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Akut yüzme egzersizi yaptırılan diyabetik sıçanlarda intraperitoneal çinko sülfat uygulamasının kas dokusundaki lipid peroksidasyonuna etkisi

Year 2018, Volume: 28 Issue: 3, 102 - 106, 01.09.2018

Mürsel Biçer Sadettin Ünsal

Abstract

Amaç: Bu çalışmanın amacı, streptozotosin ile diyabet oluşturulmuş akut yüzme egzersizi yaptırılan sıçanlarda çinko uygulamasının kas dokusundaki lipid peroksidasyonu ve antioksidan kapasite üzerindeki etkisinin araştırılmasıdır.Gereç ve Yöntem: Spraque-Dawley cinsi 80 adet erişkin erkek ratlar kullanılan çalışmada. deney hayvanları eşit sayıda 8 gruba ayrıldı: Grup 1, genel kontrol. Grup 2, çinko uygulanan kontrol. Grup 3, çinko uygulanan diyabetli kontrol. Grup 4, yüzme kontrol. Grup 5, çinko uygulanan yüzme. Grup 6, çinko uygulanan diyabetli yüzme. Grup 7, diyabetli yüzme. Grup 8, diyabet grubu. Diyabet oluşturmak için hayvanlara 40 mg/kg dozunda intraperitoneal ip streptozotosin STZ enjekte edildi. Enjeksiyonlar 24 saat sonra aynı dozda tekrarlandı. Son enjeksiyonlardan 6 gün sonra kan glukozu 300 mg/dl ve üzerinde olan hayvanlar diyabetik olarak kabul edildi. Hayvanlara 4 hafta boyunca 6 mg/kg/gün ip çinko sülfat verildi. Dört hafta süren çalışmanın bitiminde deney hayvanlarından dekapitasyonla alınan kas doku örneklerinde MDA nmol/gram/ protein ve GSH mg/dl/gram protein düzeyleri tayin edildi. Bulgular: Kas dokusundaki en yüksek MDA değerleri grup 4 ve 7’de elde edildi. Grup 5 ve 6’nın kas MDA seviyeleri grup 4 ve 7’den düşük, diğer grupların tamamından yüksekti. Grup 5 ve 6 en yüksek kas GSH değerlerine sahipti. Grup 4’ün aynı parametresi grup 5 ve 6’dan düşük diğer grupların tamamından daha yüksekti. Kas dokusundaki en düşük GSH düzeyleri Grup 7 ve 8’de elde edildi.Sonuç: Çalışmanın sonuçları diyabetik sıçanlarda zorlu yüzme egzersizinin yol açtığı kas dokusundaki lipit peroksidasyonu üzerinde intraperitoneal çinko sülfat uygulamasının koruyucu rolü olduğunu göstermektedir

