C reaktif protein düzeylerinin farklı cihazlar ve yöntemlerle karşılaştırılması

Year 2020, Volume: 30 Issue: 2, 106 - 111, 01.06.2020

Özgür Aslan Revşa Evin Canpolat Erkan

Abstract

Amaç: C-reaktif protein CRP ; inflamatuvar hastalıklarda, kardiyovasküler olaylarda, travma ve inflamasyonla ilişkili bir çok durumda belirteç olarak kullanılmaktadır. Bu çalışmada farklı cihazlar ile immunnefelometrik ve immunturbidimetrik yöntemler karşılaştırılmıştır.Gereç ve Yöntem: BN II System Siemens Diagnostics, Germany ’de rutin olarak çalışılan 150 CRP numunesi 4 düzey 10 mg/L oluşturularak çalışmaya alındı. Tüm numuneler Immage 800, Architect c16000, Beckman AU680, Cobas C501 cihazlarında çalışıldı.Bulgular: İkili grup olarak immunnefelometrik BN II System ve Immage 800 cihazlarındaki CRP sonuçları karşılaştırıldığında aralarında anlamlı fark olduğu saptanmıştır p

Keywords

Turbidimetre, nefelometre, C-reaktif protein

Comparison of c reactive protein levels with different devices and methods

Abstract

Objective: C-reactive protein CRP is used as a marker in inflammatory diseases, cardiovascular events, and many cases associated with trauma and inflammation. In this study, different devices and immunonephelometric and immunoturbidimetric method were compared.Material and Methods: Routinely studied 150 CRP samples in BN II System Siemens, Germany were included into the study by forming 4 level 10 mg/L . All samples were studied in Immage 800, Architect c16000, Beckman AU680, Cobas C501 devices.Results: When CRP results in BN II System and Immage 800 devices were compared dichotomously, it was found to be a significant difference p

Keywords

Turbidimetry, nephelometry, C-reactive protein

References

• Chan YL, Liao HC, Tsay PK, Chang SS, Chen JC, Liaw SJ. C-reactive protein as an indicator of bacterial infection of adult patients in the emergency department. Chang Gung Med J 2002; 25:437-45.
• Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest 2003;111:1805-12.
• Pinilla JC, Hayes P, Laverty W, Arnold C, Laxdal V. The C-reactive protein to prealbumin ratio correlates with the severity of multiple organ dysfunction. Surgery 1998;124:799-805.
• Pang X, Liu J, Li Y, Zhao J, Zhang X. Emodin Inhibits Ho- mocysteine-Induced C-Reactive Protein Generation in Vascular Smooth Muscle Cell byRegulating PPARγ Exp- ression and ROS-ERK1/2/p38 Signal Pathway. PLoS One 2015;10:e0131295.
• Qu J, L X, Liu Y, Wang X. Evaluation of procalcitonin, C-re- active protein, interleukin-6 & serum amyloid A as diag- nostic biomarkers of bacterial infection in febrile patients. Indian J Med Res 2015;141:315-21.
• Luo Y, Zhang B, Chen M, Jiang T, Zhou D, Huang J, Fu W. Sensitive and rapid quantification of C-reactive prote- in using quantum dot-labeled microplate immunoassay J Transl Med 2012; 10: 24.
• Ledue TB, Weiner DL, Sipe JD, Poulin SE, Collins MF, Rifai N. Analytical evaluation of particle-enhanced immunonep- helometric assays for C-reactive protein, serum amyloid A and mannose-binding protein in human serum. Ann Clin Biochem 1998 ;35:745-53.
• Mali B, Armbruster D, Serediak E, Ottenbreit T. Compa- rison of immunoturbidimetric and immunonephelometric assays for specific proteins. Clin Biochem 2009; 42:1568-71.
• Helal I, Zerelli L, Krid M, ElYounsi F, Maiz BH, Zouari B, et al. Comparison of C-reactive protein and high-sensitivity C-reactive protein levels in patients on hemodialysis. Saudi J Kidney Dis Transpl 2012; 23(3): 477-83.
• Lolekha PH, Chittamma A, Roberts WL, Sritara P, Cheepu- domwit S, Suriyawongpaisal P. Comparative study of two automated high-sensitivity C-reactive protein methods in a large population. Clin Biochem 2005; 38:31-5.
• Marie Dupuy A, Boutet A, Paul Cristol J.Evaluation of the high-sensitivity, full-range Olympus CRP OSR6199 application on the Olympus AU640. Clin Chem Lab Med 2007;45:402-6.
• Correia LC, Lima JC, Gerstenblith G, Magalhães LP, Morei- ra A, Barbosa O Jr, et al. Correlation between turbidimetric and nephelometric methods of measuring C- reactive pro- tein in patients with unstable angina or non-ST elevation acute myocardial infarction. Arq Bras Cardiol 2003; 81:129- 32.
• Wei TQ, Kramer S, Chu VP, Hudson D, Kilgore D, Salyer S, et al. An improved automated immunoassay for C-reactive protein on the Dimension clinical chemistry system. J Au- tom Methods Manag Chem 2000; 22:125-31.
• Ridker PM. C-reactive protein, inflammation, and cardi- ovascular disease: clinical update. Tex Heart Inst J 2005; 32:384-6.
• Ridker PM. A test in context: high-sensitivity C-reactive protein. J Am Coll Cardiol 2016; 67:712–723.
• Monneret D, Mestari F, Djiavoudine S, Bachelot G, Cloi- son M, Imbert-Bismut F, et al. Wide-range CRP versus hi- gh-sensitivity CRP on Roche analyzers: focus on low-grade inflammation ranges and high-sensitivity cardiac troponin T levels. Scand J Clin Lab Invest 2018;78(5):346-51.