Türkiye’nin Orta Karadeniz Bölgesindeki Gökkuşağı Alabalıkları (Oncorhynchus mykiss, Walbaum, 1792)’ nda Laktokokkozis ve Lactococcus Garvieae Etkeninin Antimikrobiyal Duyarlılığı

Year 2013, Volume: 24 Issue: 1, 7 - 12, 01.12.2013

Türkay Öztürk Behire Işıl Didinen Gaye Doğan Ahmet Özer Recep Bircan

Abstract

Bu çalışmada, Türkiye’nin Karadeniz Bölgesindeki gökkuşağı alabalıklarından Lactococcus garvieae izole edildi.
Hastalık salgını ortalama 16.7°C su sıcaklığında Nisan-Mayıs 2009 aylarında görüldü. Hasta gökkuşağı alabalıklarında
klinik olarak durgunluk, iştahsızlık, renkte koyulaşma, tek ya da çift taraflı eksoftalmus, korneada opaklaşma,
gözlerde, pektoral ve anal yüzgeç tabanlarında hemoraji, karında şişkinlik, vücut yüzeyinde yaralar görüldü. Nekropside
vücut boşluğunda kanlı-renkli bir sıvının varlığı, barsak ve midede sarı berrak renkte sıvı ile karakterize asites, dalak ve
böbrekte büyüme, renklerinde koyulaşma, karaciğerde büyüme, renginde açılma ve peteşiyel kanamaların olduğu tespit
edildi. Hastalık etkenini izole etmek için yaklaşık 50-100 g ağırlığında hasta 20 adet gökkuşağı alabalığı bakteriyolojik
incelemelerde kullanıldı. Bakterilerin identifikasyonunda konvansiyonel testler kullanıldı ve hastalık etkeni L.garvieae
olarak tanımlandı. İzole edilen L.garvieae suşu, amoxicillin, amoxicillin/clavulanic acid, cephalothin, chloramphenicol,
doxycycline, enroflaxacin, pristinamycine ve tetracycline duyarlı olduğu tespit edildi.

Keywords

Etlik Veteriner Mikrobiyoloji Dergisi, Lactococus Garvieae, Fenotipik İdentifikasyon, Antimikrobiyal Duyarlılık, Gökkuşağı Alabalık, Türkay ÖZTÜRK, Behire Işıl DİDİNEN, Gaye DOĞAN, Ahmet ÖZER, Recep BİRCAN, 2013

Lactococcosis in Rainbow Trout (Oncorhynchus mykiss,Walbaum, 1792) in the Middle Black Sea Region in Turkey and Antimicrobial Susceptibility of the Aetiological Agent, Lactococcus Garvieae

Abstract

In this research study, we isolated Lactococcus garvieae from rainbow trout in the middle Black Sea region
in Turkey. The diseases outbreak occurred at average water temperature of 16.7°C during April-May 2009. Clinically
stagnation, inapetence, darkening of the skin, exophtalmia, opacification in the cornea, hemorrhages in the eyes and at
the base of pectoral and anal fins, swollen abdomen and wounds on the body surface were observed in infected fish. In
necropsy; the existence of accumulation of bloody fluid in the body cavity, ascites in the intestines and the stomach characterized
with a yellowish-colored liquid, enlarged and darkening in the spleen and kidney, fading in colour, existence
of petechial hemorrhages and enlarged in the liver were detected. In order to isolate the aetiological agent, 20 infected
rainbow trout weighting between 50-100 gr were used. As the result of conventional tests used in the identification of the
bacteria, the aetiological agent was identified as L.garvieae. The isolated L.gavieae strain was determined as susceptible
to amoxicillin, amoxicillin/clavulanic acid, cephalothin, chloramphenicol, doxycycline, enroflaxacin, pristinamycine
and tetracycline.

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