R. delemar ’IN ASİT FOSFATAZ ÜRETİMİ ve Zn(II) BİYOBİRİKİMİNİN CEVAP YÜZEY YÖNTEMİ KULLANARAK OPTİMİZE EDİLMESİ
Abstract
R. delemar’ ın asit fosfataz aktivitesi, Zn(II) biyobirikimi ve üremesi, pH, sıcaklık ve başlangıç metal iyonu konsantrasyonunun fonksiyonu olarak araştırıldı. Deney tasarımı Cevap Yüzey Yöntemi (CYY) ve Box-Behnken tasarımına göre yapıldı. Önerilen matematiksel modeller varyans analizi (ANNOVA) istatistiksel yöntemi ile değerlendirilmiştir. Asit fosfataz aktivitesi için doğrusal, mikroorganizma derişimi ve Zn(II) biyobirikimi için kuadratik eşitliğe tam bir uyum gözlenmiştir. R. delemar’ ın üremesi asit fosfataz üretimi ve Zn(II) biyobirikimi için optimum pH 5,03, sıcaklık 31,82 oC ve başlangıç Zn(II) iyonu konsantrasyonu 30,15 mg/L olarak bulunmuştur. Bu koşullarda elde edilen maksimum asit fosfataz aktivitesi 794,098 µmol/L-dak, mikroorganizma konsantrasyonu 3,203 g/L ve biyobiriktirilen Zn(II) miktarı 17,62 mg/L olarak bulunmuştur. Deney doğrulama deneyi yapılarak sonuçların duyarlılığı ve geçerliliği gösterilmiştir.
References
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Year 2014,
Volume: 29 Issue: 2, 0 - , 16.06.2014
Abstract
References
- Abdallah A.A, Magboul P., L.H. McSweeney. ‘Department of food science and techonology’, Food chemistry, 6515-6522, 1999.
- Schmidt G.G. Bartsch. M. C. Lamont. T. Herman. and M. Liss, ‘ Acid phosphatase of baker's yeast: an enzyme of the external cell surface’, Biochemistry, 2, 126-121, 1963.
- Nyc. J.F. ‘A repressible acid phosphatase in Neurospora crassa’, Biochem. Biophysic, 27, ,183-188, 1967.
- Blum J. J. ‘Observation of the acid phosphatases of Euglena gracilis’, J. Cell Biology., 24, 223-234, 1956.
- Tsekova K. and Galabova D. ‘Phosphatase production and activity in copper(II) accumulating Rhizopus delemar’. Enzyme Microb. Technology, 33, 926–931, 2003.
- Leborans G.F. and Novillo. A., “Toxicity and Bioaccumulation of Cadmium Olisthodiscus luteus (Raphidophyceae) Water Resource, 30, 57-62, 1996.
- Tsezos M. and Volesky B., “Biosorption of Uranium and Thorium”. Biotechnology and Bioengineering, 23, 583-604, 1981.
- Macaskie L.E., Wates J.M., Dean A.C.R.’ Cadmium accumulation by a Citrobacter sp. immobilized on gel and solid supports: applicability to the treatment of liquid wastes containing heavy metal cations’, Biotechnology and Bioengineering, 30, 66-73, 1987.
- Stat ease, Inc 2021 East Hannepin Ave., Suite 480 Minneapolis, MN 55413 Design Expert version 7.0 , Buil date 2005.
- Acıkel Ü., Ersan, M., ‘Acid phosphatase production by Rhizopus delemar: A role played in the Ni(II) bioaccumulation process’, Journal of Hazardous Materials, 184, 632-639, 2010.
- Durak Z. E, Gürü M., 'Isolation, Inhıbition and Kinetic Modelling of alakline phospatase (Alp) Enzyme'Journal of Faculty of Engineering and Architecture of Gazi University., 28, 1, 2013.
There are 11 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Makaleler |
| Authors | |
| Publication Date | June 16, 2014 |
| Submission Date | June 16, 2014 |
| Published in Issue | Year 2014 Volume: 29 Issue: 2 |