Keywords

Çinko diyabet kas doku lipid peroksidasyon egzersiz

References

  • Baltaci SB, Mogulkoc R, Baltaci AK. Resveratrol and exerci- se. Biomed Rep 2016; 5(5):525-30.
  • Bicer M, Baltaci SB, Patlar S, Mogulkoc R, Baltaci AK. Me- latonin has a protective effect against lipid peroxidation in the bone tissue of diabetic rats subjected to acute swimming exercise. Horm Mol Biol Clin Investig 2018 Mar 16;34(2). pii:/j/hmbci.2018.34.issue-2/hmbci-2017-0079/hmb- ci-2017-0079.xml.
  • Sivrikaya A, Bicer M, Akil M, Baltaci AK, Mogulkoc R. Ef- fects of zinc supplementation on the element distribution in kidney tissue of diabetic rats subjected to acute swim- ming. Biol Trace Elem Res 2012;147(1-3):195-9.
  • Brandao-Neto J, Silva CAB, Rezende AA, Almeida MG, Sa- les VSP, Marchini JS. Zinc pharmacokinetics in insülin–de- pendent diabetes mellitus patients after oral zinc tolerance test. Nutr Res 2003;23:141–50.
  • Gold G, Grodsky GM. Kinetic aspects of comparrmen- tal storage and secretion of insulin and zinc. Experientia 1984;40:1105 –14.
  • Baltaci SB, Mogulkoc R, Baltaci AK, Emsen A, Artac H. The effect of zinc and melatonin supplementation on immunity parameters in breast cancer induced by DMBA in rats. Arch Physiol Biochem 2018;124(3):247-52.
  • Bicer M, Gunay M, Baltaci AK, Uney K, Mogulkoc R, Akil M. Effect of zinc supplementation on lipid peroxidation and lactate levels in rats with diabetes induced by streptozotocin and subjected to acute swimming exercise. Bratisl Lek Listy 2012;113(4):199-205.
  • Stehbens WE Oxidative stress, toxic hepatitis, and antiaxi- dants with particular emphasis on zinc. Exp Mol Pathol 2003;75:265-76.
  • Tapiero H, Tew KD. Trace elements in human physiology and pathology: zinc and metallothioniens. Biomed Phar- macother 2003;57:399–411.
  • Paffenbarger RS, Kampert JB, Lee IM, Hyde RT, Leung RW, Wing AL. Changes in physical activity and other li- feway patterns influencing longevity. Med Sci Sports Exerc 1994;26:857-65.
  • Kim JD, Yu BP, McCarter RJM, Lee SY, Herlihy JT. Exercise and diet modulate cardiac lipid peroxidation and antioxi- dant defenses. Free Radic Biol Med 1996;20:83-8.
  • Powers SK, Hamilton K. Antioxidants and exercise. Clin Sports Med 1999;18:525–36.
  • Laaksonen DE, Atalay M, Niskane L, Uusitupa M, Han- ninen O, Sen CK. Increased resting and exercise-induced oxidative stress in young IDDM men. Diabetes Care 1996; 19: 569-74.
  • Rahimi R, Nikfar S, Larijani B, Abdollahi M. A review on the role of antioxidants in the management of diabetes and its complications. Biomed Pharmacother 2005; 59:365–73.
  • Kuroki T, Isshiki K, King GL. Oxidative stress: the lead or supporting actor in the pathogenesis of diabetic complica- tions. J Am Soc Nephrol 2003;4:216-20.
  • Kanter M. Free radicals, exercise and antioxidant supple- mentation. Proc Nutr Soc 1998;57:9-13.
  • Child RB, Wilkinson DM, Fallowfield JL, Donnelly AE. Elevated serum antioxidant capacity and plasma malondi- aldehyde concentration in response to a simulated half-ma- rathon run. Med Sci Sports Exerc1998;30:1603-7.
  • Jenkins RR. Exercise, oxidative stress, and antioxidants: a review. Int J Sport Nutr 1993;3:356–75.
  • Cordova A, Alvarez-Mon M. Behaviour of zinc in physi- cal exercise: a special reference to immunity and fatigue. Neurosci Biobehav Rev 1995;19:439-45.
  • Duzguner V, Kaya S. Effect of zinc on the lipid peroxidation and the antioxidant defense systems of the alloxan-induced diabetic rabbits. Free Radic Biol Med 2007;42:1481-6.
  • Cao GH, Chen JD. Effects of dietary zinc on free radical generation, lipd peroxidation, and superoxide dismutase in trained mice. Arch Biochem Biophys 1991;291:147-53.
  • Jana K, Samanta PK, Manna I, Ghosh P, Singh N, Khetan RP, Ray BR Protective effect of sodium selenite and zinc sul- fate on intensive swimming-induced testicular gamatoge- nic and steroidogenic disorders in mature male rats. Appl Physiol Nutr Metab 2008;33:903-14.
  • Evans JL, Goldfine ID, Maddux BA, Grodsky GM (2003) Are oxidative stres-activated signaling pathways media- tors of insülin resistance and-cell dysfunction. Diabetes 2003;52:1-8.

Year 2018, Volume: 28 Issue: 3, 102 - 106, 01.09.2018

Mürsel Biçer Sadettin Ünsal

Abstract

Objective: The objective of the present study is to explore the effect of zinc supplementation on lipid peroxidation and antioxidant capacity in the muscle tissue of rats which were induced diabetes with streptozotocin and subjected to acute swimming exercise.Material and Method: The study included 80 adult, male, Spraque-Dawley type rats, which were equally allocated to 8 groups. Group 1, general control. Group 2, zinc-supplemented control. Group 3, zinc-supplemented diabetic control. Group 4, swimming control. Group 5, zinc-supplemented swimming. Group 6, zinc-supplemented diabetic swimming. Group 7, diabetic swimming. Group 8, diabetes group. The animals were injected with 40 mg/kg intraperitoneal ip streptozotocin STZ to induce diabetes. The injections were repeated after 24 hours. The animals whose blood glucose stood at or over 300 mg/dl on day 6 following the last injection were considered diabetic. The animals were supplemented with 6 mg/kg/day ip zinc sulfate for 4 weeks. At the end of the 4-week study, the animals were decapitated to collect muscle tissue samples, which were analyzed in terms of MDA nmol/gram/protein and GSH mg/dl/gram protein levels.Results: The highest MDA values in the muscle tissue were established in groups 4 and 7. Muscle MDA levels in groups 5 and 6 were lower than those in groups 4 and 7, but higher than the levels in all other groups. Groups 5 and 6 had the highest muscle GSH values. Group 4 had this parameter lower than groups 5 and 6, but higher than others. The lowest GSH levels in the muscle tissue were obtained in groups 7 and 8.Conclusion: Results of the study demonstrate that zinc sulfate supplementation has a protective role against lipid peroxidation caused by strenuous swimming exercise in the muscle tissue of diabetic rats

