Vero hücrelerinde ısıya dayanıklı sığır vebası aşı üretimi

Year 2010, Volume: 21 Issue: 1, 23 - 30, 01.06.2010

Özden Kabaklı Elvin Çalışkan A. Burak Güngör Süreyya Yöndem İlkay Demirhan

Abstract

Vero hücrelerinde üretilen bir sığır vebası aşısının termostabilitesi %5 laktalbumin hidrolizat ve %10 sukrozdan oluşan stabilizatör ve 74 saat süren liyofilizasyon protokolu ile gerçekleştirildi. Stabilizatör ve liyofilizasyon protokolu +37ºC, +42ºC, +45ºC ve +56ºC’deki hızlandırılmış stabilite testi ile test edildi ve bu verilerden Arhenius eğrisi yapıldı. Bu metotla üretilen liyofilize sığır vebası aşısı minimum gerekli infektif titre olan 10-2.5 DKID50 /ml titreyi +37ºC’de 30 günden fazla sürdürmüştür. Aşının aktivitesi sığırlarda antikor titresi ile test edildi. Sulandırılmış aşının stabilitesi üç farklı dilüent ile test edildi. Sonuç olarak aşı virusunun 1 molar magnezyum sülfat solüsyonu ve normal fizyolojik tuzlu su ile sulandırıldığında, +37°C gibi yüksek ısıya sahip bir ortamda 4 saatten daha fazla infektif titresini koruduğu belirlendi.

Keywords

Canlı aşı, ısıya dayanıklı, sığır vebası, stabilite, vero hücre., Özden KABAKLI, Elvin ÇALIŞKAN, A. Burak GÜNGÖR, Süreyya YÖNDEM, İlkay DEMİRHAN, 2010, Etlik Veteriner Mikrobiyoloji Dergisi

Production of a thermostable vero-cell adapted rinderpest vaccine

Abstract

The thermo stability of a rinderpest vaccine produced on vero cells were evaluated using 5% lactalbumin hydrolysate–10% sucrose as a stabilizer and a lyophilization protocol that is takes 74 hours. The stabilizer and lyophilization schedules were examined using accelerated stability testing at +37ºC, +42ºC,+45ºC and +56ºC, and an Arrhenius plot was based on from these data. A lyophilized rinderpest vaccine was maintained the minimum required dose of 10-2.5 TCID50 /ml more than 30 days at +37ºC. The biological activity of the vaccine by measured with antibody titration in cattle. The stability of the reconstituted vaccine was examined using three different diluent preparations. Finally, the infectivity of vaccine was stable even at high temperatures up to 4 h after reconstitution with magnesium sulphate molar solution or normal physiological saline.

Keywords

Live vaccine, rinderpest, stability, thermostability, vero cells.

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