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Diagnosis of Mycoplasma Gallisepticum Infection in Chickens By Polymerase Chain Reaction (PCR)

Year 2011, Volume: 22 Issue: 2, 45 - 48, 29.12.2011

Abstract

Mycoplasma gallisepticum causes important economic losses and it spreads vertically and horizontally in chickens. Although a lot of methods are used for detection of this bacterium, agents can be obtained and sensitive and rapid result can be achieved by PCR based diagnosis method regardless of isolation. In this study, trachea and tracheal swab samples collected from various broiler flocks were evaluated. 14 of 26 investigated flocks (%53,8) were found to be M.gallisepticum positive. 28 of all trachea samples from 14 flocks and tracheal swabs which were taken 5 (%19,2) flocks considered to be positive. This result showed that M.gallisepticum infections can be diagnosed rapidly and more specifically and more positive results can be achieved in tracheal organ samples than tracheal swabs.

References

  • Anonim, (2008). Avian Mycoplasmosis (Mycoplasma gallisepticum, M. Synoviae) in OIE Terrestrial Manual Online. Chapter. 2.3.5. p. 482-496. Erişim adresi: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.03.05_%20AVIAN_MYCO.pdf, Erişim tarihi: 25.04.2011.
  • Anonim, (2007). Avian Mycoplasmosis (Mycoplasma gallisepticum), Pleuropneumonia–like Organism (PPLO) Infection, Chronic Respiratory Disease, Infectious Sinusitis, House Finch Conjunctivitis. Erişim adresi: http://www.cfsph.iastate.edu/Factsheets/pdfs/avian_mycoplasmosis_mycoplasma_gallisepticum.pdf , Erişim tarihi: 20.04.2011.
  • Bağcıgil F, (2002). Tavuklarda mycoplasma gallisepticum infeksiyonunun tanısında bakteriyolojik ve serolojik yöntemlerin polymerase chain reaction (PCR) ile karşılaştırılması. Doktora Tezi. İstanbul Ünv. Sağlık Bilimleri Enst. İstanbul.
  • Behbahan GG, Asasi K, Afsharifar AR, Poubakhsh SA, (2005). Isolation and detection of Mycoplasma gallisepticum by polymerase chain reaction and restriction fragment length polymorphism. Iranian J Vet Res Uni Shiraz. 6, 35-41.
  • Buim MR, Mettifogo E, Timenetsky J, Kleven S, Ferreira AJP, (2009). Epidemiological survey on Mycoplasma gallisepticum and M.synoviae by Multiplex PCR in commercial poultry. Pesq Vet Bras. 29, 552-556.
  • Esendal Ö, (2002). Mikoplazma İnfeksiyonları. Kanatlı Hayvan Hastalıkları. Edt: İzgür M, Akan M. Medisan, Ankara.p.79-85.
  • Evans JD, Thornton DL, Branton SL, (2009). Diagnosis of Mycoplasma gallisepticum from broiler Breeder Flock: Comparison of Three Diagnostic Methods. Int J Poult Sci. 8, 104-107.
  • Feberwee A, Mekkes DR, Wit JJ, Hartman EG, Pijpers A, (2005). Comparison of Culture, PCR and Different Serologic Tests for Detection of Mycoplasma gallisepticum and Mycoplasma synoviae Infections. Avian Dis. 49, 260-268.
  • Gharaibeh S, Roussan D, (2008). The Use of Molecular Techniques in Isolation and Characterization of Mycoplasma gallisepticum from Commercial Chickens in Jordan. Int Poult Sci. 7, 28-35.
  • Kahya S, Temelli S, Eyigör A, Çarlı KT, (2010). Real-Time PCR culture and serology for diagnosis of Mycoplasma gallisepticum in chicken breeder flocks. Vet Microbiol. 144, 319-324.
  • Khan MI, (2003). Multiplex PCR of Avian Pathogenic Mycoplasmas. Methods in Molecular Biology. Edt: Sachse K, Frey J. 216, 223-229.
  • Kleven SH, Jordan FTW, Bradbury JM, (2004). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Avian Mycoplasmosis. Fifth edition. Paris: Office International des Epizooties, Chapter 2.7.3. p.1-24.
  • Levisohn S, (2000). Avian Mycoplasmosis Biotechnology Applied in Peru. Kimron Veterinary Institute, Bet Dagan Israel.
  • Levisohn S, Kleven SH, (2000). Avian Mycoplasmosis (Mycoplasma gallisepticum). Rev Sci Tech Off Epiz. 19, 425-442.
  • Marois C, Dufour-Gesbert F, Kempf I, (2002). Polymerase Chain Reaction for Detection of Mycoplasma gallisepticum in enviromental samples. Avian Pathol. 31, 163-168.
  • Moalic P, (2002). Improving mycoplasmosis control using PCR technology. World Poult. 18, 38-39.
  • Roussan D.A, Haddad R, Khawaldeh G, (2008). Molecular Survey of Avian Respiratory Pathogens in Commercial Broiler Chicken Flocks with Respiratory Disease in Jordan. Poult Sci. 87, 444-448.
  • Zain ZM, Bradbury JM, (1996). Optimising the conditions for isolation of Mycolasma gallisepticum collected on applicator swabs. Vet Microbiol. 49, 45-57.

