Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması

Derya Koyuncu Zeybek; Bülent Zeybek; Nuran Özçiçek Pekmez; Esma Kılıç; Şule Pekyardımcı

EN TR

Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması

Abstract

Bu çalışmada, fenolik bileşiklerin tayini için polimer film temelli amperometrik enzim elektrotlar hazırlandı. Bu amaçla, o-fenilendiamin (o-PD) veya o-PD ve 3-metiltiyofen (3-MT) içeren asetonitril-su karışımı kullanılarak platin elektrotlar elektropolimerizasyon yoluyla modifiye edildi. Hazırlanan Pt/PoPD ve Pt/PoPD-3MT elektrotlara polifenol oksidaz (POx) enzimi immobilize edildi ve elektrotların pirokateşole duyarlıkları belirlendi. Daha yüksek duyarlığın elde edildiği Pt/PoPD-3MT/POx elektrot ile çalışma koşulları ve performans faktörleri çalışıldı. Pt/PoPD-3MT/POx elektrot için optimum tampon cinsi, tampon derişimi, pH\'sı, sıcaklık ve çalışma potansiyeli sırasıyla fosfat; 0,10 M; 7,0; 25°C ve Ag/AgCl\'e karşı -0,20 V olarak belirlendi. Enzim elektrodun doğrusal çalışma aralığı 1,96×10–6- 3,50×10-5M olarak belirlendi. Enzim

Keywords

Amperometri, Enzim elektrot, Fenolik bileşikler, Polifenol oksidaz, Poli ofenilendiamin, 3 Metiltiyofen.

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Details

Primary Language

Turkish

Subjects

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Journal Section

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Authors

Derya Koyuncu Zeybek This is me

Bülent Zeybek This is me

Nuran Özçiçek Pekmez This is me

Esma Kılıç This is me

Şule Pekyardımcı This is me

Publication Date

September 1, 2010

Submission Date

November 21, 2014

Acceptance Date

-

Published in Issue

Year 2010 Volume: 30 Number: 3

IZ

https://izlik.org/JA24SM43FG

APA

Zeybek, D. K., Zeybek, B., Pekmez, N. Ö., Kılıç, E., & Pekyardımcı, Ş. (2010). Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması. Gazi Eğitim Fakültesi Dergisi, 30(3), 955-978. https://izlik.org/JA24SM43FG

AMA

1.Zeybek DK, Zeybek B, Pekmez NÖ, Kılıç E, Pekyardımcı Ş. Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması. GUJGEF. 2010;30(3):955-978. https://izlik.org/JA24SM43FG

Chicago

Zeybek, Derya Koyuncu, Bülent Zeybek, Nuran Özçiçek Pekmez, Esma Kılıç, and Şule Pekyardımcı. 2010. “Fenolik Bileşiklerin Tayini Için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması”. Gazi Eğitim Fakültesi Dergisi 30 (3): 955-78. https://izlik.org/JA24SM43FG.

EndNote

Zeybek DK, Zeybek B, Pekmez NÖ, Kılıç E, Pekyardımcı Ş (September 1, 2010) Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması. Gazi Eğitim Fakültesi Dergisi 30 3 955–978.

IEEE

[1]D. K. Zeybek, B. Zeybek, N. Ö. Pekmez, E. Kılıç, and Ş. Pekyardımcı, “Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması”, GUJGEF, vol. 30, no. 3, pp. 955–978, Sept. 2010, [Online]. Available: https://izlik.org/JA24SM43FG

ISNAD

Zeybek, Derya Koyuncu - Zeybek, Bülent - Pekmez, Nuran Özçiçek - Kılıç, Esma - Pekyardımcı, Şule. “Fenolik Bileşiklerin Tayini Için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması”. Gazi Eğitim Fakültesi Dergisi 30/3 (September 1, 2010): 955-978. https://izlik.org/JA24SM43FG.

JAMA

1.Zeybek DK, Zeybek B, Pekmez NÖ, Kılıç E, Pekyardımcı Ş. Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması. GUJGEF. 2010;30:955–978.

MLA

Zeybek, Derya Koyuncu, et al. “Fenolik Bileşiklerin Tayini Için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması”. Gazi Eğitim Fakültesi Dergisi, vol. 30, no. 3, Sept. 2010, pp. 955-78, https://izlik.org/JA24SM43FG.

Vancouver

1.Derya Koyuncu Zeybek, Bülent Zeybek, Nuran Özçiçek Pekmez, Esma Kılıç, Şule Pekyardımcı. Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması. GUJGEF [Internet]. 2010 Sep. 1;30(3):955-78. Available from: https://izlik.org/JA24SM43FG

Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması

Preparation of Amperometric Enzyme Electrode Based on Polyphenol Oxidase for Determination of Phenolic Compounds

Abstract

Keywords

Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması

Abstract

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