Phytochemical screening and radical scavenging activity of green coffee bean extract: Multiple-protective efficacy against Bisphenol A

Year 2021, Volume: 10 Issue: 3, 256 - 275, 31.12.2021

Burhan Durhan Emine Yalçın , Kültigin Çavuşoğlu , Ali Acar

Abstract

In this study, phytochemical characterization, radical scavenging activity and the protective role of green coffee bean extract (Gcbe) against Bisphenol-A (BpA) were evaluated. In phytochemical analysis total phenolic, flavonoid and tannin contents were investigated and major components were determined by HPLC. The amerolative effect of Gcbe against BpA-induced toxicity were studied in albino mice. For this purpose, mice were separated into six groups which treated with tap-water, BpA and two doses of Gcbe. To determine BpA toxicity and the protective role of Gcbe, changes in some biochemical parameters, micronucleus (MN) frequency and DNA fragmentation were investigated. As a result, the phenolic compounds in the Gcbe content were more intense than the other components. In BpA-treated group, serum parameters increased and the antioxidant/oxidant dynamics were impaired. Gcbe treatment decreased the induced serum parameters and caused an improvement in antioxidant-oxidant dynamics and also a decrease in the DNA-tail percentage and MN frequency.

Bisphenol A, Comet, Green coffee bean extract, Phytochemical characterization, Radical scavenging activity

Keywords

Bisphenol A, Comet, Grren Coffee bean extract, phytochemical characterization, radical scavenging activity

Project Number

Giresun Üniversitesi Araştırma Fonu- FEN-BAP-C-200515-18

Phytochemical screening and radical scavenging activity of green coffee bean extract: Multiple-protective efficacy against Bisphenol A

Abstract

In this study, phytochemical characterization, radical scavenging activity and the protective role of green coffee bean extract (Gcbe) against Bisphenol-A (BpA) were evaluated. In phytochemical analysis total phenolic, flavonoid and tannin contents were investigated and major components were determined by HPLC. The amerolative effect of Gcbe against BpA-induced toxicity were studied in albino mice. For this purpose, mice were separated into six groups which treated with tap-water, BpA and two doses of Gcbe. To determine BpA toxicity and the protective role of Gcbe, changes in some biochemical parameters, micronucleus (MN) frequency and DNA fragmentation were investigated. As a result, the phenolic compounds in the Gcbe content were more intense than the other components. In BpA-treated group, serum parameters increased and the antioxidant/oxidant dynamics were impaired. Gcbe treatment decreased the induced serum parameters and caused an improvement in antioxidant-oxidant dynamics and also a decrease in the DNA-tail percentage and MN frequency.

Keywords

Bisphenol A, Comet, Green coffee bean extract, phytochemical characterization, radical scavenging activity