Tavuklarda Mycoplasma Gallisepticum İnfeksiyonunun Polimeraz Zincir Reaksiyonu (PCR) ile Teşhisi

Year 2011, Volume: 22 Issue: 2, 45 - 48, 29.12.2011

Abstract

Mycoplasma gallisepticum tavuklarda önemli ekonomik kayıplara neden olarak, vertikal ve horizontal yayılım gösterir. Bu etkenin teşhisi için pek çok metod kullanılmasına rağmen PCR temelli teşhis metodları ile izolasyona gerek kalmadan infeksiyon izlenebilir, yüksek duyarlılıkta hızlı sonuç alınabilir. İncelenen 26 kümesin 14 (%53,8)’ü M.gallisepticum yönünden pozitif bulundu. Materyal orijini dikkate alındığında 14 broyler kümesten alınan trachea örneklerinin 28 adetinden pozitif sonuç alınırken bu kümeslere ait tracheal svab örneklerinden sadece 5 (%19,2) kümese ait olanlarda M.gallisepticum spesifik DNA varlığı saptandı. Bu bulgu özellikle örnekleme aşamasında mikoplazma infeksiyonu şüpheli kümeslerden hem trachea hem de svab örneklerinin alınmasının yararlı olduğunu gösterdi. Bu sonuçlar PCR metodunun kullanılması ile M.gallisepticum infeksiyonlarının daha kısa sürede ve daha spesifik olarak teşhis edilebileceğini ve trachea örneklerinin tracheal svab örneklerine göre daha fazla pozitiflik sağladığını göstermektedir.

