Sentetik Tohum

Year 2021, , 68 - 74, 30.08.2021

Meltem Erdem , Hüseyin Uysal

https://doi.org/10.51753/flsrt.943981

Cited By: 2

Abstract

Sentetik tohum, besin maddesi ve koruyucu bileşikler içeren özel maddelerle kaplanmış somatik embriyolardır. Sentetik tohum üretimi, canlı tohum üretemeyen bitkiler için elverişlidir. Sentetik tohumdan bitki elde edilmesi, ilk kez doku kültüründen elde edilen somatik embriyodan suyun uzaklaştırılması yoluyla yapılmıştır. Somatik embriyo kaplanmasında sodyum alginat veya alginik asit matriksleri çok yaygın olarak kullanılmaktadır. Bitki çoğaltımının kolay olması, uzun süre saklanabilmesi, nesli tükenmekte olan bitkilerin neslinin devam ettirilmesi ve üretimlerinin düşük maliyetle yapılabilmesi gibi birçok avantaj sentetik tohumun bitkilerin gelişimi için önemli ölçüde kullanılabilirliğini göstermektedir. Sentetik tohum teknolojisi tohumla çoğaltım imkanı bulunmayan bitkilerin üretiminde de kullanılabilecek çok önemli bir tekniktir. Bu derleme makale, sentetik tohum konusundaki ortaya koyulmuş bilgileri ve yapılmış çalışmaları bir araya getirmek amacıyla yazılmıştır.

Keywords

bitki rejenerasyonu, in vitro kültür, sentetik tohum, Somatik embriyo

Supporting Institution

Aydın Adnan Menderes Üniversitesi, Bilimsel Araştırma Projeleri Birimi

Project Number

ZRF20037

Thanks

Tez çalışması kapsamında gerekli ekipman temininin sağlanabilmesi adına Aydın Adnan Menderes Üniversitesi, Bilimsel Araştırma Projeleri Birimi' ne desteklerinden dolayı teşekkür ederiz.

Synthetic Seed

Abstract

Synthetic seeds are somatic embryos coated with special substances containing preservative compounds. Synthetic seed production has been useful for plants that cannot produce viable seeds. Plant formation from synthetic seed was first made by removing water from the somatic embryo obtained from tissue culture. Sodium alginate or alginic acid matrices are commonly used for somatic embryo coating. Many advantages such as easy plant propagation, long-term storage, perpetuation of endangered plants and low cost production show the significant usability of synthetic seed for the development of plants. Synthetic seed technology is a very important technique that can be used in the production of plants that cannot be reproduced by seed. This review article was written to bring together the information and studies on synthetic seed.

