Gestasyonel Diyabetes Mellituslu Gebe Hastalarda Maternal ve Umblikal Kordon Heat-Shock Protein 70 Seviyeleri

Year 2024, , 251 - 257, 30.04.2024

Mete Bertizlioğlu , Setenay Yılmaz , Ayşegül Kebapçılar , Aybike Tazegul , Özlem Seçilmiş , Berat Berrin Gençoğlu Bakbak , Sedat Abuşoğlu , Cetin Celik , Ali Unlu , Nasuh Utku Doğan , Görkem Aktaş

https://doi.org/10.54005/geneltip.1400341

Abstract

AMAÇ: Bu çalışmanın amacı, hücresel stresin bir belirleyicisi olan Heat shock protein 70 (Hsp-70)'in, gestasyonel diyabet mellitus (GDM) gebeliklerde maternal serum ve umbilikal kordda yüksek olup olmadığını belirlemek ve farklı serum Hsp-70 konsantrasyonlarının umbilikal kordda, GDM'li kadınlarda ve kontrol grubundaki kadınlarda erken doğumun bir göstergesi olarak ilişkilendirilip ilişkilendirilmediğini belirlemektir (37 0/7-38 6/7 hafta gebelik).
YÖNTEMLER: Çalışma, bu durum kontrol çalışmasında sezaryen doğum olan GDM'li 62 hasta (GDM grubu) ve 22 diyabetik olmayan, sağlıklı kadını içermektedir. Hamileliklerde ve umbilikal kord serumlarında Hsp-70 düzeylerini ve tüm konulardaki erken doğumu içeren diğer biyokimyasal ve antropometrik belirteçleri analiz ettik.
BULGULAR: GDM'li hastalarda maternal serum Hsp-70 düzeyleri, sağlıklı hamile kadınlardan önemli ölçüde yüksekti. GDM hastalarının umbilikal kord Hsp-70 düzeyleri de sağlıklı hamile kadınlara göre artmıştı ancak genellikle kabul edilebilir düzeyde anlam bulunamadı. Kord Hsp-70 düzeyleri, GDM'li kadınlarda doğum zamanı ile negatif anlamlı bir korelasyon gösterdi. Kord Hsp-70 düzeyleri, aynı zamanda tüm grup içinde doğum zamanı ile negatif anlamlı bir korelasyon gösterdi.
SONUÇLAR: Maternal Hsp-70, GDM'li hastalarda önemli ölçüde yüksekti. Elde edilen sonuçlar, yüksek umbilikal kord Hsp-70 değerlerinin, gebeliklerde erken doğum için bir risk faktörü göstergesi olarak potansiyel olarak kullanılabileceğini göstermektedir.

Keywords

heat-shock protein 70, gestasyonel diyabetes mellitus, erken doğum

Maternal and Umbilical Cord Heat-Shock Protein 70 Levels in Patients with Gestational Diabetes Mellitus

Abstract

AIMS: The purpose of this study is to determine if heat shock protein 70 (Hsp-70), a marker of cellular stress, is elevated in maternal serum and umbilical cord in pregnancies complicated by gestational diabetes mellitus (GDM) and to determine whether altered serum Hsp-70 concentrations in umbilical cord are related to serve as an indicator of early term delivery (37 0/7–38 6/7 weeks of gestation) in women with GDM and control group.
METHODS: The study included 62 patients with GDM (GDM group) and 22 non-diabetic, healthy women before caesarean section in this case-control study. We analyzed serum levels of Hsp-70 in pregnancies and umbilical cord sera and other biochemical and anthropometric markers, early term delivery in all subjects.
RESULTS: Maternal Serum levels of Hsp-70 were significantly higher in patients with GDM than in healthy pregnant women. The umbilical cord levels of Hsp-70 in GDM patients were also increased as compared to healthy pregnant women but missed the commonly acceptable significance level. Cord Hsp-70 levels showed a negatively significant correlation with time of delivery, in women with GDM patients. Cord Hsp-70 levels showed a negatively significant correlation with time of delivery, also in the whole group.
CONCLUSIONS: Maternal Hsp-70 was significantly higher in patients with GDM. The obtained results seem to indicate that elevated umbilical cord Hsp-70 values may potentially be used as indicators of risk factor for early term delivery in pregnancies.

