Çölyak Hastalığı ve Plazminojen Aktivatör İnhibitör-1 Polimorfizminin İlişkisi

Year 2022, , 601 - 604, 29.10.2022

Deniz Ağırbaşlı , Minenur Kalyoncu , Nur Ramoğlu , Zuhal Çalışkan

https://doi.org/10.54005/geneltip.1190070

Abstract

Amaç: Çölyak hastalığı (ÇH) otoimmün bir hastalıktır. Çölyak hastalarında tromboza yatkınlık vaka çalışmalarında bildirilmiş olmasına rağmen nedeni henüz bilinmemektedir. Plazminojen aktivatör inhibitörü-1 (PAI-1), fibrinolizi inhibe eder. Bu çalışmada biyopsi ile çölyak tanısı konulmuş kişiler ve sağlıklı kontrollerde çölyak hastalığı ile PAI-1 4G/5G polimorfizmi arasındaki ilişki araştırılmıştır.
Yöntemler: Çalışma için 56 biyopsi ile tanı konulmuş çölyak hastası ve 52 sağlıklı kontrol seçilmiştir. PAI-1 genotiplendirmesi PZR-RFLP metodu ile yapılmış, sonuçlar istatistiksel anlamlılığa göre karşılaştırılmıştır.
Bulgular: Trombotik risk ve enflamasyonla ilişkilendirilen PAI-1 4G aleli vakalarda 5G aleline oranla yüksek bulundu (sırasıyla 0.63 ve 0.36, p=0.054). PAI-1 varyantlarına göre tanı yaşları kıyaslandığında anlamlı bir fark bulunmadı (p=0.3). Genotip olarak 4G/4G hastalar ve kontrollerde benzer olsa da koruyucu genotip olarak bilinen 5G/5G genotipi kontrollerde hastalara kıyasla yüksek bulunmuştur (35% ve 18%).
Sonuç: Çalışmamızda çölyak hastalarında kontrol guruba göre PAI-1’in tromboza yatkınlıkta rol oynayan 4G aleli daha sık gözlenmiştir. Çölyak hastalarında tromboz genetik belirteçlerinin çalışılması bireysel proflaksi açısından önem taşımaktadır.

Keywords

Çölyak hastalığı, Plazminojen aktivatör inhibitörü 1, Polimorfizm, Tromboz

Supporting Institution

Acıbadem Mehmet Ali Aydınlar University

Project Number

ABAPKO Decision Number :2017/01/06.

Association of Celiac Disease and Plasminogen Activator Inhibitor-1 Polymorphism

Abstract

Objective: Celiac disease (CD) is an autoimmune disease. Although susceptibility to thrombosis in celiac patients has been reported in case studies, the cause is not yet known. PAI-1 inhibits fibrinolysis. In this study, the association between celiac disease and PAI-1 4G/5G polymorphism was investigated among celiac patients whose disease were confirmed by intestinal biopsy and healthy controls.
Methods: Biopsy-diagnosed celiac patients (n=56) and healthy controls (n=52) were included in the study. PCR-RFLP method was used for PAI-1 genotyping and the results were compared according to statistical significance.
Results: The PAI-1 4G allele associated with thrombotic risk and inflammation was found to be higher than the 5G allele in cases (0.63 and 0.36, respectively, p=0.054). When the age at diagnosis was compared according to the PAI-1 variants, no significant difference was found (p=0.3). Although the genotype frequencies were similar in 4G/4G patients and controls, the 5G/5G genotype, known as the protective genotype, was found to be higher in controls (35% and 18%).
Conclusion: In our study, the 4G allele of PAI-1, which plays a role in the susceptibility to thrombosis, was observed more frequently in celiac patients compared to the control group. Studying genetic markers of thrombosis in celiac patients is important for individual prophylaxis.

Keywords

Celiac disease, Plasminogen Activator Inhibitor 1, Polymorphizm, Thrombosis

Project Number

ABAPKO Decision Number :2017/01/06.

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