Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir

Hülya Uçaryılmaz; Dudu Erkoç Kaya; Hilal Arıkoğlu; Ahmet Bülent Turhan; Mustafa Sait Gönen

doi:10.54005/geneltip.977573

EN TR

SNP-43 polymorphism in CAPN10 gene is associated with Type 2 Diabetes risk in Konya region population

Abstract

Objective: Calpain-10 (CAPN10) is an atypical member of the Ca2+ dependent intracellular cysteine proteases family. It is expressed in many tissues and is essential for multiple cellular functions such as insulin secretion and activity. It is the first type 2 diabetes susceptibility gene identified. In our study, we aimed to analyze the relationship between CAPN10 gene polymorphisms (SNP-44, -43 and -137) and increased type 2 diabetes risk in a Turkish population. Material and Methods: 149 T2D patients and 48 healthy individuals were included in the study. Genotyping was performed using the PCR-SSCP technique, confirmed by DNA sequence analysis. The association between CAPN10 genotypes and T2D development and clinical features was statistically analyzed. Results: According to the results of the odds analysis, while no correlation was observed between SNP-44 and disease (OR: 1.417 CI: 0.452-4.436, P=0.740), a significant correlation was found between SNP-43 and disease (OR: 0.455 CI: 0.235-0.881, P=0.028). In terms of SNP-137 genotype distribution, it was detected as C/C in all patient and control individuals. No correlation was found between the scanned SNPs and clinical parameters (P>0.05). Conclusion: According to our results; SNP-43 in the CAPN10 gene is a risk factor for the development of T2D in the Turkish population.

Keywords

Type 2 Diabetes, Calpain-10, polymorphism

Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir

Abstract

Amaç: Kalpain-10 (CAPN10), Ca+2 bağımlı intrasellüler sistein proteazlar ailesinin atipik bir üyesidir. Birçok dokuda eksprese edilir ve başta insülin sekresyonu ve aktivitesi olmak üzere çok çeşitli hücresel fonksiyonlar için gereklidir. Tanımlanan ilk Tip 2 diyabet (T2D) yatkınlık genidir. Çalışmamızda bir Türk popülasyonunda CAPN10 gen polimorfizmleri (SNP-44, -43 ve -137) ile artmış T2D riski arasındaki ilişkinin analiz edilmesi amaçlandı. Gereç ve Yöntem: Çalışmaya 149 T2D’li hasta ve 48 sağlıklı birey dahil edildi. Genotiplendirme PCR-SSCP tekniği kullanılarak yapıldı, dizi analizi ile doğrulandı. CAPN10 genotiopleri ile T2D gelişimi ve klinik özellikler arasındaki ilişki istatistiksel olarak analiz edildi. Bulgular: Hastalıkla genotip arasındaki ilişkiyi belirlemek için yapılan odds analizi sonuçlarına göre; SNP-44 ile hastalık arasında ilişki gözlenmezken (OR: 1.417 CI:0.452-4.436, P=0.740), SNP-43 ile hastalık arasında anlamlı ilişki bulundu (OR: 0.455 CI:0.235-0.881, P=0.028). SNP-137 genotip dağılımı bakımından tüm hasta ve kontrol bireylerde C/C olarak tespit edildi. Taranan SNP’ler ile klinik parametreler arasında herhangi bir ilişki bulunmadı (P>0.05). Sonuç: Sonuçlarımıza göre; CAPN10 genindeki SNP-43, Türk populasyonunda T2D gelişimi açısından bir risk faktörüdür.

Keywords

Tip 2 Diyabet, Kalpain-10, polimorfizm

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinatörlüğü

Project Number

09202049

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Details

Primary Language

Turkish

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Hülya Uçaryılmaz ^*
0000-0002-0287-5260
Türkiye

Dudu Erkoç Kaya
0000-0003-0114-6602
Türkiye

Hilal Arıkoğlu This is me
0000-0002-6600-6603
Türkiye

Ahmet Bülent Turhan This is me
0000-0002-5972-0147
Türkiye

Mustafa Sait Gönen This is me
Türkiye

Publication Date

September 20, 2021

Submission Date

August 2, 2021

Acceptance Date

August 20, 2021

Published in Issue

Year 2021 Volume: 31 Number: 3

DOI

https://doi.org/10.54005/geneltip.977573

IZ

https://izlik.org/JA87CF86BL

APA

Uçaryılmaz, H., Erkoç Kaya, D., Arıkoğlu, H., Turhan, A. B., & Gönen, M. S. (2021). Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir. Genel Tıp Dergisi, 31(3), 290-294. https://doi.org/10.54005/geneltip.977573

AMA

1.Uçaryılmaz H, Erkoç Kaya D, Arıkoğlu H, Turhan AB, Gönen MS. Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir. Genel Tıp Derg. 2021;31(3):290-294. doi:10.54005/geneltip.977573

Chicago

Uçaryılmaz, Hülya, Dudu Erkoç Kaya, Hilal Arıkoğlu, Ahmet Bülent Turhan, and Mustafa Sait Gönen. 2021. “Konya Bölgesi Popülasyonunda CAPN10 Genindeki SNP-43 Polimorfizmi Tip 2 Diabetes Riski Ile Ilişkilidir”. Genel Tıp Dergisi 31 (3): 290-94. https://doi.org/10.54005/geneltip.977573.

EndNote

Uçaryılmaz H, Erkoç Kaya D, Arıkoğlu H, Turhan AB, Gönen MS (September 1, 2021) Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir. Genel Tıp Dergisi 31 3 290–294.

IEEE

[1]H. Uçaryılmaz, D. Erkoç Kaya, H. Arıkoğlu, A. B. Turhan, and M. S. Gönen, “Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir”, Genel Tıp Derg, vol. 31, no. 3, pp. 290–294, Sept. 2021, doi: 10.54005/geneltip.977573.

ISNAD

Uçaryılmaz, Hülya - Erkoç Kaya, Dudu - Arıkoğlu, Hilal - Turhan, Ahmet Bülent - Gönen, Mustafa Sait. “Konya Bölgesi Popülasyonunda CAPN10 Genindeki SNP-43 Polimorfizmi Tip 2 Diabetes Riski Ile Ilişkilidir”. Genel Tıp Dergisi 31/3 (September 1, 2021): 290-294. https://doi.org/10.54005/geneltip.977573.

JAMA

1.Uçaryılmaz H, Erkoç Kaya D, Arıkoğlu H, Turhan AB, Gönen MS. Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir. Genel Tıp Derg. 2021;31:290–294.

MLA

Uçaryılmaz, Hülya, et al. “Konya Bölgesi Popülasyonunda CAPN10 Genindeki SNP-43 Polimorfizmi Tip 2 Diabetes Riski Ile Ilişkilidir”. Genel Tıp Dergisi, vol. 31, no. 3, Sept. 2021, pp. 290-4, doi:10.54005/geneltip.977573.

Vancouver

1.Hülya Uçaryılmaz, Dudu Erkoç Kaya, Hilal Arıkoğlu, Ahmet Bülent Turhan, Mustafa Sait Gönen. Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir. Genel Tıp Derg. 2021 Sep. 1;31(3):290-4. doi:10.54005/geneltip.977573

The Journal of General Medicine is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY NC).

SNP-43 polymorphism in CAPN10 gene is associated with Type 2 Diabetes risk in Konya region population

Abstract

Keywords

Konya bölgesi popülasyonunda CAPN10 genindeki SNP-43 polimorfizmi Tip 2 Diabetes riski ile ilişkilidir

Abstract

Keywords

Supporting Institution

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