Supporting Institution

Giresun Üniversitesi-BAP

Project Number

Giresun Üniversitesi Araştırma Fonu- FEN-BAP-C-200515-18

References

• Allard, P., Colaiacovo, M. P., 2010. Bisphenol A impairs the double-strand break repair machinery in the germline and causes chromosome abnormalities. Proceedings of the National Academy of Sciences of the United States of America, 107(47), 20405-20410.
• Alristina, A. D., Adi, A. C., Ismawati, R., 2018. The effect of green coffee bean extract on the weight rats with high fat diet. Health Notions, 2(4), 446-449.
• Anet, A., Olakkaran, S., Purayil, A. K., Puttaswamygowda, G. H., 2019. Bisphenol A induced oxidative stress mediated genotoxicity in Drosophila melanogaster. The Journal of Hazardous Materials, 370, 42-53.
• Bhattacharya, S., Sil, P. C., 2018. Role of plant-derived polyphenols in reducing oxidative stress-mediated diabetic complications. Reactive oxygen species, 5(13), 15-34.
• Bindhumol, V., Chitra, K. C., Mathur P. P., 2003. Bisphenol A inducers reactive oxygen species generation in the liver of male rats. Toxicology, 188(2-3), 117-124.
• Del Castillo, M. D., Ames, J. M., Gordon, M. H., 2002. Effect of roasting on the antioxidant activity of coffee brews. Journal of Agricultural and Food Chemistry, 50(13), 3698-3703.
• Dewanto, V., Wu, X., Liu, R. H., 2002. Processed sweet corn has higher antioxidant activity. Journal of Agricultural and Food Chemistry, 50(17), 4959-4964.
• Ebert, E. C., Nagart, M., 2008. Gastrointestinal manifestations of amyloidosis. The American Journal of Gastroenterology, 103(3), 776-787.
• Edres, H. A., Taha, N. M., Mandour, A. E. W. A., Lebda, M. A., 2018. Impact of L-carnitine on bisphenol A-induced kidney damage in rats. Alexandria Journal of Veterinary Sciences, 56(1), 11-17.
• Eshak, M. G., Osman, H. F., 2014. Biological effects of chitosan against bisphenol-A induced endocrine toxicity and androgen receptor gene expression changes in male rats. International Journal of Pharmaceutical and Clinical Research, 6(4), 300-311.
• Fisher, J. W., Twaddle, N. C., Vanlandingham, M., Doerge, D. R., 2011. Pharmacokinetic modeling: prediction and evaluation of route dependent dosimetry of bisphenol A in monkeys with extrapolation to humans. Toxicology and Applied Pharmacology, 257(1), 122-136.
• Godos, J., Pluchinotta, F. R., Marventano, S., Buscemi, S., Volti, G. L., Galvano, F., Grosso, G., 2014. Coffee components and cardiovascular risk: beneficial and detrimental effects. International Journal of Food Sciences and Nutrition, 65(8), 925-936.
• Gülçin, İ., 2010. Antioxidant properties of resveratrol: a structure–activity insight. Innovative Food Science and Emerging Technologies, 11(1), 210-218.
• Haile, M., Kang, W. H., 2019. Antioxidant activity, total polyphenol, flavonoid and tannin contents of fermented green coffee beans with selected yeasts. Fermentation, 5(1), 29.
• Hassan, Z. K., Elobeid, M. A., Virk, P., Omer, S. A., El Amin, M., Daghestani, M. H., Al Olayan, E. M., 2012. Bisphenol a induces hepatotoxicity through oxidative stress in rat model. Oxidative Medicine and Cellular Longevity, 2012, 1-6.
• Haroun, M. R., Zamzam, I. S., Metwally, E. S., El-Shafey, R. S., 2019. Effect of vitamin C on bisphenol A induced hepato and nephrotoxicity in albino rats. The Egyptian Journal of Forensic Sciences and Applied Toxicology, 16, 57-85.
• Hoque, E., Sujan, K. M., Haque, M. I., Mustary, A., Miah, M. A., Hossain, M. M., Islam, M. K., 2019. Evaluation of bisphenol A induced effects on blood biochemical constituents and histo-structure of liver in Swiss albino mice and its ‘one health’perspectives. Journal of Veterinary Medical and One Health Research, 1(1), 75-85.
• Hosseinabadi, S., Rafraf, M., Asghari, S., Asghari-Jafarabadi, M., Vojouhi, S., 2020. Effect of green coffee extract supplementation on serum adiponectin concentration and lipid profile in patients with non-alcoholic fatty liver disease: a randomized, controlled trial. Complementary Therapies in Medicine, 49, 1-7.
• İştar, B., Yapar, K., Acar, A., Yalçın, E., Seven, B., Çavuşoğlu, K., 2016. Investigation of protective role of green coffee against genotoxicity induced by bisphenol A in albino mice. Cumhuriyet Science Journal, 37(4), 339-351.
• Jamali, N., Mostafavi-Pour, Z., Zal, F., Kasraeian, M., Poordast, T., Ramezani, F., Zare, R., 2019. Combination effect of caffeine and caffeic acid treatment on the oxidant status of ectopic endometrial cells separated from patients with endometriosis. Iranian Journal of Medical Sciences, 44(4), 315.
