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Fruktozdan zengin beslenen sıçanlarda istemli fiziksel aktivitenin metabolik ve kardiyak işlevlere etkisi

Year 2019, Volume: 29 Issue: 2, 65 - 72, 01.03.2019

Pınar Tayfur Orkide Palabıyık Nurşen Uzun Necdet Süt Selma Arzu Vardar

Abstract

Amaç: Fruktozdan zengin beslenme metabolik değişiklikler ve kardiyak hastalık riskini artırıcı etkiler oluşturmaktadır. Bu çalışmanın amacı fruktozdan zengin beslenen sıçanlarda istemli fiziksel aktivitenin metabolik ve kardiyak işlevlerdeki etkilerini araştırmaktır.Gereç ve Yöntem: Erkek Wistar albino sıçanlar kontrol K grubu n=7 , on hafta boyunca fruktozlu içme suyuyla beslenen fruktoz F grubu n=7 ve fruktozlu içme suyuyla beslenerek istemli fiziksel aktivite yapan fruktoz-aktivite FA grubu n=7 olarak ayrıldı. Beslenme sürecinde grupların günlük sıvı alımları ve haftalık vücut ağırlıkları ölçüldü. Beslenme periyodu sonrasında kanda glukoz, trigliserit, total kolesterol, HDL, LDL düzeyleri enzimatik yöntemle, insülin, TNF-α ve IL-6 düzeyleri ELİSA metodu ile belirlendi. Kalpler Langendorff düzeneğine yerleştirilerek sol ventrikül gelişim basıncı, maksimum ve minimum sol ventrikül basınç değişim oranları dp/dt maks ve dp/dt min kaydedildi. Kalp, akciğer ve karaciğer ağırlıkları belirlendi. Bulgular: Kilo alımı FA grupta 95,1±14,3 gr , F grubu 109,0±6,6 gr ve K grubundan 113,4±10,9 gr daha az düzeydeydi p=0,04 ve p=0,03 . Karaciğer ağırlığı F grubunda 11,8±1,0 gr , K 9,7±1,3 gr ve FA 10,2±0,7 gr gruplarından yüksek bulundu p=0,01 ve p=0,01 . Serum glukoz, insülin, trigliserit, total kolesterol, HDL, LDL, TNF-α ve IL-6 düzeylerinde gruplar arasında farklılık bulunmadı. Kardiyak işlev açısından,dp/dt maks düzeyinin FA grupta 2351,6±442,2 , F grubu 1320,7±542,2 ve K grubundan 1756,5±468,7 yüksek olduğubelirlendi p=0,01 ve p=0,05 . Sonuç: Bu çalışma bulguları on hafta boyunca içme suyuna %10 oranında fruktoz eklenerek beslenen sıçanlarda istemli fiziksel aktivitenin kilo alımını azaltıcı ve kardiyak kontraktiliteyi artırıcı rol oynayabileceğini göstermektedir