References

  • Anonim, (2008). Avian Mycoplasmosis (Mycoplasma gallisepticum, M. Synoviae) in OIE Terrestrial Manual Online. Chapter. 2.3.5. p. 482-496. Erişim adresi: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.03.05_%20AVIAN_MYCO.pdf, Erişim tarihi: 25.04.2011.
  • Anonim, (2007). Avian Mycoplasmosis (Mycoplasma gallisepticum), Pleuropneumonia–like Organism (PPLO) Infection, Chronic Respiratory Disease, Infectious Sinusitis, House Finch Conjunctivitis. Erişim adresi: http://www.cfsph.iastate.edu/Factsheets/pdfs/avian_mycoplasmosis_mycoplasma_gallisepticum.pdf , Erişim tarihi: 20.04.2011.
  • Bağcıgil F, (2002). Tavuklarda mycoplasma gallisepticum infeksiyonunun tanısında bakteriyolojik ve serolojik yöntemlerin polymerase chain reaction (PCR) ile karşılaştırılması. Doktora Tezi. İstanbul Ünv. Sağlık Bilimleri Enst. İstanbul.
  • Behbahan GG, Asasi K, Afsharifar AR, Poubakhsh SA, (2005). Isolation and detection of Mycoplasma gallisepticum by polymerase chain reaction and restriction fragment length polymorphism. Iranian J Vet Res Uni Shiraz. 6, 35-41.
  • Buim MR, Mettifogo E, Timenetsky J, Kleven S, Ferreira AJP, (2009). Epidemiological survey on Mycoplasma gallisepticum and M.synoviae by Multiplex PCR in commercial poultry. Pesq Vet Bras. 29, 552-556.
  • Esendal Ö, (2002). Mikoplazma İnfeksiyonları. Kanatlı Hayvan Hastalıkları. Edt: İzgür M, Akan M. Medisan, Ankara.p.79-85.
  • Evans JD, Thornton DL, Branton SL, (2009). Diagnosis of Mycoplasma gallisepticum from broiler Breeder Flock: Comparison of Three Diagnostic Methods. Int J Poult Sci. 8, 104-107.
  • Feberwee A, Mekkes DR, Wit JJ, Hartman EG, Pijpers A, (2005). Comparison of Culture, PCR and Different Serologic Tests for Detection of Mycoplasma gallisepticum and Mycoplasma synoviae Infections. Avian Dis. 49, 260-268.
  • Gharaibeh S, Roussan D, (2008). The Use of Molecular Techniques in Isolation and Characterization of Mycoplasma gallisepticum from Commercial Chickens in Jordan. Int Poult Sci. 7, 28-35.
  • Kahya S, Temelli S, Eyigör A, Çarlı KT, (2010). Real-Time PCR culture and serology for diagnosis of Mycoplasma gallisepticum in chicken breeder flocks. Vet Microbiol. 144, 319-324.
  • Khan MI, (2003). Multiplex PCR of Avian Pathogenic Mycoplasmas. Methods in Molecular Biology. Edt: Sachse K, Frey J. 216, 223-229.
  • Kleven SH, Jordan FTW, Bradbury JM, (2004). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Avian Mycoplasmosis. Fifth edition. Paris: Office International des Epizooties, Chapter 2.7.3. p.1-24.
  • Levisohn S, (2000). Avian Mycoplasmosis Biotechnology Applied in Peru. Kimron Veterinary Institute, Bet Dagan Israel.
  • Levisohn S, Kleven SH, (2000). Avian Mycoplasmosis (Mycoplasma gallisepticum). Rev Sci Tech Off Epiz. 19, 425-442.
  • Marois C, Dufour-Gesbert F, Kempf I, (2002). Polymerase Chain Reaction for Detection of Mycoplasma gallisepticum in enviromental samples. Avian Pathol. 31, 163-168.
  • Moalic P, (2002). Improving mycoplasmosis control using PCR technology. World Poult. 18, 38-39.
  • Roussan D.A, Haddad R, Khawaldeh G, (2008). Molecular Survey of Avian Respiratory Pathogens in Commercial Broiler Chicken Flocks with Respiratory Disease in Jordan. Poult Sci. 87, 444-448.
  • Zain ZM, Bradbury JM, (1996). Optimising the conditions for isolation of Mycolasma gallisepticum collected on applicator swabs. Vet Microbiol. 49, 45-57.
There are 18 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Original Article
Authors

Seyda Cengiz This is me

Orkun Babacan This is me

Gökçen Dinç This is me

Mehmet Akan This is me

Publication Date December 29, 2011
Submission Date December 1, 2011
Published in Issue Year 2011 Volume: 22 Issue: 2

Cite

APA Cengiz, S., Babacan, O., Dinç, G., Akan, M. (2011). Tavuklarda Mycoplasma Gallisepticum İnfeksiyonunun Polimeraz Zincir Reaksiyonu (PCR) ile Teşhisi. Etlik Veteriner Mikrobiyoloji Dergisi, 22(2), 45-48.

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