• Kose, O., Rachidi, W., Beal, D., Erkekoglu, P., Fayyad‐Kazan, H., Kocer Gumusel, B., 2020. The effects of different bisphenol derivatives on oxidative stress, DNA damage and DNA repair in RWPE‐1 cells: A comparative study. Journal of Applied Toxicology, 40(5), 643-654.
• Liu, Q. M., Yang, X. M., Zhang, L., Majetich, G., 2010. Optimization of ultrasonic-assisted extraction of chlorogenic acid from Folium eucommiae and evaluation of its antioxidant activity. Journal of Medicinal Plants Research, 4(23), 2503-2511.
• Mehari, B., Chandravanshi, B. S., Redi-Abshiro, M., Combrinck, S., McCrindle, R., Atlabachew, M., 2021. Polyphenol contents of green coffee beans from different regions of Ethiopia. International Journal of Food Properties, 24(1), 17-27.
• Melzer, D., Osborne, N. J., Henley, W. E., Cipelli, R., Young, A., Money, C., Mc Cormack, P., Luben, R., Khaw, K. T., Wareham, N. J., Galloway, T. S. 2012. Urinary bisphenol a concentration and risk of future coronary artery disease in apparently healthy men and women. Circulation, 125(12), 1482-1490.
• Nadanasabapathi, S., Rufia, J., ManjuV. V., 2013. In vitro free radical scavenging activity and bioavailability of dietary compounds caffeine, caffeic acid and their combination. International Food Research Journal, 20(6), 3159.
• Naidu, M. M., Sulochanamma, G., Sampathu, S. R., Srinivas, P., 2008. Studies on extraction and antioxidant potential of green coffee. Food Chemistry, 107(1), 377-384.
• Navarra, G., Moschetti, M., Guarrasi, V., Mangione, M. R., Militello, V., Leone, M., 2017. Simultaneous determination of caffeine and chlorogenic acids in green coffee by UV/Vis spectroscopy. Journal of Chemistry, 2017, 1-8.
• Nogaim, Q. A., Bugata, L. S. P., Prabhakar, P. V., Reddy, U. A., Kumari, I., Mahboob, M., 2020. Protective effect of Yemeni green coffee powder against the oxidative stress induced by Ochratoxin A. Toxicology Reports, 7, 142-148.
• Poormoosavi, S. M., Najafzadehvarzi, H., Behmanesh, M. A., Amirgholami, R., 2018. Protective effects of Asparagus officinalis extract against bisphenol A-induced toxicity in Wistar rats. Toxicology Reports, 5, 427-433.
• Salamat, S., Sharif, S. S., Nazary-Vanani, A., Kord-Varkaneh, H., Clark, C. C., Mohammadshahi, M., 2019. The effect of green coffee extract supplementation on serum oxidized LDL cholesterol and total antioxidant capacity in patients with dyslipidemia: A randomized, double-blind, placebo-controlled trial. The European Journal of Integrative Medicine, 28, 109-113.
• Santovito, A., Cannarsa, E., Schleicherova, D., Cervella, P., 2018. Clastogenic effects of bisphenol A on human cultured lymphocytes. Human and Experimental Toxicology, 37(1), 69-77.
• Schott, D. H., Collins, R. N. Bretscher, A., 2002. Secretory vesicle transport velocity in living cells depends on the myosin V lever arm length. Journal of Cell Biology, 156(1), 35-39.
• Shahmohammadi, H. A., Hosseini, S. A., Hajiani, E., Malehi, A. S., Alipour, M., 2017. Effects of green coffee bean extract supplementation on patients with non-alcoholic fatty liver disease: a randomized clinical trial. Hepatitis Monthly, 17(4), 1-9.
• Singleton, V. L., Orthofer, R., Lamuela-Raventós, R. M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152–178.
• Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Sasaki, Y. F., 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis, 35(3), 206-221.
• Vinson, J. A., Chen, X., Garver, D. D., 2019. Determination of total chlorogenic acids in commercial green coffee extracts. Journal of Medicinal Food, 22(3), 314-320.
• Yalçın, E., Çavuşoğlu, K., Acar, A., Yapar, K., 2020. In vivo protective effects of Ginkgo biloba L. leaf extract against hydrogen peroxide toxicity: cytogenetic and biochemical evaluation. Environmental Science and Pollution Research, 27(3), 3156-3164.
• Yalçın, E., Uzun, A., Çavuşoğlu, K., 2019. In vivo epiclorohidrine toxicity: cytogenetic, biochemical, physiological and anatomical evidences. Environmental Science and Pollution Research, 26, 22400-22406.
• Yuan, J., Kong, Y., Ommati, M. M., Tang, Z., Li, H., Li, L., Zhao, C., Shi, Z., Wang, J., 2019. Bisphenol A-induced apoptosis, oxidative stress and DNA damage in cultured rhesus monkey embryo renal epithelial Marc-145 cells. Chemosphere, 234, 682-689.
• Yukawa, G., Mune, M., Otani, H., Tone, Y., Liang, X. M., Iwahashi, H., Sakamoto, W., 2004. Effects of coffee consumption on oxidative susceptibility of low-density lipoproteins and serum lipid levels in humans. Biochemistry (Moscow), 69(1), 70-74.