Keywords

Fruktoz kalp kontraktilite istemli fiziksel aktivite egzersiz

References

  • Mostarda C, Moraes-Silva IC, Salemi VM, et al. Exercise training prevents diastolic dysfunction induced by metabo- lic syndrome in rats. Clinics 2012;67:815-20.
  • Chess DJ, Lei B, Hoit BD, Azimzadeh AM, Stanley WC. De- leterious effects of sugar and protective effects of starch on cardiac remodeling, contractile dysfunction, and mortality in response to pressure overload. Am J Physiol Heart Circ Physiol 2007;293:1853-60.
  • Chess DJ, Xu W, Khairallah R, et al. The antioxidant tempol attenuates pressure overload-induced cardiac hypertrophy and contractile dysfunction in mice fed a high-fructose diet. Am J Physiol Heart Circ Physiol 2008;295:2223-30.
  • Zemancikova A, Torok J. Cardiovascular effects of hi- gh-fructose intake in rats with nitric oxide deficiency. In- terdisciplinary Toxicology 2014;7:159-64.
  • Bidwell AJ, Fairchild TJ, Redmond J, et al. Physical activity offsets the negative effects of a high-fructose diet. Medicine and Science in Sports and Exercise 2014;46:2091-8.
  • Garland T, Jr., Schutz H, Chappell MA, et al. The biologi- cal control of voluntary exercise, spontaneous physical ac- tivity and daily energy expenditure in relation to obesity: human and rodent perspectives. J Experimental Biology 2011;214:206-29.
  • Sherwin CM. Voluntary wheel running: a review and novel interpretation. Animal Behaviour 1998;56:11-27.
  • Garvey SM, Russ DW, Skelding MB, Dugle JE, Edens NK. Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats. Physiological Reports 2015;3:e12319.
  • Samadi Noshahr Z, Shahraki MR, Ahmadvand H, Noura- badi D, Nakhaei A. Protective effects of Withania somnife- ra root on inflammatory markers and insulin resistance in fructose-fed rats. Reports Biochem Mol Biol 2015;3:62-7.
  • Moraes-Silva IC, Mostarda C, Moreira ED, et al. Preventive role of exercise training in autonomic, hemodynamic, and metabolic parameters in rats under high risk of metabolic syndrome development. J Applied Physiol 2013;114:786-91.
  • Lu HR, Whittaker R, Price JH, et al. High Throughput Mea- surement of Ca++ Dynamics in Human Stem Cell-Derived Cardiomyocytes by Kinetic Image Cytometery: A Cardi- ac Risk Assessment Characterization Using a Large Panel of Cardioactive and Inactive Compounds. Toxicological sciences:an official journal of the Society of Toxicology. 2015;148:503-16.
  • Weiss JL, Frederiksen JW, Weisfeldt ML. Hemodynamic de- terminants of the time-course of fall in canine left ventricu- lar pressure. J Clinical Invest 1976;58:751-60.
  • Opie LH. Ventricular function. Heart physiology from cell to circulation. 3th ed. Philadelphia: Lippincott Williams & Wilkins. 1998:343-89.
  • Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Cli- nical Chem 1972;18:499-502.
  • Xu H, Ren X, Lamb GD, Murphy RM. Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats. J Muscle Res Cell Motility 2018;38:1-16
  • Broch-Lips M, de Paoli F, Pedersen TH, Overgaard K, Niel- sen OB. Effects of 8 wk of voluntary unloaded wheel run- ning on K+ tolerance and excitability of soleus muscles in rat. J Applied Physiol 2011;111:212-20.
  • Chang KC, Liang JT, Tseng CD, et al. Aminoguanidine pre- vents fructose-induced deterioration in left ventricular-ar- terial coupling in Wistar rats. Br J Pharmacol 2007;151:341- 6.
  • Lehnen AM, Rodrigues B, Irigoyen MC, De Angelis K, Sc- haan BD. Cardiovascular changes in animal models of me- tabolic syndrome. J Diabetes Res 2013;2013:761314.
  • Mellor KM, Wendt IR, Ritchie RH, Delbridge LM. Fructose diet treatment in mice induces fundamental disturbance of cardiomyocyte Ca2+ handling and myofilament responsi- veness. Am J Physiol Heart Circ Physiol 2012;302:964-72.
  • Bundalo MM, Zivkovic MD, Romic S, et al. Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. J Renin-Angioten- sin-Aldosterone System 2016;17:1470320316642915.
  • Joyeux-Faure M, Rossini E, Ribuot C, Faure P. Fructose-fed rat hearts are protected against ischemia-reperfusion in- jury. Experiment Biol Med 2006;231:456-62.
  • Meirelles CJ, Oliveira LA, Jordao AA, Navarro AM. Meta- bolic effects of the ingestion of different fructose sources in rats. Experimental and clinical endocrinology & diabetes :official journal, German Society of Endocrinology [and] German Diabetes Association 2011;119:218-20.
  • Al-Rasheed N, Al-Rasheed N, Bassiouni Y, Faddah L, Mo- hamad AM. Potential protective effects of Nigella sativa and Allium sativum against fructose-induced metabolic synd- rome in rats. Journal of Oleo Science 2014;63:839-48.
  • de Moura RF, Ribeiro C, de Oliveira JA, Stevanato E, de Mello MA. Metabolic syndrome signs in Wistar rats sub- mitted to different high-fructose ingestion protocols. The Br J Nutr 2009;101:1178-84.
  • Mamikutty N, Thent ZC, Sapri SR, et al. The establishment of metabolic syndrome model by induction of fructose drinking water in male Wistar rats. BioMed research inter- national 2014;2014:263897.
  • Cardinali DP, Bernasconi PA, Reynoso R, Toso CF, Scacchi P. Melatonin may curtail the metabolic syndrome: studies on initial and fully established fructose-induced metabolic syndrome in rats. Int J Mol Sci 2013;14:2502-14.
  • Wilson RD, Islam MS. Fructose-fed streptozotocin-injected rat: an alternative model for type 2 diabetes. Pharmacol Rep 2012;64:129-39.
  • Tappy L, Le KA. Metabolic effects of fructose and the worldwide increase in obesity. Physiological Reviews 2010;90:23-46.
  • Mayes PA. Intermediary metabolism of fructose. Am J Clin Nutr 1993;58:754-65.
  • Meyers A.M., Mourra D, Beeler J.A. High fructose corn syrup induces metabolic dysregulation and altered dopa- mine signaling in the absence of obesity. Plos One 2017 12:e0190206.
  • Kawanishi N, Yano H, Mizokami T, Suzuki K. Mechanisms of chronic inflammation improvement by exercise: Focus on immune response of local tissue. J Physical Fitness and Sports Medicine 2013;2:487-92.

