Insecticidal and Antimicrobial Effects of Pseudomonas Species Isolated From Waste Water

Year 2022, Volume: 11 Issue: 3, 233 - 240, 31.12.2022

Baran Seven

Abstract

In this study, the insecticidal effects of Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida isolated from refinery wastewater on Leptinotarsa decemlineata and Dendroctonus micans larvae and antimicrobial effects against different microorganisms were investigated. L. decemlineata and D. micans larvae collected from the Kümbet Plateau of Giresun province were fed with isolate inoculated leaf and bark samples. At the end of 7 days of application, the viability and mortality rates were calculated. Antimicrobial activities of isolates against gram negative, gram positive and fungal species were investigated by disc diffusion method using fermentation broths. As result, at the end of the 7-day application, a mortality rate of 69-85% was obtained for L. decemlineata larvae. On the other hand, 49-78% mortality was obtained in D.micans larvae. This finding indicates that D. micans is more sensitive to isolates. It was determined that the isolates exhibited different levels of antimicrobial activity against gram negative, gram positive and Candida species. These results show that Pseudomonas species isolated from refinery wastewater have high potential in biological control against L. decemlineata and D. micans and exhibit broad-spectrum antimicrobial activity.

Keywords

Biological control, Dendroctonus micans, Leptinotarsa decemlineata, Pseudomonas sp.

References

• Alkan A., H. A. Z. A. N., Eroğlu, M., Özcan, G. 2014. Attack Strategy and Development af Dendroctonus micans (Kug.)(Coleoptera: Curculionidae) On Oriental Spruce İn Turkey. Turkıye Entomolojı Dergısı-Turkısh Journal Of Entomology. 38(1).
• Amankwah, F. K. D., Gbedema, S. Y., Boakye, Y. D., Bayor, M. T., Boamah, V. E. 2022. Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate. Scientifica, 2022.
• Bale, J. S., van Lenteren, J. C., Bigler, F. 2008. Biological Control and Sustainable Food Production. Philosophical Transactions of the Royal Society of London ,. 363, 761–776.
• Bekci, H., Yuvali Celik, G., Onbasili, D. 2018. Antimicrobial activities of Pseudomonas spp. strains isolated from raw milk collected in Turkey. International Journal of Research Science Management. 6(3), 80-88.
• Bensidhoum, L., Nabti, E., Tabli, N., Kupferschmied, P., Weiss, A., Rothballer, M., et al. 2016. Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities. European Journal of Soil Biology. 75, 38-46.
• Chen, W. J., Hsieh, F. C., Hsu, F. C., Tasy, Y. F., Liu, J. R., Shih, M. C. 2014. Characterization of an Insecticidal Toxin and Pathogenicity of Pseudomonas taiwanensis against insects. PLoS Pathogens. 10(8), e1004288.
• Dahmana, H., Raoult, D., Fenollar, F., Mediannikov, O. 2020. Insecticidal Activity Of Bacteria From Larvae Breeding Site With Natural Larvae Mortality: Screening of Separated Supernatant and Pellet Fractions. Pathogens, 9(6), 486.
• Dhanasekaran, D., Thangaraj, R. 2014. Microbial secondary metabolites are an alternative approaches against insect vector to prevent zoonotic diseases. Asian Pacific Journal of Tropical Disease. 4, 253-261.
• Durhan, B., Yalçın, E., Çavuşoğlu, K., Acar, A. 2022. Molecular Docking Assisted Biological Functions And Phytochemical Screening of Amaranthus lividus L. Extract. Scientific Reports, 12(1), 1-16.
• Li, H., Medina, F., Vinson, S.B., Coates C.J. 2005. Isolation, Characterization, and Molecular Identification Of Bacteria From The Red İmported Fire Ant (Solenopsis invicta) midgut. Journal of Invertebrate Pathology. 89, 203-209.
• Lim, H. S., Kim, Y. S., Kim, S. D. 1991. Pseudomonas stutzeri YPL-1 Genetic Transformation And Antifungal Mechanism Against Fusarium solani, An Agent Of Plant Root Rot. Applied and Environmental Microbiology. 57(2), 510-516.
• Motta, A. S., Cladera-Olivera, F., Brandelli, A. 2004. Screening for antimicrobial activity among bacteria isolated from the Amazon basin. Brazilian Journal of Microbiology. 35, 307-310.
• Schneider, M., Dorn, A. 2001. Differential Infectivity Of Two Pseudomonas species And The Immune Response İn The Milkweed Bug, Oncopeltus fasciatus (Insecta: Hemiptera). Journal of Invertebrate Pathology. 78(3), 135-140.
• Singh, H., Kaur, M., Jangra, M., Mishra, S., Nandanwar, H., Pinnaka, A. K. 2019. Antimicrobial properties of the novel bacterial isolate Paenibacilllus sp. SMB1 from a halo-alkaline lake in India. Scientific Reports. 9, 1-12.
• Sokolov, V.E. 1981. The Colorado beetle, Leptinotarsa decemlineata Say. Phylogeny, morphology, physiology, ecology, adaptation, natural enemies, Nauka, Moscow, (RU), 375.
• Timmis, K.N. 2002. Pseudomonas putida: A Cosmopolitan Opportunist Par Excellence. Environmental Microbiology. 4, 779-781.
• Tronsmo, A., Harman, G. E. 1993. Detection And Quantification of N-acetyl-β-D-Glucosaminidase, Chitobiosidase, And Endochitinase In Solutions And On Gels. Analytical Biochemistry, 208(1), 74-79.
• Yalçın, E., Ergene, A. 2009. Screening The Antimicrobial Activity Of Biosurfactants Produced By Microorganisms İsolated From Refinery Wastewaters. Journal of Applied Biological Sciences. 3(2), 163-168.
• Vesga, P., Augustiny, E., Keel, C., Maurhofer, M., & Vacheron, J. 2021. Phylogenetically closely related pseudomonads isolated from arthropods exhibit differential insect‐killing abilities and genetic variations in insecticidal factors. Environmental Microbiology. 23, 5378-5394.
• Wang, S. L., Chang, W. T. 1997. Purification And Characterization Of Two Bifunctional Chitinases/Lysozymes Extracellularly Produced By Pseudomonas aeruginosa K-187 in a Shrimp And Crab Shell Powder Medium. Applied and Environmental Microbiology. 63(2), 380-386.