Keywords

Zinc diabetes muscle tissue lipid peroxidation exercise

References

  • Baltaci SB, Mogulkoc R, Baltaci AK. Resveratrol and exerci- se. Biomed Rep 2016; 5(5):525-30.
  • Bicer M, Baltaci SB, Patlar S, Mogulkoc R, Baltaci AK. Me- latonin has a protective effect against lipid peroxidation in the bone tissue of diabetic rats subjected to acute swimming exercise. Horm Mol Biol Clin Investig 2018 Mar 16;34(2). pii:/j/hmbci.2018.34.issue-2/hmbci-2017-0079/hmb- ci-2017-0079.xml.
  • Sivrikaya A, Bicer M, Akil M, Baltaci AK, Mogulkoc R. Ef- fects of zinc supplementation on the element distribution in kidney tissue of diabetic rats subjected to acute swim- ming. Biol Trace Elem Res 2012;147(1-3):195-9.
  • Brandao-Neto J, Silva CAB, Rezende AA, Almeida MG, Sa- les VSP, Marchini JS. Zinc pharmacokinetics in insülin–de- pendent diabetes mellitus patients after oral zinc tolerance test. Nutr Res 2003;23:141–50.
  • Gold G, Grodsky GM. Kinetic aspects of comparrmen- tal storage and secretion of insulin and zinc. Experientia 1984;40:1105 –14.
  • Baltaci SB, Mogulkoc R, Baltaci AK, Emsen A, Artac H. The effect of zinc and melatonin supplementation on immunity parameters in breast cancer induced by DMBA in rats. Arch Physiol Biochem 2018;124(3):247-52.
  • Bicer M, Gunay M, Baltaci AK, Uney K, Mogulkoc R, Akil M. Effect of zinc supplementation on lipid peroxidation and lactate levels in rats with diabetes induced by streptozotocin and subjected to acute swimming exercise. Bratisl Lek Listy 2012;113(4):199-205.
  • Stehbens WE Oxidative stress, toxic hepatitis, and antiaxi- dants with particular emphasis on zinc. Exp Mol Pathol 2003;75:265-76.
  • Tapiero H, Tew KD. Trace elements in human physiology and pathology: zinc and metallothioniens. Biomed Phar- macother 2003;57:399–411.
  • Paffenbarger RS, Kampert JB, Lee IM, Hyde RT, Leung RW, Wing AL. Changes in physical activity and other li- feway patterns influencing longevity. Med Sci Sports Exerc 1994;26:857-65.
  • Kim JD, Yu BP, McCarter RJM, Lee SY, Herlihy JT. Exercise and diet modulate cardiac lipid peroxidation and antioxi- dant defenses. Free Radic Biol Med 1996;20:83-8.
  • Powers SK, Hamilton K. Antioxidants and exercise. Clin Sports Med 1999;18:525–36.
  • Laaksonen DE, Atalay M, Niskane L, Uusitupa M, Han- ninen O, Sen CK. Increased resting and exercise-induced oxidative stress in young IDDM men. Diabetes Care 1996; 19: 569-74.
  • Rahimi R, Nikfar S, Larijani B, Abdollahi M. A review on the role of antioxidants in the management of diabetes and its complications. Biomed Pharmacother 2005; 59:365–73.
  • Kuroki T, Isshiki K, King GL. Oxidative stress: the lead or supporting actor in the pathogenesis of diabetic complica- tions. J Am Soc Nephrol 2003;4:216-20.
  • Kanter M. Free radicals, exercise and antioxidant supple- mentation. Proc Nutr Soc 1998;57:9-13.
  • Child RB, Wilkinson DM, Fallowfield JL, Donnelly AE. Elevated serum antioxidant capacity and plasma malondi- aldehyde concentration in response to a simulated half-ma- rathon run. Med Sci Sports Exerc1998;30:1603-7.
  • Jenkins RR. Exercise, oxidative stress, and antioxidants: a review. Int J Sport Nutr 1993;3:356–75.
  • Cordova A, Alvarez-Mon M. Behaviour of zinc in physi- cal exercise: a special reference to immunity and fatigue. Neurosci Biobehav Rev 1995;19:439-45.
  • Duzguner V, Kaya S. Effect of zinc on the lipid peroxidation and the antioxidant defense systems of the alloxan-induced diabetic rabbits. Free Radic Biol Med 2007;42:1481-6.
  • Cao GH, Chen JD. Effects of dietary zinc on free radical generation, lipd peroxidation, and superoxide dismutase in trained mice. Arch Biochem Biophys 1991;291:147-53.
  • Jana K, Samanta PK, Manna I, Ghosh P, Singh N, Khetan RP, Ray BR Protective effect of sodium selenite and zinc sul- fate on intensive swimming-induced testicular gamatoge- nic and steroidogenic disorders in mature male rats. Appl Physiol Nutr Metab 2008;33:903-14.
  • Evans JL, Goldfine ID, Maddux BA, Grodsky GM (2003) Are oxidative stres-activated signaling pathways media- tors of insülin resistance and-cell dysfunction. Diabetes 2003;52:1-8.
There are 23 citations in total.

Details

Primary Language Turkish
Journal Section Original Article
Authors

Mürsel Biçer This is me

Sadettin Ünsal This is me

Publication Date September 1, 2018
Published in Issue Year 2018 Volume: 28 Issue: 3

Cite

Vancouver Biçer M, Ünsal S. Akut yüzme egzersizi yaptırılan diyabetik sıçanlarda intraperitoneal çinko sülfat uygulamasının kas dokusundaki lipid peroksidasyonuna etkisi. Genel Tıp Derg. 2018;28(3):102-6.

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