Year 2019, Volume: 29 Issue: 2, 65 - 72, 01.03.2019

Pınar Tayfur Orkide Palabıyık Nurşen Uzun Necdet Süt Selma Arzu Vardar

Abstract

Objective: Fructose-rich diet increases risk of cardiac diseaseand metabolic changes in the body.The aim of this study was to investigate the effect of voluntary physical activity on metabolic and cardiac functionsin fructose-rich feed rats.Material and Methods: Male Wistar albino rats, were divided into groups as control C;n=7 , fructose group which was fed 10% fructose to drinking water F;n=7 and fructose-activity group FA;n=7 housed with a running wheel during 10 weeks. Daily fluid intake and body weight of rats were measured weekly.Serum glucose, triglycerides, total cholesterol, HDL, LDL levels were assessed using an enzymatic method. Insulin, TNF-α , and IL-6 levels were determined by ELISA method. Left ventricular developed pressure, maximum and minimum rate of change were recorded by Langendorff apparatus. The weights of the heart, lungs and liver were determined. Results: Weight gain between the one and the ten weeks were significantly lower for FA group 95,1±14,3 g , in comparison to F 109,0±6,6 g and C 113,4±10,9 g groups p=0,04 and p=0,03 . Liver weight was significantly higher for F group 11,8±1,0 g , in comparison to C 9,7±1,3 g and FA 10,2±0,7 g groups p=0,01 and p=0,01 . Serum measurements were not different between groups. The maximum rate of change was found higher in FA group 2351,6±442,2 than F 1320,7±542,2 and C groups 1756,5±468,7 p=0,01 and p=0,05 . Conclusion: The findings of the study suggest that voluntary physical activity may play a role in reducing weight gain and enhancing cardiac contractility in rats fed with 10% fructose in drinking water for ten weeks

