VAUCHERIA FRIGIDA (ROTH) C. AGARDH’IN ANTİOKSİDAN VE ANTİBAKTERİYEL ÖZELLİKLERİ

Abstract

Bu çalışmada, Dicle Nehri Diyarbakır il sınırları içindeki bentik habitatlardan izole edilen Vaucheria frigida (Roth) C.Agardh 1824 (sinonim Conferva frigida Roth 1797)’nın antioksidan ve antibakteriyel özellikleri incelenmiş, total fenolik ve flavonoid oranlarına da bakılmıştır. Genellikle tatlı su ekosistemlerinde ve litoral bölgedeki epilitik habitatta yaşayan V. frigida’nın total fenolik madde miktarı 42.81±2.79 mg GAE/gEkstre ve total flavonoid madde miktarı ise 3.62±2.81 mg QE/gEkstre olarak tespit edilmiştir. Antioksidan analizlerde DPPH (>500 µg/ml) ve ABTS aktivitesi (186.26 µg/ml) Trolox ile karşılaştırıldığında daha az oranda olduğu görülmüştür. FRAP için de durum benzer şekilde düşük (27.5 mgTE/gEkstre) olarak ölçülmüştür. Antibakteriyel analizlerde V. frigida ' nın Etanol1(E1) ve Etanol2 (E2) ekstraktlarının, S. aureus, E. coli, K. pneumoniae, A. baumannii bakterilerilerine karşı antibakteriyel aktiviteleri disk difüzyon yöntemiyle incelenmiştir. Antibakteriyel aktivite testlerinde E1 ekstresi için en duyarlı organizma K. pneumoniae (14.53±0.5 mm), en dirençli organizma ise S. aureus (9.23±0.35 mm) olmuştur. E2 ekstresi için ise, E.coli (14.37±0.21 mm) en duyarlı, A.baumanii (11,10±0.1 mm) ise en dirençli organizma olarak kaydedilmiştir. Sonuç olarak, V. frigida’ nın, gelecekte yapılacak farmasötik çalışmalarda kullanılabilecek biyoaktif bir organizma potansiyeline sahip olduğu tespit edilmiştir.

Keywords

• Vaucheria frigida
• Conferva frigida
• Dicle Nehri(Diyarbakır)
• Antioksidan
• Antibakteriyel

ANTIOXIDANT AND ANTIBACTERIAL PROPERTIES OF VAUCHERIA FRIGIDA (ROTH) C. AGARDH

Abstract

In this study, the antioxidant and antibacterial properties of Vaucheria frigida (Roth) C. Agardh 1824 (synonym Conferva frigida Roth 1797), isolated from benthic habitats within the Tigris River Diyarbakır provincial borders, were examined, and total phenolic and flavonoid ratios were also examined. The total phenolic substance amount of V. frigida, which generally lives in freshwater ecosystems and epilithic habitat in the littoral region, was determined as 42.81±2.79 mg GAE/gExtract and the total flavonoid substance amount was 3.62±2.81 mg QE/gExtract. In antioxidant analysis, DPPH (>500 µg/ml) and ABTS activity (186.26 µg/ml) were found to be lower compared to Trolox. For FRAP, the situation was measured to be similarly low (27.5 mgTE/gExtract). In antibacterial analyses, the antibacterial activities of Ethanol1 (E1) and Ethanol2 (E2) extracts of V. frigida against S. aureus, E. coli, K. pneumoniae, A. baumannii bacteria were examined by disk diffusion method. In antibacterial activity tests, the most sensitive organism for E1 extract was K. pneumoniae (14.53±0.5 mm), and the most resistant organism was S. aureus (9.23±0.35 mm). For E2 extract, E.coli (14.37±0.21 mm) was recorded as the most sensitive organism and A.baumanii (11.10±0.1 mm) was recorded as the most resistant organism. As a result, it has been determined that V. frigida has the potential to be a bioactive organism that can be used in future pharmaceutical studies.

Keywords

• Vaucheria frigida
• Conferva frigida
• Tigrid River(Diyarbakır)
• Antioxidant
• Antibacterial

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