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The effects of ethylenediamine tetraacetic acid, peracetic acid, and etidronic acid on the tissue dissolution capacity of sodium hypochlorite: in vitro

Year 2017, Volume: 34 Issue: 2, 50 - 54, 15.03.2017
https://doi.org/10.17214/gaziaot.277456

Abstract

Objective: The aim of this study was to evaluate the effects of 18% ethylenediamine tetraacetic acid (EDTA), 2% peracetic acid (PAA), and 9% etidronic acid (HEBP) on the organic tissue dissolution activity of sodium hypochlorite (NaOCl).

Materials and Method: Sixty samples with similar weight and dimensions were obtained from bovine muscle tissue. The tissue samples were blotted dry on filter paper and weighed with a precision balance. The specimens were immersed in following solutions: (1) 2 mL 2.5% NaOCl, (2) 1 mL 5% NaOCl + 1 mL 18% EDTA, (3) 1 mL 5% NaOCl + 1 mL 2% PAA, (4) 1 mL 5% NaOCl + 1 mL 9% HEBP. The specimens were then dried and weighed again. The weight loss of each specimen incubated in the test solutions was measured at 30 and 60 min. The data were statistically analyzed with one-way ANOVA and post-hoc Tukey tests.

Results: Use of NaOCl (5%) together with 18% EDTA resulted in minimal tissue dissolution capacity compared to the other groups at both time points (p<0.001). The tissue dissolution capacity of NaOCl was also affected by 9% HEBP. The greatest tissue weight reduction values were obtained in the NaOCl+PAA group at 30 minutes (p<0.001). At 60 min, NaOCl and NaOCl+PAA groups exhibited the greatest tissue dissolution capacity (p<0.001); no significant difference was found between these two groups (p=0.169).

Conclusion: EDTA and HEBP decreased the tissue dissolution capacity of NaOCl, whereas PAA did not have any negative effect on the ability of NaOCl to dissolve the organic tissue.

References

  • Moorer W, Wesselink P. Factors promoting the tissue dissolving capacity of sodium hypochlorite. Int Endod J 1982;15:187-96.
  • Retamozo B, Shabahang S, Johnson N, Aprecio R, Torabinejad M. Minimum contact time and concentration of sodium hypochlorite required to eliminate enterococcus faecalis. J Endod 2010;36:520-3.
  • Lester KS, Boyde A. Scanning electron microscopy of instrumented, irrigated and filled root canals. Br Dent J 1977;143:359-67.
  • De-Deus G, Souza EM, Marins JR, Reis C, Paciornik S, Zehnder M. Smear layer dissolution by peracetic acid of low concentration. Int Endod J 2011;44:485-90.
  • Lottanti S, Gautschi H, Sener B, Zehnder M. Effects of ethylenediaminetetraacetic, etidronic and peracetic acid irrigation on human root dentine and smear layer. Int Endod J 2009;42:335-43.
  • Grawehr M, Sener B, Waltimo T, Zehnder M. Interactions of ethylenediamine tetraacetic acid with sodium hypochlorite in aqueous solutions. Int Endod J 2003;36:411-5.
  • Zehnder M, Schmidlin P, Sener B, Waltimo T. Chelation in root canal therapy reconsidered. J Endod 2005;31:817-20.
  • Girard S, Paqué F, Badertscher M, Sener B, Zehnder M. Assessment of a gel-type chelating preparation containing 1-hydroxyethylidene-1, 1-bisphosphonate. Int Endod J 2005;38:810-6.
  • McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev 1999;12:147-79.
  • Tanomaru-Filho M, Silveira BR, Martelo RB, Guerreiro-Tanomaru JM. Influence of Concentration and Agitation of Sodium Hypochlorite and Peracetic Acid Solutions on Tissue Dissolution. J Contemp Dent Pract 2015;16:876-9.
  • Zehnder M. Root canal irrigants. J Endod 2006;32:389-98.
  • De-Deus G, Zehnder M, Reis C, Fidel S, Fidel RA, Galan J Jr, et al. Longitudinal co-site optical microscopy study on the chelating ability of etidronate and EDTA using a comparative single-tooth model. J Endod 2008;34:71-5.
  • Camps J, Pommel L, Aubut V, Verhille B, Satoshi F, Lascola B, et al. Shelf life, dissolving action, and antibacterial activity of a neutralized 2.5% sodium hypochlorite solution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e66–73.
  • Jungbluth H, Marending M, De-Deus G, Sener B, Zehnder M. Stabilizing sodium hypochlorite at high ph: effects on soft tissue and dentin. J Endod 2011;37:693–6.
  • Kühlfluck I, Klammt J. Suitability of peracetic acid for root canal disinfection. Stomatol DDR 1980;30:558-63.
  • Ballal NV, Mala K, Bhat KS. Effect of maleic acid and ethylenediaminetetraacetic acid on the dissolution of human pulp tissue-an in vitro study. Int Endod J 2011;44:353-6.
  • Cobankara FK, Ozkan HB, Terlemez A. Comparison of organic tissue dissolution capacities of sodium hypochlorite and chlorine dioxide. J Endod 2010;36:272-4.
  • Dutta A, Saunders WP. Comparative evaluation of calcium hypochlorite and sodium hypochlorite on soft-tissue dissolution. J Endod 2012;38:1395-8.
  • Aubut V, Pommel L, Verhille B, Orsiere T, Garcia S, About I, et al. Biological properties of a neutralized 2.5% sodium hypochlorite solution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e120-5.
  • Slutzky-Goldberg I, Hanut A, Matalon S, Baev V, Slutzky H. The effect of dentin on the pulp tissue dissolution capacity of sodium hypochlorite and calcium hydroxide. J Endod 2013;39:980-3.

