Insecticidal and Antimicrobial Effects of Pseudomonas Species Isolated From Waste Water

Abstract

In this study, the insecticidal effects of Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida isolated from refinery wastewater on Leptinotarsa decemlineata and Dendroctonus micans larvae and antimicrobial effects against different microorganisms were investigated. L. decemlineata and D. micans larvae collected from the Kümbet Plateau of Giresun province were fed with isolate inoculated leaf and bark samples. At the end of 7 days of application, the viability and mortality rates were calculated. Antimicrobial activities of isolates against gram negative, gram positive and fungal species were investigated by disc diffusion method using fermentation broths. As result, at the end of the 7-day application, a mortality rate of 69-85% was obtained for L. decemlineata larvae. On the other hand, 49-78% mortality was obtained in D.micans larvae. This finding indicates that D. micans is more sensitive to isolates. It was determined that the isolates exhibited different levels of antimicrobial activity against gram negative, gram positive and Candida species. These results show that Pseudomonas species isolated from refinery wastewater have high potential in biological control against L. decemlineata and D. micans and exhibit broad-spectrum antimicrobial activity.

Keywords

• Biological control
• Dendroctonus micans
• Leptinotarsa decemlineata
• Pseudomonas sp.

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