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The Diagnosis of Bacterial Kidney Disease (BKD) From Cultured Rainbow Trout (Oncorhynchus Mykiss)

Year 2008, Volume: 19 Issue: 1, 1 - 8, 01.06.2008

Abstract

In this project, totally 360 samples from 5 rainbow trout farms on Kesikköprü barrage lake and aseptically 120 kidney
tissues about 6-12 months rainbow trout from one rainbow trout farm from 2005 to 2007 were collected for isolation and identification
of R.salmoninarum;. Ovarian (coelemic) fluids were collected from 120 mature females of fish farming on Safranbolu.
KDM-2 medium was used for isolation from samples. R.salmoninarum was not isolated on totally 600 samples. DFAT and nested-
PCR results of all samples were negative.
As a result Bacterial Kindey Disease was not detected in Ankara and Safranbolu. In our laboratories, Bacterial Kindey Disease
could be diagnosed rapidly by conventional isolation and identification with DFAT and nested-PCR on rainbow trout.

References

  • Austin B, Austin DA, eds., (1999). Bacterial Fish Pathogens: Disease in Farmed and Wild Fish, Third Edition. Springer-Praxis, Chishester, UK.
  • Bruno DW, (2004). Prevalance and diagnosis of bacterial kidney disease (BKD) in Scotland between 1990 and 2002. Dis Aquat Organ. 59 (2), 125-30.
  • Bullock GL, Herman RL, (1980). Bacterial Kidney Disease of Salmonid fishes caused by Renibacterium salmoninarum. Fish Disease Leaflet 78, U.S. Fish and Wildlife Service, National Fisheries Research Center- Leetown, National Fish Research Laboratory, Box 700, Kearneysville, West Virginia.
  • Bullock GL, Stuckey HM, (1975). Fluorescent antibody identification and detection of the Corynebacterium causing kidney disease in salmonids. J Fisheries Res Board Can. 32. 2224-2227.
  • Chase DM, Pascho RJ, (1998). Development of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a higly sensitive method for detection of the bacterium in Salmonid kidney. Dis Aquat Org. 34. 223- 229.
  • Fryer JL, Lannan CN, (1993). The history and current status of Renibacterium salmoninarum causitive agent of bacterial kidney disease in Pacific salmon. Fisheries Resarch. 17, 15-33.
  • Halıcı G, İstanbulluoğlu E, Arda M, (1977). Bayındır Barajı alabalık yetiştirme istasyonunda görülen bakteriyel böbrek hastalığı ve sağaltımı. İstanbul Üni Vet Fak Derg 3 (1-2), 22-27.
  • Jonsson E, (2002). Bacterial Kidney Disease in salmonid fish. PhD Thesis, Acta Universitatis agriculturae Sueciae Veterinaria, vol. 116.
  • Magnusson HB, Fridjonsson OH, Andresson OS, Benediktsdottir E, Gudmundsdottir S, Anderstottir V, (1994). Renibacterium salmoninarum, the causitive agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences. Appl Environ Microbiol. 60 (12), 4580-4583.
  • Manual of Diagnostik Tests for Aquatic animals 2006, (OIE) Bacterial Kidney Disease (Renibacterium salmoninarum) Part 2. Section 2.1, Chapter 2.1.11 p:1-19
  • Miriam A,Griffiths SG, Lovely JE, Lynch WH, (1997). PCR and probe-PCR assays to monitor broodstock Atlantic salmon (Salmo salar L.) ovarian fluid and kidney tissue for presence of DNA of the fish pathogen Renibacterium salmoninarum. J Clin Microbiol. 35(6). 1322-1326.
  • Ordal EJ, Earp BJ, (1956). Cultivation and transmission of etiological agent of kidney disease in salmonid fishes. Proceedings of the Society of Experimental Biology and Medicine. 92, 85-88.
  • Pascho JR, Chase D, McKibben CL, (1998). Comparison of the membrane-filtration fluorescent antibody test, the enzyme-linked immunosorbent assay and the polymerase chain reaction to detect Renibacterium salmoninarum in salmonid ovarian fluid. J Vet Diag İnvest. 10, 60-66.
  • Sarıeyyüpoğlu M, Muz A, Özdemir Y, (1985). Ovacık- Tunceli alabalık yetiştirme istasyonunda oluşan bakteriyel böbrek hastalığı. Ege Üni Su Ürün Derg. 2 (5-6),71-78.
  • White MR, Wu CC, Albregts SR, Wu CC, (1995). Comparison of diagnostic tests for bacterial kidney disease in juvenile steelhead trout (Oncorhynchus mykiss). J Vet Diag Invest 74, 494-499.

