HPLC analysis of monomers eluted from self-adhesive resin cements

Year 2016, , 109 - 14, 18.08.2016

Özgür Genç Şen , Evrim Eligüzeloğlu Dalkılıç Hacer Deniz Arısu , Hüma Ömürlü , Gürcan Eskitaşçıoğlu

https://doi.org/10.17214/aot.85670

Abstract

OBJECTIVE: The aim of this study was to determine the residual monomer leaching from two self-adhesive resin cements polymerized with Light Emitting Diode (LED) or halogen light curing unit.

MATERIALS AND METHOD: Clearfil SA (group A, n = 48) and BisCem (group B, n = 48) cements were inserted in plastic moulds. Each group was further divided into two subgroups. Specimens were light cured with LED light curing unit (LCU) in group A1 and group B1 and halogen LCU in group A2 and group B2 for 20 seconds. The following compounds released from the samples stored in distilled water were analyzed: triethylene glycol-dimethacrylate (TEGDMA) and bisphenol A glycidyl methacrylate (Bis-GMA). Analysis of substances was performed with the use of high performance liquid chromatography, after 1 hour and 24 hour incubation periods. Factorial experimental design and independent t-test was used for statistical analyses.

RESULTS: Self-adhesive resin cements released more Bis-GMA and TEGDMA when they were polymerized with LED LCUs (p<0.05). Difference between different time periods was not statistically significant (p>0.05). Clearfil SA cement released more Bis-GMA than BisCem (p<0.05). BisCem released more TEGDMA than Clearfil SA (p<0.05).

CONCLUSION: The results of this study showed that the quantity of Bis-GMA and TEGDMA leached from self-adhesive resin cements was influenced by the type of LCU and by the type of self-adhesive resin cement.

Keywords

Dental curing lights, HPLC, resin cements

Kendinden bağlanabilen rezin simanlardan salınan monomerlerin HPLC ile incelenmesi

Abstract

AMAÇ: Bu çalışmanın amacı, Light Emitting Diode (LED) veya halojen ışık kaynaklarıyla sertleştirilmiş iki kendinden bağlanabilen rezin simanın artık monomer salımını belirlemektir.

GEREÇ VE YÖNTEM: Clearfil SA (grup A, n = 48) ve BisCem (grup B, n = 48) simanlar plastik kalıplara yerleştirildi. Her grup kendi içinde ikiye ayrıldı. Grup A1 ve B1’deki örnekler LED, grup A2 ve B2’deki örnekler halojen ışık kaynaklarıyla polimerize edildi. Distile suda tutulan örneklerden salınan trietilen glikol-dimetakrilat (TEGDMA) ve bisfenol A glisidilmetakrilat (Bis-GMA) bileşikleri, 1 saat ve 24 saat enkübasyon sonrasında yüksek performanslı likit kromotografisi (HPLC) ile incelendi. İstatistiksel değerlendirmeler için faktöriyel deneysel dizayn ve t testleri kullanıldı.

BULGULAR: Kendinden bağlanabilen rezin simanlar LED ışık kaynaklarıyla sertleştirildiğinde daha fazla Bis-GMA ve TEGDMA monomeri saldılar (p<0.05). Zaman periyodları arasında istatistiksel olarak anlamlı bir fark bulunamadı (p>0.05). Clearfil SA siman BisCem'den daha fazla Bis-GMA saldı (p<0.05); BisCem ise Clearfil SA'dan daha fazla TEGDMA saldı (p<0.05).

SONUÇ: Bu çalışmanın bulguları, kendinden bağlanabilen rezin simanlardan salınan Bis-GMA ve TEGDMA miktarlarının ışık kaynağı ve rezin simanın tipinden etkilendiğini göstermiştir.