Etilendiamin tetraasetik asit, perasetik asit ve etidronik asitin sodyum hipokloritin doku çözme kapasitesi üzerine etkisi: in vitro

Year 2017, Volume: 34 Issue: 2, 50 - 54, 15.03.2017
https://doi.org/10.17214/gaziaot.277456

Abstract

Amaç: Bu çalışmanın amacı %18 etilendiamin tetraasetik asit (EDTA), %2 perasetik asit (PAA) ve %9 etidronik asit (HEBP) solüsyonlarının, sodyum hipokloritin (NaOCl) organik doku çözücü aktivitesi üzerindeki etkisinin değerlendirilmesidir.

Gereç ve Yöntem: Dana kas dokusundan benzer ağırlık ve boyutta 60 adet örnek elde edildi. Doku örnekleri gözenekli kağıt üzerinde kurutuldu ve hassas tartı ile tartıldı. Örnekler sırasıyla: (1) 2 mL %2.5 NaOCl, (2) 1 mL %5 NaOCl + 1 mL %18 EDTA, (3) 1 mL %5 NaOCl + 1 mL %2 PAA, (4) 1 mL %5 NaOCl + 1 mL %9 HEBP solüsyonlarına daldırıldı. Daha sonra örnekler kurutuldu ve tekrar tartıldı. Test solüsyonlarında bekletilen her örneğin ağırlık kaybı 30. ve 60. dakikalarda ölçüldü. Veriler, one-way ANOVA ve post-hoc Tukey testleri ile istatiksel olarak analiz edildi

Bulgular: Sodyum hipoklorit (%5) ve %18 EDTA’nın birlikte kullanımı, her iki zaman diliminde de diğer gruplarla karşılaştırıldığında en az doku eritme kapasitesine sahipti. (p<0.001). Etidronik asit (%9) solüsyonu kullanımı da, NaOCl’nin doku çözücü kapasitesini azalttı. En yüksek doku ağırlık düşüş değerleri 30. dakikada NaOCl + PAA grubundan elde edildi (p<0.001). Altmış dakikanın sonunda ise en fazla doku çözücü etkiyi NaOCl ve NaOCl + PAA grupları gösterdi (p<0.001); bu iki grup arasında anlamlı bir fark yoktu (p=0.169).