Keywords

gestational diabetes mellitus, heat-shock protein 70, Preterm Birth

References

• Hanna F, Peters J, Harlow J, Jones PJQAIJoM. Gestational diabetes screening and glycaemic management; national survey on behalf of the Association of British Clinical Diabetologists. 2008;101(10):777-84.
• Kuhn DC, Crawford MA, Stuart MJ, Botti JJ, Demers LMJD. Alterations in transfer and lipid distribution of arachidonic acid in placentas of diabetic pregnancies. 1990;39(8):914-8.
• Szczechla M, Balewska A, Naskręt D, Zozulińska-Ziółkiewicz D, Uruska AJCIiMB. Molecular Changes in Cells of Patients with Type 2 Diabetes Mellitus Depending on Changes in Glycemia Level in the Context of Lifestyle—An Overview of the Latest Scientific Discoveries. 2023;45(3):1961-81.
• Santoro MGJBp. Heat shock factors and the control of the stress response. 2000;59(1):55-63.
• Nakhjavani M, Morteza A, Khajeali L, Esteghamati A, Khalilzadeh O, Asgarani F, et al. Increased serum HSP70 levels are associated with the duration of diabetes. 2010;15:959-64.
• Köck K, Köck F, Klein K, Bancher-Todesca D, Helmer HJTJoM-F, Medicine N. Diabetes mellitus and the risk of preterm birth with regard to the risk of spontaneous preterm birth. 2010;23(9):1004-8.
• Fukushima A, Kawahara H, Isurugi C, Syoji T, Oyama R, Sugiyama T, et al. Changes in serum levels of heat shock protein 70 in preterm delivery and pre‐eclampsia. 2005;31(1):72-7.
• on the Diagnosis ECJDC. Report of the expert committee on the diagnosis and classification of diabetes mellitus. 2003;26:S5-S20.
• Langer O, Rodriguez DA, Xenakis EM, McFarland MB, Berkus MD, Arredondo FJAjoo, et al. Intensified versus conventional management of gestational diabetes. 1994;170(4):1036-47.
• Langer OJDC. Maternal glycemic criteria for insulin therapy in gestational diabetes mellitus. 1998;21:B91.
• Kavanagh K, Zhang L, Wagner JDJCs, chaperones. Tissue-specific regulation and expression of heat shock proteins in type 2 diabetic monkeys. 2009;14:291-9.
• Wright Jr E, Scism‐Bacon J, Glass LJIjocp. Oxidative stress in type 2 diabetes: the role of fasting and postprandial glycaemia. 2006;60(3):308-14.
• Yabunaka N, Ohtsuka Y, Watanabe I, Noro H, Fujisawa H, Agishi YJDr, et al. Elevated levels of heat-shock protein 70 (HSP70) in the mononuclear cells of patients with non-insulin-dependent diabetes mellitus. 1995;30(2):143-7.
• Gruden G, Bruno G, Chaturvedi N, Burt D, Pinach S, Schalkwijk C, et al. ANTI‐HSP60 and ANTI‐HSP70 antibody levels and micro/macrovascular complications in type 1 diabetes: the EURODIAB Study. 2009;266(6):527-36.
• Molvarec A, Tamási L, Losonczy G, Madách K, Prohászka Z, Rigó JJCS, et al. Circulating heat shock protein 70 (HSPA1A) in normal and pathological pregnancies. 2010;15:237-47.
• Ortega MA, Sánchez-Gil MA, Fraile-Martínez O, García-Montero C, Guijarro LG, Diaz-Pedrero R, et al. Increased Expression of Tetraspanins, ALIX and HSP-70 in the Placenta Tissue of Women with Chronic Venous Disease during Pregnancy. 2023;20(13):1744.
• Kavanagh K, Flynn DM, Jenkins KA, Zhang L, Wagner JDJAJoP-E, Metabolism. Restoring HSP70 deficiencies improves glucose tolerance in diabetic monkeys. 2011;300(5):E894-E901.
• Tan H, Xu Y, Xu J, Wang F, Nie S, Yang M, et al. Association of increased heat shock protein 70 levels in the lymphocyte with high risk of adverse pregnancy outcomes in early pregnancy: a nested case-control study. 2007;12(3):230.
• Shah M, Stanek J, Handwerger SJTHJ. Differential localization of heat shock proteins 90, 70, 60 and 27 in human decidua and placenta during pregnancy. 1998;30:509-18.
• Divers M, Bulmer J, Miller D, Lilford RJG, investigation o. Placental heat shock proteins: no immunohistochemical evidence for a differential stress response in preterm labour. 1995;40(4):236-43.
• Ziegert M, Witkin S, Sziller I, Alexander H, Brylla E, Härtig WJIdio, et al. Heat shock proteins and heat shock protein‐antibody complexes in placental tissues. 1999;7(4):180-5.