The Diagnosis of Bacterial Kidney Disease (BKD) From Cultured Rainbow Trout (Oncorhynchus Mykiss)

Year 2008, Volume: 19 Issue: 1, 1 - 8, 01.06.2008

Sibel Özkök Selahattin Şen Hikmet Ün

Abstract

In this project, totally 360 samples from 5 rainbow trout farms on Kesikköprü barrage lake and aseptically 120 kidney
tissues about 6-12 months rainbow trout from one rainbow trout farm from 2005 to 2007 were collected for isolation and identification
of R.salmoninarum;. Ovarian (coelemic) fluids were collected from 120 mature females of fish farming on Safranbolu.
KDM-2 medium was used for isolation from samples. R.salmoninarum was not isolated on totally 600 samples. DFAT and nested-
PCR results of all samples were negative.
As a result Bacterial Kindey Disease was not detected in Ankara and Safranbolu. In our laboratories, Bacterial Kindey Disease
could be diagnosed rapidly by conventional isolation and identification with DFAT and nested-PCR on rainbow trout.

Kültürü Yapılan Gökkuşağı Alabalıklarında (Oncorhynchus Mykiss) Bakteriyel Böbrek Hastalığı (BKD)’nın teşhisi

Abstract

Bu projede Renibacterium salmoninarum izolasyon ve identifikasyonu için, 2005-2007 yılları arasında, Kesikköprü Baraj
Göleti’nde yer alan 5 adet alabalık işletmesinden toplam 360 adet; Safranbolu’da yer alan 1 adet alabalık işletmesinden 120 adet 6-12
aylık alabalıklardan böbrek örnekleri aseptik koşullarda alındı. Safranbolu’da yer alan 1 adet alabalık işletmesinde 120 adet olgun dişi
balıklardan ovaryum sıvısı uygun koşullarda alındı. Toplanan örneklerden izolasyon için KDM-2 medium kullanıldı. Toplam 600
adet örnekten hiç R.salmoninarum izolasyonu olmadı. Aynı örneklerden DFAT ve nested-PCR testleri yapıldı. Örneklerin hiçbirinde
etkene rastlanılamadı.
Sonuç olarak Ankara ve Safranbolu’da Bakteriyel Böbrek Hastalığı saptanamamıştır. Laboratuarımızda alabalıklarda konvansiyonel
izolasyon ve identifikasyon yanında DFAT ve nested-PCR gibi testlerle Bakteriyel Böbrek Hastalığı kısa sürede teşhis edilebilir
hale gelmiştir.

Keywords

Etlik Veterinerlik Mikrobiyoloji Dergisi, Kültür, Gökkuşağı Balıkları, Bakteri, Böbrek, Sibel ÖZKÖK, Selahattin ŞEN, Hikmet ÜN, 2008

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