Vet Immunol Immunopathol.148, 23-28.

ELISA ve fekal bakteriyoskopi ile sığırlarda Paratuberküloz prevalansının belirlenmesi

Yıl 2014, Cilt: 25 Sayı: 1, 1 - 5, 01.12.2014

A.ebru Borum Sertan Çatık Zafer Mecitoğlu Gülşah Demir Mihriban Ülgen Sezgin Şentürk

Öz

Bu çalışmada Afyon ve civarındaki çiftliklerden 4-8 yaş arasındaki 305 Holstein Fresian sütçü sığırlar kullanılmıştır.
Bu sığırların iki tanesinde kronik diare şikayeti mevcuttu ve yapılan tedaviye cevap vermemiştir. Sürüdeki diğer
sığırlarda klinik inceleme yapılmış, hematolojik parametrelere bakılmış, teşhis amaçlı dışkı ve kan serumu örnekleri
alınmıştır. Mycobacterium avium subsp. paratuberculosis (MAP) serum örneklerinde ELISA yöntemi kullanılarak tespit
edildi. Süt sığırlannda paratuberküloz’un prevalansı, dışkı örneklerinde; Ziehl-Neelsen boyama ile %4.59, ELISA testi
ile ise %31.80 oranında pozitif tespit edilmiştir

Anahtar Kelimeler

Sezgin ŞENTÜRK Mihriban ÜLGEN Zafer MECİTOĞLU Sertan ÇATIK Gülşah DEMİR A.Ebru BORUM ELISA Paratüberküloz Ziehl Neelsen Boyama 2014 Etlik Veteriner Mikrobiyoloji Dergisi

