LOKAL OTOJEN OSTEOKONDRAL GREFT UYGULAMALARI: KÖPEKLERDE DİZ EKLEMİNDE DENEYSEL ÇALIŞMA

Öz

Yapılan bu çalışmanın amacı köpeklerde diz eklemindeki osteokondral defektlerin onarımında lokal otojen osteokondral greftlerin uygulanabilirliğini araştırmaktı.

 

Çalışmada 18 adet sağlıklı, yetişkin melez köpek kullanıldı. Osteokondral defektler, standart silindir biçimin-de (0.5 cm x 0.5 cm) femurun lateral ve medial kondyluslarının her ikisinde de oluşturuldu. Oluşturulan defektler 9 köpekte (tedavi grubu) femoral trochlea’nın lateral ve medial kısımlarının üst yüzeylerinden elde edilen otojen osteokondral greftlerle dolduruldu. Dokuz köpek ise kontrol grubu olarak tutuldular.

 

Klinik ve radyolojik kontroller 15 gün aralıklarla gerçekleştirildi. Histopatolojik kontroller ise 15, 30 ve 60. günlerde gerçekleştirildi.

 

Sonuç olarak, bulgular köpeklerin diz eklemindeki osteokondral defektlerin, lateral ve medial femoral trochlea’nın üst kısımlarından elde edilen otojen osteokondral greftlerle sağaltımlarının gerçekleştirilebilece-ğini göstermiştir.

Anahtar Kelimeler

• Otojen Osteokondral Greft,Köpek

LOCAL AUTOGENOUS OSTEOCHONDRAL GRAFT APPLICATIONS: AN EXPERIMENTAL STUDY IN DOGS KNEE JOINTS

Abstract

The aim of this study was to investigate applicability of local autogenous osteochondral grafts for the repairment of osteochondral defects in knee joints of dogs.

 

The material consisted of 18 healthy adult mongrel dogs. Osteochondral defects in standard cylindrical shape (0.5 cm x 0.5 cm) were created on both lateral and medial femoral condyles.The defects were filled with autogenous osteochondral grafts harvested from upper surface of lateral and medial labium of femoral trochlea in treatment group (9 dogs). Nine dogs were kept as control group.

 

Clinical and radiographical evaluations were carried out at the 15 days intervals. Histopathological examinations were carried out at the 15^th, 30^th and 60^th days in each group.

 

Consequently, the results indicated that autogenous osteochondral grafts harvested from upper surface of lateral and medial labium of femoral trochlea could be applied in treatment of osteochondral defects in knee joints of dogs.

Keywords

• Autogenous Osteochondral Graft,Dog

References

1. 1. Delcogliano A., Caporaso A., Menghi A., Rinonapoli G. and Chiossi S. 2002. Results of autologous osteochondral grafts in chondral lesions of the knee. Minerva Chirurgica, 57: 273-281.
2. 2. Desjardins M.R., Hurtig M.B., Palmer N.C. 1991. Incorporation of fresh and cryopreserved bone in osteochondral autografts in the horse. Vet. Surg., 20: 446-452.
3. 3. Dew T.L., Martin R.A. 1992. Functional, radiographic, and histologic assessment of healing of autogenous osteochondral grafts and full-thickness cartilage defects in the talus of dogs. Am. J. Vet. Res., 53: 2141-2152.
4. 4. Dew T.L., Martin R.A. 1991. The functional, radiographic and histologic comparison of an autogenous orthotopic osteochondral graft and a full-thickness cartilage defect in the canine talus. Vet. Surg., 20: 334.
5. 5. Fallon K.R. 1990. Osteochondritis Dissecans of the Stiffle. In: M.J. Bojrab (ed.): Current Techniques in Small Animal Surgery, Third Ed. Lea & Febiger, Philadelphia, 721-722.
6. 6. Hangody L., Kish G., Kárpáti Z., Szerb I., Udvarhelyi I. 1997. Arthroscopic autogenous osteochondral mosaicplasty for the treatment of femoral condylar articular defects. A preliminary report. Knee Surg. Sport Tr. A., 5: 262-267.
7. 7. Hangody L., Vásárhelyi G., Hangody L. R., Sükösd Z., Tibay G., Bartha L., Bodó G. 2008. Autologous osteochondral grafting-technique and long-term results. Injury, 39, Suppl.1:32-39.
8. 8. Howard R.D., McIlwraith C.W., Trotter G.W., Powers B.E., Mcfadden P.R, Harwood F.L., Amiel D. 1994. Long-term fate and effects of exercise on sternal cartilage autografts used for repair of large osteochondral defects in horses. Am. J. Vet. Res., 55: 1158-1167.

