Recombinant Bacterial Bioremediation of Synthetic Dye Pollution as a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation

Şeyma Akkurt

Recombinant Bacterial Bioremediation of Synthetic Dye Pollution as a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation

Abstract

Synthetic dye discharges from industrial activities pose an increasing threat to aquatic habitats and biodiversity linked to forest ecosystems. Methylene blue (MB), a commonly used cationic dye, exhibits persistent properties in aquatic environments, negatively impacting microbial communities, primary producers, and aquatic fauna, thus disrupting ecosystem balance. Therefore, developing nature-based and environmentally friendly remediation approaches is crucial for protecting ecosystem integrity and ensuring biodiversity sustainability. In this study, the MB decolorization potential of recombinant bacterial cells expressing heterologous bacterial multicopper oxidase enzymes was investigated and compared with wild-type strains. Batch decolorization experiments were performed at different initial MB concentrations and contact times of 2–12 hours. The recombinant strain showed significantly higher performance than the wild-type strain, achieving approximately 51% decolorization within 12 hours. Increasing the contact time increased the decolorization efficiency in both strains, but it was determined that recombinant cells achieved higher decolorization in a shorter time. The findings suggest that recombinant bacterial systems can be environmentally friendly and economical biotechnological tools for controlling synthetic dye pollution. These approaches can contribute to the conservation of aquatic habitats linked to forest ecosystems, thus supporting the sustainability of biodiversity in the long term.

Keywords

References

Abbas, A., Mushtaq, A., Cheema, A.I., et al. (2020). Heterologous expression of azoreductase-encoding gene azrS of Bacillus sp. MR-1/2 for enhanced azo dye decolorization and wastewater treatment. Arch. Microbiol., 202(8):2135–2145, https://doi.org/10.1007/s00203-020-01940-w
Afreen, S., Shamsi, T.N., Baig, M.A., Ahmad, N., Fatima, S., Qureshi, M.I., and Fatma, T. (2017). A novel multicopper oxidase (laccase) from cyanobacteria: purification, characterization with potential in the decolorization of anthraquinonic dye. PLoS One 12(4):e0175144, https://doi.org/10.1371/journal.pone.0175144
Ahuja, S.K., Ferreira, G.M., and Moreira, A.R. (2004). Utilization of enzymes for environmental applications. Crit. Rev. Biotechnol. 24:125–154.
Almaz, Z., and Agircelik, F.N. (2023). Enzymatic degradation of azo dyes methylene blue and congo red with peroxidase purified from cauliflower using affinity chromatography technique: Kinetic study, optimization and metal binding activity. J. Biosci. Bioeng. 136(2):102–108, https://doi.org/10.1016/j.jbiosc.2023.05.011
Ariffin, F., Anuar, C., and Mohamed, N.E. (2022). Biodegradation of methylene blue by bacteria strains isolated from contaminated soil. Malays. Appl. Biol. 51(3):25–35, https://doi.org/10.55230/mabjournal.v51i3.2190
Bello-Gil, D., Roig-Molina, E., Fonseca, J., et al. (2018). An enzymatic system for decolorization of wastewater dyes using immobilized CueO laccase-like multicopper oxidase on poly-3-hydroxybutyrate. Microb. Biotechnol. 11(5):881–892, https://doi.org/10.1111/1751-7915.13287
Bilal, M., Adeel, M., Rasheed, T., Zhao, Y., and Iqbal, H.M.N. (2019). Emerging contaminants of high concern and their enzyme-assisted biodegradation. Environ. Int. 124:336–353, https://doi.org/10.1016/j.envint.2019.01.011
Chopra, N.K., and Sondhi, S. (2022). Cloning, expression and characterization of laccase from Bacillus licheniformis NS2324 in Escherichia coli and its application in dye decolorization. Int. J. Biol. Macromol. 206:1003–1011, https://doi.org/10.1016/j.ijbiomac.2022.03.104

