Sığır Tüberküloz ve Paratüberküloz Enfeksiyonlarının Antemortem Tanı Yöntemleriyle Ayrımı

Year 2024, Volume: 35 Issue: 1, 8 - 18, 04.07.2024

Halil Pir , Hakan Yardımcı

https://doi.org/10.35864/evmd.1413210

Abstract

Bu araştırmada, Tüberküloz şüpheli işletmelerde bulunan hayvanlara, Sığır Tüberkülozunun antemortem tanı ve ayrımında kullanılan tüberkülin deri testleri (PPD bovine ve avian) sonuçlarından yola çıkılarak, Paratüberküloz enfeksiyonu teşhisi koymak için serolojik olarak incelendi ve fekal bakteriyoskopi yapıldı. Ayrıca Türkiye’de endemik olarak görülen Sığır Tüberküloz hastalığının antemortem tanı yöntemleri karşılaştırılıp, interferon gama (IFN-γ) testinin sensitivite ve spesifite değerleri belirlenerek Sığır Tüberküloz hastalığının eradikasyon çalışmalarına katkı sağlayacak verilerin elde edilmesi amaçlandı. Bu kapsamda Çankırı, Çorum, Ankara, Eskişehir ve Konya bölgelerinden birer adet olmak üzere toplam 5 süt sığırcılığı işletmesinde Tüberküloz şüphesi olan 423 sığıra intradermal tüberkülin testi uygulandı ve bu test altın standart metot olarak belirlenerek, IFN-γ testinin sensitivitesi % 86, spesifitesi % 97 olarak belirlendi. Paratüberküloz enfeksiyonunun tanısı için bu hayvanlara antikor ELISA , fekal bakteriyoskopi ve IFN-γ ELISA yapıldı ve bu testlerin prevalansları sırasıyla % 10,4, % 5,44 ve % 4,96 bulundu ve sığırlardan 4 (%0,95)’ü Map enfeksiyonu tanı yöntemlerinin her birine pozitif sonuç verdi. Sonuç olarak, intradermal tüberkülin testi sonuçlarına benzer sonuçlar veren IFN-γ testinin Sığır Tüberkülozu'nun antemortem tanısında kullanılabileceği sonucuna varıldı. Ayrıca Tüberküloz enfeksiyonunun tanısı için yapılan karşılaştırmalı intradermal tüberkülin testinde PPD aviana pozitif reaksiyon veren hayvanların Paratüberküloz tanısı için kullanılan diğer testlerle desteklenerek nonspesifik reaksiyonların ortaya çıkarılmasında ya da PTB enfekte hayvanların tespit edilmesinde belirleyici rol oynadığı görüldü.

Keywords

ELISA, IFN-γ test, Mycobacterium bovis, Paratüberküloz, Sığır

Ethical Statement

Bu çalışma Ankara Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu tarafından onaylanmıştır (2021-13-106).

Supporting Institution

Bu araştırma Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından TDK-2022-2478 numaralı proje ile desteklenmiştir.

Project Number

TDK-2022-2478

Thanks

Bu çalışma ilk yazarın doktora tezinden türetilmiştir

Differentiation of Bovine Tuberculosis and Paratuberculosis Infections with Antemortem Diagnostic Methods

Abstract

In this study, based on the results of tuberculin skin tests (Bovine and Avian PPD) used in the antemortem diagnosis and differentiation of Bovine Tuberculosis, the animals in the farms with suspected Tuberculosis were serologically examined to diagnose Paratuberculosis infection and fecal bacterioscopy was performed. In addition, it was aimed to obtain data that will contribute to the eradication studies of Bovine Tuberculosis disease by comparing the antemortem diagnostic methods of Bovine Tuberculosis disease, which is endemic in Türkiye and by determining the sensitivity and specificity values of the interferon gamma (IFN-γ) test. In this context, intradermal tuberculin test was applied to 423 cattle with suspected Tuberculosis in a total of 5 dairy cattle farms, one each from Çankırı, Çorum, Ankara, Eskişehir and Konya regions, and this test was determined as the gold standard method and the sensitivity and specificity of the IFN-γ test were determined as 86% and 97%, respectively. For the diagnosis of Paratuberculosis infection, antibody ELISA, fecal bacterioscopy and IFN-γ ELISA were performed on these animals and the prevalence of these tests were 10.4%, 5.44% and 4.96% respectively and 4 (0.95%) of the cattle were positive for each of the diagnostic methods for Map infection. As a result, it was concluded that IFN-γ test, which gives similar results to intradermal tuberculin test results, can also be used in the antemortem diagnosis of Bovine Tuberculosis. Also, in the comparative intradermal tuberculin test for the diagnosis of Tuberculosis infection, avian PPD positive animals were found to play a decisive role in the detection of nonspecific reactions or Paratuberculosis infected animals, supported by other tests used for the diagnosis of Paratuberculosis.

Keywords

Cattle, ELISA, IFN-γ test, Mycobacterium bovis, Paratuberculosis

Ethical Statement

This study was approved by the Ankara University Animal Experiments Local Ethics Committee (2021-13-106).

Supporting Institution

This research was supported by Ankara University, Scientific Research Projects Coordination Office with the project number TDK-2022-2478.

Project Number

TDK-2022-2478

Thanks

This study was derived from the PhD thesis of the first author

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