17-α-Metiltestosteron Hormonu Kullanımının Lepistes (Poecilia reticulata) Balıklarında Renklenme, Cinsiyet Dönüşümü ve Üreme Performansı Üzerine Etkisi

Year 2023, Volume: 12 Issue: 1, 55 - 66, 30.06.2023

Ebru Yılmaz , Muammer Erdem

An Erratum to this article was published on November 17, 2023. https://dergipark.org.tr/en/pub/gbad/issue/80054/1372256

Abstract

Bu araştırmada, yeme ve suya 17 -metiltestosteron hormonu katılarak lepistes balıklarında (Poecilia reticulata) renklenme, cinsiyet dönüşümü ve üreme üzerine etkileri araştırılmıştır. Deneme grupları; kontrol grubu (K), suya hormon (S) ve yeme hormon (Y) grupları olarak 3 grupta 3 tekerrürlü olacak şekilde planlanmıştır. 3 aşamalı olan çalışma toplam 12 hafta sürmüştür. Denemenin 1. ve 2. aşamalarında haftalık periyotlarda renk skalası kullanılarak 10 panelist ile görsel renk tayini yapılmıştır. 1 ml’sinde 0.1 mg MT bulunan stok çözeltiden suya ve yeme katılmış, kontrol grubuna ise hormon uygulanmamıştır. Denemenin 1. aşaması sonunda renk skalası değerleri ortalaması en yüksek S grubunda (29.90±0.23) tespit edilmiş olup, bunu Y (29.10±0.18) ve K (20±0.00) grupları takip etmiş, gruplar arasında istatistiksel farkın önemli olduğu belirlenmiştir (p<0.05). Denemenin 2. aşaması sonunda renk skalası değerleri ortalaması en yüksek S grubunda (30.83±0.13) tespit edilmiş olup, bunu Y (29.60±0.11) ve K (20±0.00) grupları takip etmiş, gruplar arasında fark istatistiksel açıdan önemli bulunmuştur (p<0.05). Denemede S grubunda 1. aşamada 5 adet, 2. aşamada 1 adet toplam 6 adet balıkta, Y grubunda ise 1. ve 2. aşamalarda 1’er adet, toplam 2 adet balıkta gonopodium oluşumu gözlenmiştir. S grubunda androjen hormonun daha fazla cinsiyet dönüşümüne neden olduğu görülmüştür. Üremeye etkisi bakımından çalışma sonucuna baktığımızda S ve Y gruplarında üreme olmazken K grubunda 2 balıktan toplam 18 yavru elde edilmiştir. Sonuç olarak 17α-MT hormon kullanımının lepistes balıklarında renklenme ve cinsiyet dönüşümü üzerinde etkisinin olumlu olduğu, üreme üzerine etkisinin ise olumsuz olduğu tespit edilmiştir.

Keywords

Cinsiyet Dönüşümü, Lepistes, Poecilia reticulata, Renklenme, Üreme, 17-α-metiltestosteron, Cinsiyet Dönüşümü, Lepistes, Poecilia reticulata, Renklenme, Üreme, 17-α-metiltestosteron

Effects of 17-α-Methyltestosterone usage on Coloration, Sex Reversal and Reproductive Performance in Guppy (Poecilia reticulata) Fish

An Erratum to this article was published on November 17, 2023. https://dergipark.org.tr/en/pub/gbad/issue/80054/1372256

Abstract

In this study, 17 -methyltestosterone hormone was added to the feed and water, and the effects of coloring, sex reversal and reproduction were investigated in guppy fish (Poecilia reticulata). Experimental groups were planned as control group (K), water hormone (S) and feeding hormone (Y) groups in 3 groups with 3 replicates. The 3 -stage study lasted a total of 12 weeks. In the 1st and 2nd stages of the experiment, visual color determination was performed with 10 panelists using a color scale at weekly intervals. 1 ml of the stock solution containing 0.1 mg MT was added to water and feed, while the control group was not treated with hormone. At the end of the 1st phase of the experiment, the highest mean color scale values were found in the S group (29.90±0.23), followed by the Y (29.10±0.18) and K (20±0.00) groups, and significant differences were determined between the groups (p<0.05). At the end of the 2nd phase of the experiment, the highest mean color scale values were found in the S group (30.83±0.13), followed by the Y (29.60±0.11) and K (20±0.00) groups, and the difference between the groups was found statistically significant (p<0.05). In the experiment, gonopodium formation was observed in S group in the 1st stage in 5 fish and in the 2nd stage in 1 fish in total 6 fish, and in Y group in the 1st and 2nd stages in 1 fish in total 2 fish. In group S, androgen hormone was found to cause more sex reversal. The results of the current study in terms of the effect of hormone on reproduction, while there was no reproduction in the S and Y groups, a total of 18 fry were obtained from 2 fish in the K group. As a result, it was determined that the effect of 17α-MT hormone use on coloration and sex reversal in guppies was positive, while its effect on reproduction was negative.

Keywords

Sex reversal, Guppy, Poecilia reticulata, Coloration, Reproduction, 17α-methyltestosterone

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