9. 9. Huber M.J., Schmotzer W.B., Riebold T.W., Watrous B.J., Synder S.P., Scott E.A., Matthiessen P.C., Von-Matthiessen P.C. 1992. Fate and effects of autogenous osteochondral fragments implanted in the middle carpal joint of horses. Am. J. Vet. Res., 53: 1579-1588.
10. 10. Hurtig M. Pearce S., Warren S., Kalra M., Miniaci A. 2001. Arthroscopic Mosaic Arthroplasty in the Equine Third Carpal Bone. Vet. Surg., 30: 228-239.
11. 11. Hurtig M.B. 1988. Experimental use of small osteochondral grafts for resurfacing the equine third carpal bone. Equine Vet. J., Suppl, 6: 23-27.
12. 12. Karataglis D., Green M.A., Learmonth D.J.A. 2006. Autologous osteochondral transplantation for the treatment of chondral defects of the knee. Knee, 13 : 32-35.
13. 13. Kold S.E., Hickman J. 1986. An experimental study of the healing process of equine chondral and osteochondral defects. Equine Vet. J., 18: 18-24.
14. 14. Mahadev A., Mahara D.P., Chang P., Mitra A.K., Tay B.K., Sim C.S. 2001. Autogenous osteochondral morselised grafts for full thickness osteochondral defects in the knee joints of pigs. Singapore Med. J., 42: 410-416.
15. 15. Manley P.A., Howard P.E., Constantinescu G.M. 1990. Surgery for Osteochondrosis of the Canine Shoulder. In: M.J. Bojrab (ed):, Current Techniques in Small Animal Surgery, Third Ed. Lea & Febiger, Philadelphia, 749-756.
16. 16. Marcacci M., Kon E., Zaffagnini S., Visani A. 1999. Use of autologous grafts for reconstruction of osteochondral defects of the knee. Orthopedics, 22: 595-600.
17. 17. McIlwraith C.W., Howard R.D., Trotter G.W., Powers B.E., McFadden P.R, Harwood F.L., Amiel D. 1994. Sternal cartilage autografts for repair of large osteochondral defects in horses: long-term fate and effects of exercise. Vet. Comp. Orthopaed., 7: 63-64.
18. 18. Messner K., Gillquist J. 1993. Synthetic implant for the repair of osteochondral defects of the medial femoral condyle. Biomaterials, 14: 513-521.
19. 19. Oka Y., Ikeda M. 2001. Treatment of severe osteochondritis dissecans of the elbow using osteochondral grafts from a rib. J. Bone Joint Surg. Br., 83: 738-739.
20. 20. Piermattei D.L., Greeley R.G. 1979. An Atlas of Surgical Approaches to the Bones of the Dog and Cat, 202 pages, WB Saunders Company, Philadelphia.
21. 21. Sammarco G.J., Makwana N.K. 2002. Treatment of talar osteochondral lesions using local osteochondral graft. Foot Ankle Int., 23: 693-698.
22. 22. Scranton P.E., McDermott J.E. 2001. Treatment of type v osteochondral lesions of the talus with ipsilateral knee osteochondral autografts. Foot Ankle Int., 22: 380-384.
23. 23. Shahgaldi B.F. 1998. Coral graft restoration of osteochondral defects. Biomaterials, 19: 205-213.
24. 24. Stevenson S. 1986. Histomorphometric analysis of massive osteochondral allografts in dogs. Vet. Surg., 15. 135.
25. 25. Stover S.M., Pool R.R., Lloyd K.C.K. 1989. Repair of surgically created osteochondral defects with autogenous sternal osteochondral grafts in the horse. Vet. Surg., 18: 76-77.
26. 26. Sullins K.E., Veıt H.P., McIlwraith C.W. 1989. Osteochondral grafts to fill large articular defects in horses. Vet. Surg., 18: 77.
27. 27. Vachon A.M., McIlwraith C.W., Powers B.E., McFadden P.R., Amiel D. 1992. Morphologic and biochemical study of sternal cartilage autografts for resurfacing induced osteochondral defects in horses. Am. J. Vet. Res., 53: 1038-1047.
28. 28. Vachon A.M., McIlwraith C.W., Trotter G.W., Norrdin R.W., Powers B.E. 1991. Morphologic study of induced osteochondral defects of the distal portion of the radial carpal bone in horses by use of glued periosteal autografts. Am. J. Vet. Res., 52: 317-327.
29. 29. Yaylaoğlu M.B., Yıldız C., Korkusuz F., Hasırcı V. 1999. A novel osteochondral implant. Biomaterials, 20: 1513-1520.
30. 30. Yıldız C., Bahçe M., Deveci S., Bilgili H., İde T., Tunay S., Başbozkurt M., Gür E. 2001. Autolog condrocyte transplantation (an experimental study in rabbit knee joints). Artroplasti Artroskopik Cerrahi, 12: 75-82.