The Effect of Various Carbon Nanotubes on Barley Germination

Year 2019, Volume: 9 Issue: 2, 53 - 59, 01.08.2019

Hülya Akdemir Merve Seven Şaban Kalay Mustafa Çulha Andrew J. Harvey

Abstract

DOI: 10.26650/experimed.2019.19016

Objective: Carbon nanotubes (CNT), one of
the most important carbon-based nanomaterials, have started to be used in the
fields of biology and medicine. Drug delivery, cancer treatment, biosensors,
biomedical imaging and their use as composite materials in tissue engineering
are some of the biomedical applications. However, their extensive applications
may increase the risk of their being released into the environment. Thus, in
this study, we investigated the effects of different carbon on barley (Hordeum
vulgare L. ‘Zeynelağa’) germination.

Material and Method: In addition to usage
of commercial single-walled carbon nanotubes (SWCNT) and multi-walled carbon
nanotubes (MWCNT), carboxylated multi-walled carbon nanotubes (CCNT) have also
synthesized carboxylated multi-walled carbon nanotubes and characterized them
with FTIR spectroscopy. Later, we investigated the effect of different
concentrations (50 and 100 mg/L) of carbon nanotubes on the germination of
barley seeds.

Results: Compared to control groups,
treatment of barley seeds with different concentrations of three carbon
nanotubes had no effect on barley germination.

Conclusion: It was observed that tested
carbon nanotubes have no toxic effect on barley germination. In addition to
these data, it is also important to investigate whether there is a difference
in the gene expression level in order to understand the actual effect of these
materials.

Cite this article as: Akdemir H, Seven M,
Kalay Ş, Çulha M, Harvey AJ. The Effect of Various Carbon Nanotubes on Barley
Germination. Experimed 2019; 9(2): 53-9.

Keywords

Single- and multi-walled carbon nanotubes, carboxylated carbon nanotubes, barley, biomedical applications

Farklı Karbon Nanotüplerin Arpa Çimlenmesi Üzerindeki Etkileri

Abstract

DOI: 10.26650/experimed.2019.19016

Amaç: Karbon tabanlı nanomalzemelerin en
önemlilerinden biri olan karbon nanotüpler (CNT) özellikle biyoloji ve tıp
alanında kullanılmaya başlanmıştır. İlaç salımı, kanser tedavisi, biyosensör,
biyomedikal görüntüleme ve doku mühendisliğinde kompozit materyaller olarak
kullanımları biyomedikal uygulamalarından bazılarıdır. Ancak, yaygın olarak
kullanılmaları, bu yapıların çevreye salınması riskini doğurmuştur. Bu nedenle
bu çalışmada, farklı özellikteki karbon nanotüplerin arpanın (Hordeum vulgare
L. ‘Zeynelağa’) çimlenmesi üzerindeki etkileri araştırılmıştır.

Gereç ve Yöntem: Ticari tek tabakalı karbon
nanotüp (SWCNT), çok tabakalı karbon nanotüplere (MWCNT) ek olarak, laboratuvar
koşullarında karboksillenmiş çok tabakalı karbon nanotüpler (CCNT) sentezlenmiş
ve FTIR spektrokopisi ile karakterizasyonu gerçekleştirilmiştir. Daha sonra,
farklı konsantrasyonlardaki (50 ve 100 mg/L) karbon nanotüplerin arpa
tohumlarının çimlenmesi üzerindeki etkisi araştırılmıştır.

Bulgular: Kontrol grubuyla
karşılaştırıldığında, 3 karbon nanotüpün kullanılan konsantrasyonlarda arpa
bitkisinin çimlenmesi üzerinde bir etki yaratmamıştır.

Sonuç: Denenen farklı karbon nanotüplerin
arpa çimlenmesi üzerinde doğrudan bir toksik etki yaratmadığı belirlenmiştir.
Elde edilen bu verilere ek olarak, gen ekspresyonu düzeyinde bir farklılık olup
olmadığının araştırılması da bu materyallerin gerçek etkisinin anlaşılması
açısından önemlidir. 

Cite this article as: Akdemir H, Seven M,
Kalay Ş, Çulha M, Harvey AJ. The Effect of Various Carbon Nanotubes on Barley
Germination. Experimed 2019; 9(2): 53-9.

Keywords

Tek ve çok tabakalı karbon nanotüpler, karboksillenmiş karbon nanotüpler, arpa, biyomedikal uygulama

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