Keywords

Dental polimerize edici ışık kaynakları, HPLC, rezin simanları

References

• Hansel C, Leyhausen G, Mai UEH, Geurtsen W. Effects of various composite resins (co)monomers and extracts on two caries-associated microorganisms in vitro. J Dent Res 1998;77:60-7.
• Heil J, Reifferscheid G, Waldmann P, Leyhausen G, Geurtsen W. Genotoxicity of dental materials. Mutat Res 1996;368:181-94.
• Taira M, Toguchi MS, Takahasi J, Okazaki M. Cytotoxicity of three di-functional monomers used in dental composite resins. J Mater Sci Lett 2000;19:547-8.
• Yoshii E. Cytototoxic effects of acrylates and methacrylates: Relationships of monomer structures and cytotoxicity. J Biomed Mater Res 1997;37:517-24.
• Komurcuoglu E, Olmez S, Vural N. Evaluation of residual monomer elimination methods in three different fissure sealents in vitro. J Oral Rehabil 2005;32:116-21.
• Kwon HJ, Oh YJ, Jang JH, Park JE, Hwang KS, Park YD. The effect of polymerization conditions on the amounts of unreacted monomer and bisphenol A in dental composite resins. Dent Mater J 2015;34:327-35.
• Sideridou ID. Achilias DS. Elution study of unreacted Bis-GMA, TEGDMA, UDMA, and Bis-EMA from light-cured dental resins and resin composites using HPLC.J Biomed Mater Res B 2005;74:617-26.
• Altıntaş SH, Usumez A. HPLC analyses of HEMA released from two different adhesive systems. J Biomed Mater Res B 2009;91:924-9.
• Halvorson RH, Erickson RL, Davidson C. Energy dependent polymerization of resin-based composite. Dent Mater 2002;18:463-9.
• Cebe MA, Cebe F, Cengiz MF, Çetin AR, Arpag OF, Ozturk B. Elution of monomer from different bulk fill dental composite resins. Dent Mater 2015;31:141-9.
• Tanaka K, Taira M, Shintani H, Wakasa K, Yamaki M. Residual monomers (TEGDMA and Bis-GMA) of a set visible light-cured dental composite resin when immersed in water. J Oral Rehabil 1991;18:353- 62.
• Tarle Z, Meniga A, Knezevix A, Sutalo J, Risti XM, Pichler G. Composite conversion and temperature rise using a conventional, plasma arc, and an experimental blue LED curing unit. J Oral Rehabil 2002;29:662-7.
• Ceballos L, Fuentes MV,Tafalla H, Martínez A, Flores J, Rodríguez J. Curing effectiveness of resin composites at different exposure times using LED and Halogen lights. J Med Oral Pathol Oral Cir Bucal 2009;14:51-6.
• Camilotti V, Grullon PG, Mendonça MJ, D’Alpino PH, Gomes JC.Influence of different light curing units on the bond strength of indirect resin composite restorations.Braz Oral Res 2008;22:164-9.
• Franco EB, Santos PA, Mondelli RFL. The effect of different lightcuring units on tensile strength and microhardness of a composite resin. J Appl Oral Sci 2007;15:470-4.
• Mills RW, Jandt KD, Ashworth SH. Dental composite depth of cure Halogen and blue light emitting diode technology. Br Dent J 1999;186:388-91.
• Hammesfahr PD, O’Connor MT, Wang X. Light curing technology: Past, present and future. Compend Contin Educ Dent 2002;23:18-24.
• Dunn WJ, Bush AC. A comparison of polymerization by light-emitting diode and Halogen based light curing units. J Am Dent Assoc 2002;133:335-41.
• Ak AT, Alpoz AR, Bayraktar O, Ertugrul F. Monomer release from resin based dental materials cured with LED and Halogen lights. Eur J Dent 2010;4:34-40.
• Tabatabaee MH, Mahdavi H, Zandi S, Kharrazi MJ. HPLC analysis of eluted monomers from two composite resins cured with LED and Halogen curing lights. J Biomed Mater Res B 2009;88:191-6.
• Munksgaard EC, Peutzfeldt A, Asmussen E. Elution of TEGDMA and BisGMA from a resin and a resin composite cured with Halogen or plasma light. Eur J Oral Sci 2000;108:341-5.
• Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res 1998;41:474-80.
• Schmalz G. The biocompatibilty of non-amalgam dental filling materials. Eur J Oral Sci 1998;106:696-706.
• Jorge JH, Giampaolo ET, Machado AL, Vergani CE. Cytotoxicity of denture base acrylic resins: A literature review. J Prosthet Dent 2003;90:190-3.
• Radovic I, Vulicevic ZR. Self-adhesive resin cements. Ferrari M, ed. Fiber posts and endodontically treated teeth. Wendywood: Modern Dentistry Media; 2008. p.107-19.