Sonuç: EDTA ve HEBP, NaOCl’nin doku eritme kapasitesini düşürdü. PAA, NaOCl’nin organik doku çözme yeteneği üzerine olumsuz etki göstermedi.

References

  • Moorer W, Wesselink P. Factors promoting the tissue dissolving capacity of sodium hypochlorite. Int Endod J 1982;15:187-96.
  • Retamozo B, Shabahang S, Johnson N, Aprecio R, Torabinejad M. Minimum contact time and concentration of sodium hypochlorite required to eliminate enterococcus faecalis. J Endod 2010;36:520-3.
  • Lester KS, Boyde A. Scanning electron microscopy of instrumented, irrigated and filled root canals. Br Dent J 1977;143:359-67.
  • De-Deus G, Souza EM, Marins JR, Reis C, Paciornik S, Zehnder M. Smear layer dissolution by peracetic acid of low concentration. Int Endod J 2011;44:485-90.
  • Lottanti S, Gautschi H, Sener B, Zehnder M. Effects of ethylenediaminetetraacetic, etidronic and peracetic acid irrigation on human root dentine and smear layer. Int Endod J 2009;42:335-43.
  • Grawehr M, Sener B, Waltimo T, Zehnder M. Interactions of ethylenediamine tetraacetic acid with sodium hypochlorite in aqueous solutions. Int Endod J 2003;36:411-5.
  • Zehnder M, Schmidlin P, Sener B, Waltimo T. Chelation in root canal therapy reconsidered. J Endod 2005;31:817-20.
  • Girard S, Paqué F, Badertscher M, Sener B, Zehnder M. Assessment of a gel-type chelating preparation containing 1-hydroxyethylidene-1, 1-bisphosphonate. Int Endod J 2005;38:810-6.
  • McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev 1999;12:147-79.
  • Tanomaru-Filho M, Silveira BR, Martelo RB, Guerreiro-Tanomaru JM. Influence of Concentration and Agitation of Sodium Hypochlorite and Peracetic Acid Solutions on Tissue Dissolution. J Contemp Dent Pract 2015;16:876-9.
  • Zehnder M. Root canal irrigants. J Endod 2006;32:389-98.
  • De-Deus G, Zehnder M, Reis C, Fidel S, Fidel RA, Galan J Jr, et al. Longitudinal co-site optical microscopy study on the chelating ability of etidronate and EDTA using a comparative single-tooth model. J Endod 2008;34:71-5.
  • Camps J, Pommel L, Aubut V, Verhille B, Satoshi F, Lascola B, et al. Shelf life, dissolving action, and antibacterial activity of a neutralized 2.5% sodium hypochlorite solution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e66–73.
  • Jungbluth H, Marending M, De-Deus G, Sener B, Zehnder M. Stabilizing sodium hypochlorite at high ph: effects on soft tissue and dentin. J Endod 2011;37:693–6.
  • Kühlfluck I, Klammt J. Suitability of peracetic acid for root canal disinfection. Stomatol DDR 1980;30:558-63.
  • Ballal NV, Mala K, Bhat KS. Effect of maleic acid and ethylenediaminetetraacetic acid on the dissolution of human pulp tissue-an in vitro study. Int Endod J 2011;44:353-6.
  • Cobankara FK, Ozkan HB, Terlemez A. Comparison of organic tissue dissolution capacities of sodium hypochlorite and chlorine dioxide. J Endod 2010;36:272-4.
  • Dutta A, Saunders WP. Comparative evaluation of calcium hypochlorite and sodium hypochlorite on soft-tissue dissolution. J Endod 2012;38:1395-8.
  • Aubut V, Pommel L, Verhille B, Orsiere T, Garcia S, About I, et al. Biological properties of a neutralized 2.5% sodium hypochlorite solution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e120-5.
  • Slutzky-Goldberg I, Hanut A, Matalon S, Baev V, Slutzky H. The effect of dentin on the pulp tissue dissolution capacity of sodium hypochlorite and calcium hydroxide. J Endod 2013;39:980-3.
There are 20 citations in total.