Kültürü Yapılan Gökkuşağı Alabalıklarında (Oncorhynchus Mykiss) Bakteriyel Böbrek Hastalığı (BKD)’nın teşhisi

Year 2008, Volume: 19 Issue: 1, 1 - 8, 01.06.2008

Abstract

Bu projede Renibacterium salmoninarum izolasyon ve identifikasyonu için, 2005-2007 yılları arasında, Kesikköprü Baraj
Göleti’nde yer alan 5 adet alabalık işletmesinden toplam 360 adet; Safranbolu’da yer alan 1 adet alabalık işletmesinden 120 adet 6-12
aylık alabalıklardan böbrek örnekleri aseptik koşullarda alındı. Safranbolu’da yer alan 1 adet alabalık işletmesinde 120 adet olgun dişi
balıklardan ovaryum sıvısı uygun koşullarda alındı. Toplanan örneklerden izolasyon için KDM-2 medium kullanıldı. Toplam 600
adet örnekten hiç R.salmoninarum izolasyonu olmadı. Aynı örneklerden DFAT ve nested-PCR testleri yapıldı. Örneklerin hiçbirinde
etkene rastlanılamadı.
Sonuç olarak Ankara ve Safranbolu’da Bakteriyel Böbrek Hastalığı saptanamamıştır. Laboratuarımızda alabalıklarda konvansiyonel
izolasyon ve identifikasyon yanında DFAT ve nested-PCR gibi testlerle Bakteriyel Böbrek Hastalığı kısa sürede teşhis edilebilir
hale gelmiştir.

References

  • Austin B, Austin DA, eds., (1999). Bacterial Fish Pathogens: Disease in Farmed and Wild Fish, Third Edition. Springer-Praxis, Chishester, UK.
  • Bruno DW, (2004). Prevalance and diagnosis of bacterial kidney disease (BKD) in Scotland between 1990 and 2002. Dis Aquat Organ. 59 (2), 125-30.
  • Bullock GL, Herman RL, (1980). Bacterial Kidney Disease of Salmonid fishes caused by Renibacterium salmoninarum. Fish Disease Leaflet 78, U.S. Fish and Wildlife Service, National Fisheries Research Center- Leetown, National Fish Research Laboratory, Box 700, Kearneysville, West Virginia.
  • Bullock GL, Stuckey HM, (1975). Fluorescent antibody identification and detection of the Corynebacterium causing kidney disease in salmonids. J Fisheries Res Board Can. 32. 2224-2227.
  • Chase DM, Pascho RJ, (1998). Development of a nested polymerase chain reaction for amplification of a sequence of the p57 gene of Renibacterium salmoninarum that provides a higly sensitive method for detection of the bacterium in Salmonid kidney. Dis Aquat Org. 34. 223- 229.
  • Fryer JL, Lannan CN, (1993). The history and current status of Renibacterium salmoninarum causitive agent of bacterial kidney disease in Pacific salmon. Fisheries Resarch. 17, 15-33.
  • Halıcı G, İstanbulluoğlu E, Arda M, (1977). Bayındır Barajı alabalık yetiştirme istasyonunda görülen bakteriyel böbrek hastalığı ve sağaltımı. İstanbul Üni Vet Fak Derg 3 (1-2), 22-27.
  • Jonsson E, (2002). Bacterial Kidney Disease in salmonid fish. PhD Thesis, Acta Universitatis agriculturae Sueciae Veterinaria, vol. 116.
  • Magnusson HB, Fridjonsson OH, Andresson OS, Benediktsdottir E, Gudmundsdottir S, Anderstottir V, (1994). Renibacterium salmoninarum, the causitive agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences. Appl Environ Microbiol. 60 (12), 4580-4583.
  • Manual of Diagnostik Tests for Aquatic animals 2006, (OIE) Bacterial Kidney Disease (Renibacterium salmoninarum) Part 2. Section 2.1, Chapter 2.1.11 p:1-19
  • Miriam A,Griffiths SG, Lovely JE, Lynch WH, (1997). PCR and probe-PCR assays to monitor broodstock Atlantic salmon (Salmo salar L.) ovarian fluid and kidney tissue for presence of DNA of the fish pathogen Renibacterium salmoninarum. J Clin Microbiol. 35(6). 1322-1326.
  • Ordal EJ, Earp BJ, (1956). Cultivation and transmission of etiological agent of kidney disease in salmonid fishes. Proceedings of the Society of Experimental Biology and Medicine. 92, 85-88.
  • Pascho JR, Chase D, McKibben CL, (1998). Comparison of the membrane-filtration fluorescent antibody test, the enzyme-linked immunosorbent assay and the polymerase chain reaction to detect Renibacterium salmoninarum in salmonid ovarian fluid. J Vet Diag İnvest. 10, 60-66.
  • Sarıeyyüpoğlu M, Muz A, Özdemir Y, (1985). Ovacık- Tunceli alabalık yetiştirme istasyonunda oluşan bakteriyel böbrek hastalığı. Ege Üni Su Ürün Derg. 2 (5-6),71-78.
  • White MR, Wu CC, Albregts SR, Wu CC, (1995). Comparison of diagnostic tests for bacterial kidney disease in juvenile steelhead trout (Oncorhynchus mykiss). J Vet Diag Invest 74, 494-499.
There are 15 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Original Article
Authors

Sibel Özkök This is me

Selahattin Şen This is me

Hikmet Ün This is me

Publication Date June 1, 2008
Submission Date December 1, 2008
Published in Issue Year 2008 Volume: 19 Issue: 1

Cite

APA Özkök, S., Şen, S., & Ün, H. (2008). Kültürü Yapılan Gökkuşağı Alabalıklarında (Oncorhynchus Mykiss) Bakteriyel Böbrek Hastalığı (BKD)’nın teşhisi. Etlik Veteriner Mikrobiyoloji Dergisi, 19(1), 1-8.

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