Kaynakça

  • Abendan N, Sevilla IA, Prieto JM , Garrido JM, Juste RA, Alonso-Hearn M, (2013). Mycobacterium aviumsubspeciesparatuberculosis isolates from sheep and goats Show reduced persistence in bovine macrophages than cattle,bison, deer and wild boar strains regardless of genotype. Vet Microbiol. 163, 325-334.
  • Antognoli MC, Hirst HL, Garry FB, Salman MD, (2007). Immune Response to and Faecal Shedding of Mycobacterium avium ssp. paratuberculosis in Young Dairy Calves, and the Association Between Test Results in the Calves and the Infection Status of their Dams. Zoonoses Public Hlth . 54, 152-159.
  • Collins MT, and Sockett DC, (1993a). Accuracy and economics of USDA-Licensed enzyme-linked immunosorbent assay for bovine paratuberculosis. J Am Vet Med Assoc. 203, 1456-1463.
  • Collins MT, Sockett DC, Ridge F, (1993b). Evaluation of a commercial enzyme-linked immunosorbent assay for Johne’s disease. J Clin Microbiol. 5, 52-55.
  • Collins MT, Gardner IA, Garry FB, Roussel AJ, Wells JS, (2006). Consensus recommendations on diagnostic testing for the detection of paratuberculosis in cattle in the United States. JAVMA-J AM VET MED A 229(12), 1912-1919.
  • Çetinkaya B, Erdoğan HM, Morgan KL, (1997). Risk factors for Bovine Paratuberculosis. II. The multiple analysis of risk ractors for Bovine Paratuberculosis. Turk J Vet Anim Sci. 21, 303-306.
  • Çetinkaya B, Muz A, Ertaş HB, Öngör H, Sezen İY, Gülcü HB, (2000). Süt ineklerinde Paratüberküloz Prevalansının Polimeraz Zincir Reaksiyonu (PZR) ile Saptanması. Turk J Vet Anim Sci. 24, 371-379.
  • De Kantor IN, Weyer KE, (2004). 1998-last update,laboratory services in tuberculosis control, part II.
  • Dieguez FJ, Gonzalez AM, Menendez S, Vilara MJ, Sanjuana ML, Yus E, Arnai I, (2009). Evaluation of four commercial serum ELISAs for detection of Mycobacterium avium subsp. paratuberculosis infection in dairy cows. Vet J. 180(2), 2231-235.
  • Fujiki A, (2001). Direct smear examination. In: TB Bacteriology Examination to Stop TB. The Research Institute of Tuberculosis, ed. Fujiki A, Japan International Cooperation Agency JINNOU Co. 7.
  • Garcia RR, Perez-de-la-Lastra JM, Vicente J, Ruiz- Fons F, Garrido JM, Gortazar C, (2008). Large-scale ELISA testing of Spanish red deer for paratuberculosis. Vet Immunol Immunopathol.124, 75-81.
  • Gasteiner J, Wenzli H, Fuchs K, Jark U, Baumgartner W, (1999). Serological Cross-sectional Study of Paratuberculosis in Cattle in Austria . J Vet Med. B 46, 457-466.
  • Gilardoni LR, Paolıcchi FA, Mundo SL. (2012). Bovine paratuberculosis: a review of the advantages and disadvantages of different diagnostic tests. Rev Argent Microbiol. 44, 201-215.
  • Lambeth C, Reddacliff LA, Windsor P, Abbott KA, MacGregor H, Whittington RJ, (2004). Intrauterin and transmammary transmission of Mycobacterium avium subsp. paratuberculosis in sheep. Aust Vet J. 82, 504-508.
  • Makav M, Gökçe E, (2013). Kars Yöresi Sığırlarında Subklinik Paratuberkülozun Seroprevalansı. Kafkas Univ Vet Fak Derg. 19 (5), 913-916.
  • Mura M, Bull TJ, Evans H, Sidi-Boumedine K, McMinn L, Rhodes G, Pickup R, Hermon-Taylor J, (2006). Replication and long-term persistence of bovine and human strains of Mycobacterium avium subsp. paratuberculosis within Acanthamoeba polyphaga. Applied Environ Microbiol 72, 854-859.
  • Naser SA, Ghobrial G, Romero C, Valentine JF, (2004). Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn’s disease. Lancet 364, 1039-1044.
  • Nielsen SS, Toft N, (2008a). Ante mortem diagnosis of paratuberculosis: A review of accuracies of ELISA, interferon-ɣ assay and faecal culture techniques. Vet Microbiol. 129, 217-235.
  • Nielsen SS, (2008b). Transitions in diagnostic tests used for detection of Mycobacterium avium subsp. paratuberculosis. Vet Microbiol. 132, 274-282.
  • Öztürk D, Pehlivanoğlu F, Tok AA, Günlü S, Güldali Y, Türütoğlu H, (2010). Seroprevalence of paratuberculosis in the Burdur province (Turkey), in dairy cattle using the enzyme linked immunosorbent assay (ELISA). Israel J Vet Med. 65, 53-57.
  • Paolicchi A, Zumarraga MJ, Gioffre A., Zamorano P, Morsella C, Verna1A, Cataldi A, Alito A, Romano M, (2003). Application of Different Methods for the Diagnosis of Paratuberculosis in a Dairy Cattle Herd in Argentina. J Vet Med. B 50, 20-26.
  • Rajukumar K, Tripathi BN, Kurade NP, Parihar NS, (2001). An Enzyme -liınked Immunosorbent Assay using Immuno affinity-purified Antigen in the Diagnosis of Caprine Paratuberculosis and Its Comparision with Conventional ELISA. Vet Res Commun. 25, 539-553.
  • Senturk S, Metcitoglu Z, Ulgen M, Borum E, Temizel E, Kasap S, (2009). Evaluation of serum iron and iron binding capacity in cows with paratuberculosis Tierarztl Prax. 6,375-378.
  • Shahmoradi AH, Arefpajohi Reza, Tadayon K, Mosavari N, (2008). Paratuberculosis in Holstein-Friesian cattle farms in Central Iran Trop Anim Health Prod 40, 169-173.
  • Shin SJ, Cho D, Collins MT, (2008). Diagnosis of bovine paratuberculosis by a novel enzyme-linked imunosorbent assay based on early secreted antigens of Mycobacterium avium subsp. paratuberculosis. Clin Vaccine Immunol, 15, 1277-1281.
  • Singh SV, Singh AV, Singh R, Sharma S, Shuklab N, Misraa S, Singh PK, Sohala JS, Kumar H, Patil PK, Misrad P, Sandhu KS, (2008). Sero-prevalence of Bovine Johne’s disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis ‘Bison type’ genotype of goat origin. Comp Immunol Microb. 31, 419-433.
  • Stabel, JR, (1996). Production of gamma-interferon by peripheral blood mononuclear cells: an important diagnostic tool for detection of subclinical paratuberculosis. J Vet Diagn Invest 8, 345-350.
  • Sweeney RW, Whitlock RH, Rosenberger A, (1992). Mycobacterium avium subsp. paratuberculosis cultured from milk and suprammamary lymph nodes of infected asyntomatic cows. J Clin Microbiol. 30, 166-71.
  • Uzoigwe JC, Khaitsa ML, Gibbs PS, (2007). Epidemiological evidence for Mycobacterium avium subspecies paratuberculosis as a cause of Crohn’s disease. Epidemiol Infect. 135, 1057-1068.
  • Yıldırım D, Civelek T, (2013). Prevalence of subclinical paratuberculosis in dairy cattle in Uşak Region, Kars Yöresi Sığırlarında Subklinik Paratuberkülozun Seroprevalansı Kafkas Univ Vet Fak Derg. 19 (1), 121-126.
  • Yooa HS, Shin SJ, (2012). Recent research on bovine paratuberculosis in South Korea. Vet Immunol Immunopathol.148, 23-28.
Toplam 31 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Cerrahi
Bölüm Araştırma Makaleleri
Yazarlar

A.ebru Borum Bu kişi benim

Sertan Çatık Bu kişi benim

Zafer Mecitoğlu Bu kişi benim

Gülşah Demir Bu kişi benim

Mihriban Ülgen Bu kişi benim

Sezgin Şentürk Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2014
Gönderilme Tarihi 1 Aralık 2014
Yayımlandığı Sayı Yıl 2014 Cilt: 25 Sayı: 1

Kaynak Göster

APA Borum, A., Çatık, S., Mecitoğlu, Z., Demir, G., vd. (2014). ELISA ve fekal bakteriyoskopi ile sığırlarda Paratuberküloz prevalansının belirlenmesi. Etlik Veteriner Mikrobiyoloji Dergisi, 25(1), 1-5.


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