Details

Primary Language Turkish
Subjects Dentistry
Journal Section Original Research Article
Authors

Özgür İlke Atasoy Ulusoy

İlke Gaye Savur This is me

Bülent Çelik

Publication Date March 15, 2017
Published in Issue Year 2017 Volume: 34 Issue: 2

Cite

APA Atasoy Ulusoy, Ö. İ., Savur, İ. G., & Çelik, B. (2017). Etilendiamin tetraasetik asit, perasetik asit ve etidronik asitin sodyum hipokloritin doku çözme kapasitesi üzerine etkisi: in vitro. Acta Odontologica Turcica, 34(2), 50-54. https://doi.org/10.17214/gaziaot.277456
AMA Atasoy Ulusoy Öİ, Savur İG, Çelik B. Etilendiamin tetraasetik asit, perasetik asit ve etidronik asitin sodyum hipokloritin doku çözme kapasitesi üzerine etkisi: in vitro. Acta Odontol Turc. March 2017;34(2):50-54. doi:10.17214/gaziaot.277456
Chicago Atasoy Ulusoy, Özgür İlke, İlke Gaye Savur, and Bülent Çelik. “Etilendiamin Tetraasetik Asit, Perasetik Asit Ve Etidronik Asitin Sodyum Hipokloritin Doku çözme Kapasitesi üzerine Etkisi: In Vitro”. Acta Odontologica Turcica 34, no. 2 (March 2017): 50-54. https://doi.org/10.17214/gaziaot.277456.
EndNote Atasoy Ulusoy Öİ, Savur İG, Çelik B (March 1, 2017) Etilendiamin tetraasetik asit, perasetik asit ve etidronik asitin sodyum hipokloritin doku çözme kapasitesi üzerine etkisi: in vitro. Acta Odontologica Turcica 34 2 50–54.
IEEE Ö. İ. Atasoy Ulusoy, İ. G. Savur, and B. Çelik, “Etilendiamin tetraasetik asit, perasetik asit ve etidronik asitin sodyum hipokloritin doku çözme kapasitesi üzerine etkisi: in vitro”, Acta Odontol Turc, vol. 34, no. 2, pp. 50–54, 2017, doi: 10.17214/gaziaot.277456.
ISNAD Atasoy Ulusoy, Özgür İlke et al. “Etilendiamin Tetraasetik Asit, Perasetik Asit Ve Etidronik Asitin Sodyum Hipokloritin Doku çözme Kapasitesi üzerine Etkisi: In Vitro”. Acta Odontologica Turcica 34/2 (March 2017), 50-54. https://doi.org/10.17214/gaziaot.277456.
JAMA Atasoy Ulusoy Öİ, Savur İG, Çelik B. Etilendiamin tetraasetik asit, perasetik asit ve etidronik asitin sodyum hipokloritin doku çözme kapasitesi üzerine etkisi: in vitro. Acta Odontol Turc. 2017;34:50–54.
MLA Atasoy Ulusoy, Özgür İlke et al. “Etilendiamin Tetraasetik Asit, Perasetik Asit Ve Etidronik Asitin Sodyum Hipokloritin Doku çözme Kapasitesi üzerine Etkisi: In Vitro”. Acta Odontologica Turcica, vol. 34, no. 2, 2017, pp. 50-54, doi:10.17214/gaziaot.277456.
Vancouver Atasoy Ulusoy Öİ, Savur İG, Çelik B. Etilendiamin tetraasetik asit, perasetik asit ve etidronik asitin sodyum hipokloritin doku çözme kapasitesi üzerine etkisi: in vitro. Acta Odontol Turc. 2017;34(2):50-4.