Keywords

in vitro culture, plant regeneration, somatic embryo, synthetic seed

Project Number

ZRF20037

References

• Ara, H., Jaiswal, U., & Jaiswal, V. S. (2000). Synthetic seed: Prospects and limitations. Current Science, 78(12), 1438-1444.
• Arvas, Y. E., & Kocacaliskan, I. (2020). Genetiği değiştirilmiş bitkilerin biyogüvenlik riskleri. Türk Doğa ve Fen Dergisi, 9(2), 201-210.
• Babaoglu, M., Gurel, E., & Ozcan, S. (2001). Bitki Biyoteknolojisi I. Doku Kültürü ve Uygulamaları. (pp. 1-374). Konya Selçuk Üniversitesi Basımevi.
• Baltaci, A., & Arslan, M. (2019). Oğulotu (Melissa officinalis) bitkisinde meristem ve somatik embriyo kültürlerinden sentetik tohum elde etme olanakları. Erciyes Tarım ve Hayvan Bilimleri Dergisi, 2(1), 1-10.
• Bornman, C. H. (1993). Micropropagation and somatic embryogenesis. In: Hayward M. D., Bosemark N. O., Romagosa I., Cerezo M. (eds) Plant Breeding: Principles and Prospects (pp. 246-260). Springer, London.
• Calheiros, M. B. P., Vieira, L. G. E., & Fuentes, S. R. L. (1994). Effects of exogenous polyamines on direct somatic embryogenesis in coffee. Revista Brasileira de Fisiologia Vegetal, 6(2), 109-114.
• Cardoza V. (2008). Tissue culture: The manipulation of plant development. In: Stewart C.N. (eds) Plant Biotechnology and Genetics: Principles, Techniques and Aplications (pp. 113-134). John Wiley & Sons, USA.
• Chawla, H. S. (2002). Introduction to Plant Biotechnology. (pp. 1-538). Science Publishers.
• Colgecen, H., & Toker, M. C. (2006). Sentetik tohum. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi, 7(2), 323-336.
• Daud, N., Taha, R. M., & Hasbullah, N. A. (2008). Artificial seed production from encapsulated micro shoots of Saintpaulia ionantha Wendl. (African violet). Journal of Applied Sciences, 8(24), 4662-4667.
• De Jong, A. J., Schmidt, E. D. L., & de Vries, S. C. (1993). Early events in higher- plant embryogenesis. Plant Molecular Biology, 22, 367-377.
• Fang J., Wetten A., & Hadley P. (2004). Cryopreservation of cocoa (Theobroma cacao L.) somatic embryos for long-term germplasm storage. Plant Science, 166(3), 669-675.
• Finer, J. J. (1995). Direct somatic embryogenesis. In: Gamborg O. L., Philips G. C. (eds) Plant Cell, Tissue and Organ Culture (pp. 91-102). Springer, Berlin, Heidelberg.
• Fujii, J. A., Slade, D., & Redenbaugh, K. (1989). Maturation and greenhouse planting of alfalfa artificial seeds. In Vitro Cellular and Development Biology, 25, 1179-1182.
• Fujii, J. A. A., Slade, D., Aguirre-Rascon, J., & Redenbaugh, K. (1992). Field planting of alfalfa artificial seeds. In Vitro Cellular and Developmental Biology, 28, 73-80.
• Ghosh, B., & Haque, S. M. (2019). Synthetic seeds: An alternative approach for clonal propagation to avoiding the heterozygosity problem of natural botanical seeds. In: Faisal M., Alatar A. A. (eds) Synthetic Seeds (pp. 77-112). Springer, Switzerland.
• Gonulsen, N. (1987). Bitki doku kültürleri, yöntemleri ve uygulama alanları. (pp. 1-78). T.C. Tarım ve Köy İşleri Bakanlığı, Menemen-İzmir.
• Gray, D. J., & Purohit, A. (1991). Somatic embrtogenesis and development of synthetic seed technology. Critical Reviews in Plant Sciences, 10, 33-61.
• Guerra, M. P., Vesco, L. L. D., Ducroquet, J. P. H. J., Nodari, R. O., & Reis, M. S. D. (2001). Somatic embryogenesis in Goiaberia serrana: Genotype response, auxinic shock and synthetic seeds. Revista Brasileira de Fisiologia Vegetal, 13(2), 117-128.
• Hussein, N. B. M., Huyop, F., & Kaya, Y. (2020). An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv. TAPM 26. International Journal of Science Letters, 2(2), 62-71.
• Ibrahim, M. S. D., Hartatti, R. S., Rubiyo, Purwito, A., & Sudarsono. (2013). Direct and indirect somatic embryogenesis on Arabica coffee (Coffea arabica). Indonesian Journal of Agricultural Science, 14(2), 79-86.
• Ipekci, K. Z. (2002). Paulownia elongata’nın doku kültürü sistemlerinin optimizasyonu ve sentetik tohum üretimi, Doktora Tezi, (pp. 1-98) İstanbul Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye.
• Ipekci, Z., & Gozukirmizi, N. (2003). Direct somatic embryogenesis and synthetic seed production from Paulownia elongata. Plant Cell Reports, 22, 16-24.