Keywords

Fructose heart voluntary physical activity exercise

References

  • Mostarda C, Moraes-Silva IC, Salemi VM, et al. Exercise training prevents diastolic dysfunction induced by metabo- lic syndrome in rats. Clinics 2012;67:815-20.
  • Chess DJ, Lei B, Hoit BD, Azimzadeh AM, Stanley WC. De- leterious effects of sugar and protective effects of starch on cardiac remodeling, contractile dysfunction, and mortality in response to pressure overload. Am J Physiol Heart Circ Physiol 2007;293:1853-60.
  • Chess DJ, Xu W, Khairallah R, et al. The antioxidant tempol attenuates pressure overload-induced cardiac hypertrophy and contractile dysfunction in mice fed a high-fructose diet. Am J Physiol Heart Circ Physiol 2008;295:2223-30.
  • Zemancikova A, Torok J. Cardiovascular effects of hi- gh-fructose intake in rats with nitric oxide deficiency. In- terdisciplinary Toxicology 2014;7:159-64.
  • Bidwell AJ, Fairchild TJ, Redmond J, et al. Physical activity offsets the negative effects of a high-fructose diet. Medicine and Science in Sports and Exercise 2014;46:2091-8.
  • Garland T, Jr., Schutz H, Chappell MA, et al. The biologi- cal control of voluntary exercise, spontaneous physical ac- tivity and daily energy expenditure in relation to obesity: human and rodent perspectives. J Experimental Biology 2011;214:206-29.
  • Sherwin CM. Voluntary wheel running: a review and novel interpretation. Animal Behaviour 1998;56:11-27.
  • Garvey SM, Russ DW, Skelding MB, Dugle JE, Edens NK. Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats. Physiological Reports 2015;3:e12319.
  • Samadi Noshahr Z, Shahraki MR, Ahmadvand H, Noura- badi D, Nakhaei A. Protective effects of Withania somnife- ra root on inflammatory markers and insulin resistance in fructose-fed rats. Reports Biochem Mol Biol 2015;3:62-7.
  • Moraes-Silva IC, Mostarda C, Moreira ED, et al. Preventive role of exercise training in autonomic, hemodynamic, and metabolic parameters in rats under high risk of metabolic syndrome development. J Applied Physiol 2013;114:786-91.
  • Lu HR, Whittaker R, Price JH, et al. High Throughput Mea- surement of Ca++ Dynamics in Human Stem Cell-Derived Cardiomyocytes by Kinetic Image Cytometery: A Cardi- ac Risk Assessment Characterization Using a Large Panel of Cardioactive and Inactive Compounds. Toxicological sciences:an official journal of the Society of Toxicology. 2015;148:503-16.
  • Weiss JL, Frederiksen JW, Weisfeldt ML. Hemodynamic de- terminants of the time-course of fall in canine left ventricu- lar pressure. J Clinical Invest 1976;58:751-60.
  • Opie LH. Ventricular function. Heart physiology from cell to circulation. 3th ed. Philadelphia: Lippincott Williams & Wilkins. 1998:343-89.
  • Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Cli- nical Chem 1972;18:499-502.
  • Xu H, Ren X, Lamb GD, Murphy RM. Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats. J Muscle Res Cell Motility 2018;38:1-16
  • Broch-Lips M, de Paoli F, Pedersen TH, Overgaard K, Niel- sen OB. Effects of 8 wk of voluntary unloaded wheel run- ning on K+ tolerance and excitability of soleus muscles in rat. J Applied Physiol 2011;111:212-20.
  • Chang KC, Liang JT, Tseng CD, et al. Aminoguanidine pre- vents fructose-induced deterioration in left ventricular-ar- terial coupling in Wistar rats. Br J Pharmacol 2007;151:341- 6.
  • Lehnen AM, Rodrigues B, Irigoyen MC, De Angelis K, Sc- haan BD. Cardiovascular changes in animal models of me- tabolic syndrome. J Diabetes Res 2013;2013:761314.
  • Mellor KM, Wendt IR, Ritchie RH, Delbridge LM. Fructose diet treatment in mice induces fundamental disturbance of cardiomyocyte Ca2+ handling and myofilament responsi- veness. Am J Physiol Heart Circ Physiol 2012;302:964-72.
  • Bundalo MM, Zivkovic MD, Romic S, et al. Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. J Renin-Angioten- sin-Aldosterone System 2016;17:1470320316642915.
  • Joyeux-Faure M, Rossini E, Ribuot C, Faure P. Fructose-fed rat hearts are protected against ischemia-reperfusion in- jury. Experiment Biol Med 2006;231:456-62.
  • Meirelles CJ, Oliveira LA, Jordao AA, Navarro AM. Meta- bolic effects of the ingestion of different fructose sources in rats. Experimental and clinical endocrinology & diabetes :official journal, German Society of Endocrinology [and] German Diabetes Association 2011;119:218-20.
  • Al-Rasheed N, Al-Rasheed N, Bassiouni Y, Faddah L, Mo- hamad AM. Potential protective effects of Nigella sativa and Allium sativum against fructose-induced metabolic synd- rome in rats. Journal of Oleo Science 2014;63:839-48.
  • de Moura RF, Ribeiro C, de Oliveira JA, Stevanato E, de Mello MA. Metabolic syndrome signs in Wistar rats sub- mitted to different high-fructose ingestion protocols. The Br J Nutr 2009;101:1178-84.
  • Mamikutty N, Thent ZC, Sapri SR, et al. The establishment of metabolic syndrome model by induction of fructose drinking water in male Wistar rats. BioMed research inter- national 2014;2014:263897.
  • Cardinali DP, Bernasconi PA, Reynoso R, Toso CF, Scacchi P. Melatonin may curtail the metabolic syndrome: studies on initial and fully established fructose-induced metabolic syndrome in rats. Int J Mol Sci 2013;14:2502-14.
  • Wilson RD, Islam MS. Fructose-fed streptozotocin-injected rat: an alternative model for type 2 diabetes. Pharmacol Rep 2012;64:129-39.
  • Tappy L, Le KA. Metabolic effects of fructose and the worldwide increase in obesity. Physiological Reviews 2010;90:23-46.
  • Mayes PA. Intermediary metabolism of fructose. Am J Clin Nutr 1993;58:754-65.
  • Meyers A.M., Mourra D, Beeler J.A. High fructose corn syrup induces metabolic dysregulation and altered dopa- mine signaling in the absence of obesity. Plos One 2017 12:e0190206.
  • Kawanishi N, Yano H, Mizokami T, Suzuki K. Mechanisms of chronic inflammation improvement by exercise: Focus on immune response of local tissue. J Physical Fitness and Sports Medicine 2013;2:487-92.
There are 31 citations in total.

Details

Primary Language Turkish
Journal Section Original Article
Authors

Pınar Tayfur This is me

Orkide Palabıyık This is me

Nurşen Uzun This is me

Necdet Süt This is me

Selma Arzu Vardar This is me

Publication Date March 1, 2019
Published in Issue Year 2019 Volume: 29 Issue: 2

Cite

Vancouver Tayfur P, Palabıyık O, Uzun N, Süt N, Vardar SA. Fruktozdan zengin beslenen sıçanlarda istemli fiziksel aktivitenin metabolik ve kardiyak işlevlere etkisi. Genel Tıp Derg. 2019;29(2):65-72.

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