Electrochemical determination of ascorbic acid with thermally reduced graphene oxide

Year 2020, Volume: 35 Issue: 3, 1589 - 1602, 07.04.2020

Merve Okutan

https://doi.org/10.17341/gazimmfd.645284

Cited By: 2

Abstract

In this study, electrochemical behavior of a glassy
carbon electrode (GCE), which is covered with thermally reduced graphene oxide
(T-rGO) and Nafion solution (N117), is investigated using the cyclic
voltammetry (CV), differential pulse voltammetry (DPV) and amperometric
techniques in phosphate buffer solution (PBS) contains ascorbic acid (AA). For
this purpose, T-rGO is synthesized from synthetic graphite as the starting
material to graphene oxide by modified Hummers method, followed by reduction of
graphene oxide with flash heat treatment in inert gas environment. In order to
characterize the structure, several techniques such as SEM, XRD, Raman, FTIR and
elemental analysis are used. Number of layer of T-rGO is determined as about
3.32 with using XRD analysis data. In the PBS with different pH values (6, 7
and 8) containing 5.0 mM AA, the highest current value with a clearly anodic
oxidation peak is obtained for the GCE/T-rGO electrode in the pH 8 PBS.
According to the results obtained with GCE/T-rGO electrode, AA can be detected
over a wide linear detection range between the AA concentration and anodic peak
current (4.0-100.0 mM, R²=0.9978) with good sensitivity (0.3 μA mM^-1),
high detection limit (0.61 μM), good reproducibility (RSD=6.25%, n=3) and
repeatability (RSD=2.14%, n=3). In addition, the GCE/T-rGO electrode showed
good selectivity against the uric acid, NaCl and CaCl₂. For these
reasons, it is thought that the GCE/T-rGO electrodes can be used for
electrochemical determination of AA molecule.

Keywords

Reduced graphene oxide, modified hummers method, ascorbic acid, glassy carbon electrode

Termal indirgenmiş grafen oksit ile elektrokimyasal olarak askorbik asit tayini

Abstract

Bu çalışmada termal olarak indirgenmiş grafen
oksit (T-rGO) ve Nafion çözeltisi (N117) ile kaplanmış camsı karbon elektrotun
(GCE), fosfat tampon çözeltisindeki (PBS) askorbik asitin (AA) varlığına göre
elektrokimyasal davranışı dönüşümlü voltametri (CV), diferansiyel puls
voltametri (DPV) ve amperometrik tekniklerle incelenmiştir. Bu amaçla T-rGO,
başlangıç malzemesi olan sentetik grafitten modifiye Hummers yöntemi ile grafen
oksite (GO), ardından da GO’nun inert gaz ortamında termal işlem uygulanarak
indirgenmesi ile sentezlenmiştir. Yapının karakterize edilmesi amacıyla SEM,
XRD, Raman, FTIR ve elementel analiz gibi çeşitli teknikler kullanılmıştır. XRD
analiz verileri kullanılarak yaklaşık 3,32 tabaka sayısına sahip T-rGO elde
edildiği tespit edilmiştir. 5,0 mM AA içeren farklı pH değerlerine sahip (6, 7
ve 8) PBS arasından, pH 8 çözeltisindeki GCE/T-rGO elektrotu için en belirgin
anodik yükseltgenme piki elektrot üzerinden geçen en yüksek akım miktarıyla elde
edilmiştir. GCE/T-rGO elektrotu ile elde edilen sonuçlara göre, AA, derişim ve
anodik pik akımı arasındaki geniş doğrusal tayin aralığında (4,0-100,0 mM, R²=0,9978)
yüksek hassasiyet (0,3 μA mM^-1), tayin limiti (0,61 μM), yeniden
üretilebilirlik (RSD=%6,25, n=3) ve tekrarlanabilirlikle (RSD=%2,14, n=3)
belirlenebilmektedir. Ayrıca, GCE/T-rGO elektrot ürik asit, NaCl ve CaCl₂’e
karşı yüksek seçicilik sergilemiştir. Bu nedenlerle, hazırlanan GCE/T-rGO
elektrotların
AA molekülünün elektrokimyasal tayininde kullanılabileceği düşünülmektedir.

Keywords

İndirgenmiş grafen oksit, modifiye hummers yöntemi, askorbik asit, camsı karbon elektrot

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