• Jalali, N., Naderi, R., Shahi-Gharahlar, A., & Teixeira da Silva, J. A. (2012). Tissue culture of Cyclamen spp. Scientia Horticulturae, 137, 11-19.
• Jang, B. K., Cho, J. S., & Lee, C. H. (2020). Synthetic seed technology development and production studies for storage, transport, and industrialization of bracken spores. Plants, 9(9), 1079.
• Khor, E., & Loh, C. S. (2005). Artificial seeds. In: Nedovic V., Willaert R. (eds) Aplications of Cell Immobilization Biotechnology (pp. 527-537). Springer Netherlands.
• Kiran, R. (2013). Amsonia orientalis Decne. (Apocynaceae)’de somatik embriyogenezis ve sentetik tohum üretimi, Yüksek Lisans Tezi, (pp. 1-85). Kocaeli Üniversitesi, Fen Bilimleri Enstiüsü, Türkiye.
• Kitto, S. K., & Janic, J. (1982). Polyox as an artificial seed coat for a sexual embryos. Horticultural Science, 17, 488.
• Kocak, M., Izgu, T., Sevindik, B., Tutuncu, M., Curuk, P., Simsek, O., Kacar, Y. A., Teixeira da Silva, J. A., & Yalcin Mendi, Y. (2014). Somatic embryogenesis of Turkish Cyclamen persicum Mill.. Scientia Horticulturae, 172, 26-33.
• Kumar, G. K. & Thomas, T. D. (2012). High frequency somatic embryogenesis and synthetic seed production in Clitoria ternatea Linn.. Plant Cell, Tissue and Organ Culture, 110, 141-151.
• Kurt, O. (2015). Bitki ıslahı. (pp. 1-326). Samsun Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Yayınları.
• Magray, M. M., Wani, K. P., Chatto, M. A. & Ummyiah, H. M. (2017). Synthetic seed technology. International Journal of Current Microbiology and Applied Sciences, 6(11), 662-674.
• Mikula, A., & Rybczyfiski, J. J. (2001). Somatic embryogenesis of Gentiana genes L. The effect of preculture treatment and primary explant origin on somatic embryogenesis of Gentiana cruciata L., G. pannonica (scop) and G. tibetica (King). Acta Phisiologiae Plantarum, 23(1), 15-25.
• Mikula, A., Rybczynfiski, J. J., Skierski, J., Latkowska, M. J., & Fiuk, A. (2005). Somatic embryogenesis of Gentiana genus IV.: Characterisation of Gentiana cruciata and Gentiana tibetica embryogenic cell suspensions. In: Hvoslef-Eide A. K, Preil W. (eds) Liquid Culture Systems for In Vitro Plant Propagation (pp. 345-358). Springer Netherlands.
• Mohammed, K. T., Javed, M. A., Huyop, F., & Kaya, Y. (2021). Plant tissue culture of Nicotiana tabacum cv. TAPM 26 and its minimum inhibition against herbicide-Dalapon. MANAS Journal of Engineering, 9(1), 35-42.
• Monnier, M. (1990). Induction of embriyogenezis in callus culture. In: Pollard J. W., Walker J. M. (eds) Plant Cell and Tissue Culture (pp. 141-148). Humana Press, New Jersey.
• Murashige, T., & Skoog, F. (1962). A revised medium for rapidgrowth and bioassays with tobacco tissue culture. Phisiologia Plantarum, 15(3), 473-497.
• Murashige, T. (1978). The impact of plant tissue culture on agriculture. In: Thorpe, T. A. (ed) Frontiers of Plant Tissue Culture (pp. 15-26). University of Calgary, Alberta, Canada.
• Nakamura, T., Taniguchi, T., & Maeda, E. (1992). Studies on somatic embryogenesis of coffee by scanning electron microscope. Japanese Journal of Crop Science, 61(3), 476-486.
• Onishi, N., Sakamoto, Y., & Hirrosawa, T. (1994). Synthrtic seeds as and application of mass production of somatic embriyos. Plant Cell, Tissue and Organ Culture, 39, 137-145.
• Oktem, H. A., & Yucel, M. (2012). Bitki gelişiminin yönlendirilmesi. In: Steward N. C., J. R. (ed) Doku Kültürü (pp. 113-134). Nobel Basımevi, Ankara.
• Ozcan, S., Babaoglu, M., & Sancak, C. (2001). Somatik embriyogenezis. In: Babaoglu M., Gürel E., Özcan S. (eds) Bitki Biyoteknolojisi (pp.71-88). Selçuk Üniversitesi Basımevi, Konya.
• Ozyigit, I. I., & Yucebilgili Kurtoglu, K. Y. (2020). Particle bombardment technology and its applications in plants. Molecular Biology Reports, 1-17.
• Parrot, W. A., Merkle, S. A., & Williams, E. G. (1991). Somatic embryogenesis: Potential for usein propagation and gene transfer systems. In: Muray D. R. (ed) Advanced Methods in Plant Breeding and Bitechnology (pp. 158-200). Ist ed, CAB International Press UK.
• Raven, P. H., Evert, R. F., & Eichhorn, S. E. (1999). Biology of plants. (pp. 555-569). WH Freeman and Company/Worth Publishers.
• Ravi, D., & Anand, P. (2012). Production and applications of artificial seeds: A review. International Research Journal of Biological Sciences, 1(5), 74-78.