Fernández-Fernández, M., Sanromán, M.A., and Moldes, D. (2013). Recent developments and applications of immobilized laccase. Biotechnol. Adv. 31:1808–1825, https://doi.org/10.1016/j.biotechadv.2012.02.013
Garzón-Posse, F., Becerra-Figueroa, L., Hernández-Arias, J., and Gamba-Sánchez, D. (2018). Whole cells as biocatalysts in organic transformations. Molecules 23(6):1265, https://doi.org/10.3390/molecules23061265
Grass, G., and Rensing, C. (2001). CueO is a multi-copper oxidase that confers copper tolerance in Escherichia coli. Biochem. Biophys. Res. Commun. 286(5):902–908, https://doi.org/10.1006/bbrc.2001.5474
Guo, H., Zheng, B., Jiang, D., and Qin, W. (2017). Overexpression of a laccase with dye decolorization activity from Bacillus sp. induced in Escherichia coli. J. Mol. Microbiol. Biotechnol. 27(4):217–227, https://doi.org/10.1159/000478859
Hammari, A.M., Abubakar, S., and Aisha, A.N. (2023). Bioremediation of methylene blue dye by a soil bacterium. J. Environ. Bioremediat. Toxicol. 6(2):43–46, https://doi.org/10.54987/jebat.v6i2.983
Ihssen, J., Reiss, R., Luchsinger, R., Thony-Meyer, L., and Richter, M. (2015). Biochemical properties and yields of diverse bacterial laccase-like multicopper oxidases expressed in Escherichia coli. Sci. Rep. 5:10465, https://doi.org/10.1038/srep10465
Kudanga, T., Nemadziva, B., and Le Roes-Hill, M. (2017). Laccase catalysis for the synthesis of bioactive compounds. Appl. Microbiol. Biotechnol. 101:13–33, https://doi.org/10.1007/s00253-016-7987-5
Kunamneni, A., Plou, F.J., Ballesteros, A., and Alcalde, M. (2008). Laccases and their applications: A patent review. Recent Pat. Biotechnol. 2(1):10–24, https://doi.org/10.2174/187220808783330965
Martins, L.O., Durão, P., Brissos, V., and Lindley, P.F. (2015). Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology. Cell. Mol. Life Sci. 72:911–922, https://doi.org/10.1007/s00018-014-1822-x
Mate, D.M., and Alcalde, M. (2016). Laccase: a multi-purpose biocatalyst at the forefront of biotechnology. Microb. Biotechnol. 10:1457–1467, https://doi.org/10.1111/1751-7915.12422
Niinimaki, K., Peters, G., Dahlbo, H., Perry, P., Rissanen, T., and Gwilt, A. (2020). The environmental price of fast fashion. Nat. Rev. Earth Environ. 1:189–200, https://doi.org/10.1038/s43017-020-0039-9
Outten, F.W., Huffman, D.L., Hale, J.A., and O’Halloran, T.V. (2001). The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli. J. Biol. Chem. 276(33):30670–30677, https://doi.org/10.1074/jbc.M104122200
Pham, V.H., Kim, J., Chang, S., and Chung, W. (2022). Biodegradation of methylene blue using a novel lignin peroxidase-producing bacterium (Bacillus sp. React3). Fermentation 8(4):190, https://doi.org/10.3390/fermentation8050190
Saratale, R.G., Saratale, G.D., Chang, J.S., and Govindwar, S.P. (2011). Bacterial decolorization and degradation of azo dyes: A review. J. Taiwan Inst. Chem. Eng. 42(1):138–157, https://doi.org/10.1016/j.jtice.2010.06.006
Wang, W., Zhang, Z., Ni, H., et al. (2012). Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase. Microb. Cell Fact. 11:75, https://doi.org/10.1186/1475-2859-11-75
Wu, K., Shi, M., Pan, X., et al. (2022). Decolourization and biodegradation of methylene blue by a ligninolytic enzyme-producing Bacillus thuringiensis. Enzyme Microb. Technol. 156:109999, https://doi.org/10.1016/j.enzmictec.2022.109999
Xi, Y., Shen, Y., Yang, F., et al. (2013). Removal of azo dye from aqueous solution by a new biosorbent prepared with Aspergillus nidulans. J. Taiwan Inst. Chem. Eng. 44(5):815–820, https://doi.org/10.1016/j.jtice.2013.01.031
Zammuto, V., Macrì, A., Agostino, E., et al. (2024). Enhancement of biodegradation and detoxification of methylene blue by preformed biofilm of thermophilic bacilli. J. Mar. Sci. Eng. 12(8):1248, https://doi.org/10.3390/jmse12081248

Details

Primary Language

English

Subjects

Conservation and Biodiversity

Journal Section

Research Article

Authors

Şeyma Akkurt ^*
0000-0002-0135-1975
Türkiye

Publication Date

July 1, 2026

Submission Date

February 10, 2026

Acceptance Date

March 16, 2026

Published in Issue

Year 2026 Volume: 2 Number: 1

IZ

https://izlik.org/JA29DE48PR

Cite

RIS / Bibtex

APA

Akkurt, Ş. (2026). Recombinant Bacterial Bioremediation of Synthetic Dye Pollution as a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation. Forest and Wood Science, 2(1), 1-6. https://izlik.org/JA29DE48PR

AMA

1.Akkurt Ş. Recombinant Bacterial Bioremediation of Synthetic Dye Pollution as a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation. FOWS. 2026;2(1):1-6. https://izlik.org/JA29DE48PR

Chicago

Akkurt, Şeyma. 2026. “Recombinant Bacterial Bioremediation of Synthetic Dye Pollution As a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation”. Forest and Wood Science 2 (1): 1-6. https://izlik.org/JA29DE48PR.

EndNote

Akkurt Ş (July 1, 2026) Recombinant Bacterial Bioremediation of Synthetic Dye Pollution as a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation. Forest and Wood Science 2 1 1–6.

IEEE

[1]Ş. Akkurt, “Recombinant Bacterial Bioremediation of Synthetic Dye Pollution as a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation”, FOWS, vol. 2, no. 1, pp. 1–6, July 2026, [Online]. Available: https://izlik.org/JA29DE48PR

ISNAD

Akkurt, Şeyma. “Recombinant Bacterial Bioremediation of Synthetic Dye Pollution As a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation”. Forest and Wood Science 2/1 (July 1, 2026): 1-6. https://izlik.org/JA29DE48PR.

JAMA

1.Akkurt Ş. Recombinant Bacterial Bioremediation of Synthetic Dye Pollution as a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation. FOWS. 2026;2:1–6.

MLA

Akkurt, Şeyma. “Recombinant Bacterial Bioremediation of Synthetic Dye Pollution As a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation”. Forest and Wood Science, vol. 2, no. 1, July 2026, pp. 1-6, https://izlik.org/JA29DE48PR.

Vancouver

1.Şeyma Akkurt. Recombinant Bacterial Bioremediation of Synthetic Dye Pollution as a Nature-Based Strategy for Aquatic Ecosystem and Biodiversity Conservation. FOWS [Internet]. 2026 Jul. 1;2(1):1-6. Available from: https://izlik.org/JA29DE48PR