• Redenbaugh, K., Nichol, J. W., Kossler, M. E., & Paasch, B. D. (1984). Encapsulation of somatic embryos and for artificial seed production. In Vitro Cellular & Developmental Biology - Plant, 20, 256-257.
• Redenbaugh, K., Paasch, B. D., Nichol J. W., Kossler, M. E., Viss, P. R., & Walker, K. A. (1986). Somatic seeds: Encapsulation of asexual plant embryos. Bio / Technology, 4(9), 797-801.
• Redenbaugh, K., Slade, D., Viss, P. R., & Fujii, J. A. A. (1987). Encapsulation of somatic embryos in synthetic seed coats. Horticultural Science, 22(5), 803-809.
• Redenbaugh, K. (1990). Application of artificial seed to tropical crops. Horticultural Science, 25(3), 251-255.
• Rihan, H. Z., Kareem, F., El-Mahrouk, M. E., & Fuller, M. P. (2017). Artificial seeds (principle, aspects and applications). Agronomy, 7(4), 71.
• Sahoo, S. L., Rout, J. R., & Kanungo, S. (2012). Synthetic seeds. In: Sharma H. P., Dogra J. V. V., Misra A. N. (eds) Plant Tissue Culture: Totipotency to Transgenic (pp. 101-114). Agrobios, India.
• Saiprasad, G. V. S. (2001). Artificial seeds and their applications. Resonance, 6, 39-47.
• Sakamoto, Y., Mashiko, T., Suzuki, A., Kawata, H., & Iwasaki, A. (1992). Development of encapsulating technology for synthetic seeds. ISHS Acta Horticulturae 319: International Symposium on Transplant Production Systems, Yokohama, Japan. 71-76.
• Saxena A., Shukla M., & Saxena P. (2019). Synthetic seeds: relevance to endangered germplasm conservation ın vitro. In: Faisal M., Alatar A. (eds) Synthetic Seeds (pp. 21-60). Springer, Cham.
• Schwenkel, H. G., & Winkelmann, T. (1998). Plant regeneration via somatic embryogenesis from ovules of Cyclamen persicum Mill.. Plant Tissue Culture and Biotechnology, 4, 28-34.
• Singh, A. K., Varshney, R., Sharma, M., Agarwal, S. S., & Bansal, K. C. (2006). Regeneration of plants from alginate-encapsulated shoot tips of Withania somnifera (L.) Dunal, a medicinally important plant species. Journal of Plant Physiology, 163, 220-223.
• Singh, D., Pal, S., & Sinha, A. (2020). Artificial seed/synthetic seed production–brief procedure–advantages and limitations. Biotica Research Today, 2(6), 422-424.
• Sokmen, A., & Gurel, E. (2001). Sekonder metabolit üretimi. In: Babaoglu M., Gurel E., Ozcan S. (eds) Bitki Biyoteknolojisi I., Doku Kültürü ve Uygulamaları (pp. 211-261). Selçuk Üniversitesi Basımevi, Konya.
• Soneji, J. R., Rao, P. S., & Mhatre, M. (2002). Germination of synthetic seeds of pineapple (Ananas comosus L. Merr.). Plant Cell Reports, 20, 891-894.
• Sule, R. (2005). Farklı oksin çeşitlerinin Yonca (Medicago sativa L.)’da somatik embriyo oluşumuna etkisi üzerine bir araştırma, Yüksek Lisans Tezi, (pp. 1-70). Mustafa Kemal Üniversitesi, Fen Bilimleri Enstitüsü, Antakya, Türkiye.
• Swamy, M. K., Balasubramanya, S., & Anuradha, M. (2009). Germplasm conservation of patchouli (Pogostemon cablin Benth.) by encapsulation of in vitro derived nodal segments. International Journal of Biodiversity and Conservation, 1(8), 224-230.
• Takamura, T., Miyajima, I., & Matsuo, E. (1995). Somatic embryogenesis of Cyclamen persicum Mill. “Anneke” from aseptic seedlings. Plant Cell Reports, 15, 22-25.
• Tekin, H. I. (2015). Bitki doku kültürü. T.C. Tarım ve Köy İşleri Bakanlığı Alata Bahçe Kültürleri Araştırma Enstitüsü Müdürlüğü, Erdemli-Mersin, https://arastirma.tarimorman.gov.tr/alata/Belgeler/Diger-belgeler/DokuK%C3%BClt%C3%BCr%C3%BCH.%C4%B0.Tekin.pdf, Son erişim 18 Temmuz 2021.
• Tisserat, B. (1985). Embryogenesis, organogenesis, and plant regeneration. In: Dixon R. A. (ed) Plant Cell Culture: A Practical Approach (pp. 79-106). Information Retrieval Limited Press. Oxford, England.
• Tsvetkov, I., Jouve, L., & Hausman, J. F. (2006). Effect of alginate matrix composition on regrowth of in vitro-derived encapsulated apical microcuttings of hybrid apsen. Biologia Plantarum, 50 (4), 722-724.
• Unal, M. (2013). Bitki Angiosperm Embriyolojisi. (pp. 1-280). Nobel Akademik Yayıncılık.
• Uysal, H., Seyis, F., & Kurt, O. (2007). Tarla bitkilerinde melezleme bariyerlerinin aşılmasında alternatif bir yöntem: embriyo kültürü. OMÜ Ziraat Fakültesi Dergisi, 22(1), 116-122.
• Uysal, H. (2021). In vitro propagation of black cumin (Nigella sativa L.) plants. Genetika, 53(1), 295-303.