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Elit taekwondocularda c vitamini uygulamasının IFN-γ, TNF-α, IL-2 ve IL-6 düzeylerine etkisi

Year 2018, Volume: 28 Issue: 1, 23 - 27, 01.01.2018

Süleyman Patlar Ekrem Boyalı Saltuk Buğra Baltacı Rasim Mogulkoç Abdülkerim Kasım Baltacı

Abstract

Amaç: Bu çalışmanın amacı, taekwondo sporu yapan bireylerde C vitamini uygulamasının sitokin salınımı üzerindeki etkilerinin araştırılmasıdır. Gereç ve Yöntem: Bu araştırma Selçuk Üniversitesi Beden Eğitimi ve Spor Yüksekokulunda okuyan yaş ortalaması; 20.67±0.24 yıl, vücut ağırlığı ortalaması; 65.45±1.69 kg olan 10 adet erkek elit taekwondocu üzerinde gerçekleştirildi. Deneklere 4 hafta süreyle günde oral olarak 300 mg C vitamini tablet şeklinde verildi ve 4 haftalık vitamin ilavesi öncesi ve sonrası olmak üzere iki kez yorgunluk oluşuncaya kadar tükenme egzersizi yaptırıldı. Dört hafta süren uygulama öncesinde ve sonrasında deneklerden istirahat ve yorgunluk olmak üzere 4 kez kan örnekleri alındı. Alınan kan örneklerinde serumda interferon gama IFN-γ , tümör nekrosiz faktör-alfa TNF-α , interlökin-2 IL-2 ve interlökin-6 IL-6 düzeyleri ELISA test kitleri kullanılarak tayin edildi. Bulgular: IFN-γ, TNF-α ve IL-2 düzeyleri hem uygulama öncesi hem de sonrası anlamlı bir farklılık göstermedi p>0.05 . Uygulamadan önceki egzersiz periyodunda serum IL-6 düzeylerinde dinlenme periyoduna göre anlamlı artış vardı p

Keywords

Vitamin C sitokinler egzersiz yorgunluk Taekwondo

References

  • Rodriguez NR, Di Marco NM, Langley S. Nutrition and athletic performance. Med Sci Sports Exerc 2009;41(3):709- 31.
  • Rodriguez NR, DiMarco NM, Langley S. Nutrition and ath- letic performance. J Am Diet Assoc 2009;109(3):509-27.
  • Goldfarb AH, Bloomer RJ, McKenzie MJ. Combined an- tioxidant treatment effects on blood oxidative stress after eccentric exercise. Med Sci Sports Exerc 2005;37(2):234-9.
  • Shephard RJ. The athlete at high altitude. Can Med Assoc J 1973;109(3):207-9.
  • Masek J, Hruba F. On the relation between the saturation of the serum and the leukocytes with vitamin C. Int Z Vita- minforsch 1964;34:39-44.
  • Schrauzer GN, Rhead WJ. Ascorbic acid abuse: effects on long term ingestion of excessive amounts on blood levels and urinary excretion. Int J Vitam Nutr Res 1973;43(2):201- 11.
  • Schrauzer GN, Ishmael D, Kiefer GW. Some aspects of current vitamin C usage: diminished high-altitude resis- tance following overdosage. Ann N Y Acad Sci 1975 Sep 30;258:377-81.
  • Deaton CM, Marlin DJ, Roberts CA, Smith N, Harris PA, Kelly FJ, Schroter RC.Antioxidant supplementation and pulmonary function at rest and exercise. Equine Vet J Suppl 2002;(34):58-65.
  • Aguilo A, Tauler P, Fuentespina E, Villa G, Cordova A, Tur JA, Pons A.Antioxidant diet supplementation influences blood iron status in endurance athletes. Int J Sport Nutr Exerc Metab 2004;14(2):147-60.
  • Aydemir T, Oztürk R, Bozkaya LA, Tarhan L. Effects of antioxidant vitamins A, C, E and trace elements Cu, Se on CuZn SOD, GSH-Px, CAT and LPO levels in chicken eryth- rocytes. Cell Biochem Funct 2000;18(2):109-15.
  • Günay M, Tamer K, Cicioglu I Spor Fizyolojisi ve Perfor- mans Ölçümü. 1. Baskı. Ankara: Gazi Kitabevi, 2006; 538.
  • Marques EA, Mota J, Viana JL, Tuna D, Figueiredo P, Gu- imarães JT, Carvalho J. Response of bone mineral density, inflammatory cytokines, and biochemical bone markers to a 32-week combined loading exercise programme in older men and women. Arch Gerontol Geriatr 2013;57(2):226-33.
  • LaVoy EC, Bosch JA, Lowder TW, Simpson RJ. Acute ae- robic exercise in humans increases cytokine expression in CD27(-) but not CD27(+) CD8(+) T-cells. Brain Behav Im- mun 2013;27(1):54-62.
  • Touvra AM, Volaklis KA, Spassis AT, Zois CE, Douda HD, Kotsa K, Tokmakidis SP. Combined strength and ae- robic training increases transforming growth factor-β1 in patients with type 2 diabetes. Hormones (Athens) 2011;10(2):125-30.
  • Terra R, Alves PJ, Gonçalves da Silva SA, Salerno VP, Dut- ra PM. Exercise improves the Th1 response by modulating cytokine and NO production in BALB/c mice. Int J Sports Med 2013;34(7):661-6.
  • Lamprecht ED, Williams CA. Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affe- cted by acute intense exercise but not superoxide dismu- tase supplementation in horses. Oxid Med Cell Longev 2012;2012:15.
  • Mignot CC, Pirottin D, Farnir F, de Moffarts B, Molitor C, Lekeux P, Art T. Effect of strenuous exercise and ex vivo TLR3 and TLR4 stimulation on inflammatory gene expres- sion in equine pulmonary leukocytes. Vet Immunol Immu- nopathol 2012;147(3-4):127-35.
  • Lu YM, Zhang H, Tang CZ. [Effects of electroacupuncture on IL-2-IFN-NKC immunity immunoloregulation net and IL-2 receptor in rats with exercise stress]. Zhongguo Zhen Jiu 2011;31(9):817-20.
  • Baltaci AK, Mogulkoc R, Bediz CS, Pekel A. Effects of zinc deficiency and pinealectomy on cellular immunity in rats infected with Toxoplasma gondii. Biol Trace Elem Res. 2005;104(1):47-56.
  • Sacheck JM, Cannon JG, Hamada K, Vannier E, Blumberg JB, Roubenoff R. Age-related loss of associations between acute exercise-induced IL-6 and oxidative stress. Am J Phy- siol Endocrinol Metab 2006;291(2):E340-9.
  • Singh A, Papanicolaou DA, Lawrence LL, Howell EA, Ch- rousos GP, Deuster PA. Neuroendocrine responses to run- ning in women after zinc and vitamin E supplementation. Med Sci Sports Exerc 1999;31(4):536-42.
  • Du WD, Yuan ZR, Sun J, Tang JX, Cheng AQ, Shen DM, Huang CJ, Song XH, Yu XF, Zheng SB. Therapeutic efficacy of high-dose vitamin C on acute pancreatitis and its poten- tial mechanisms. World J Gastroenterol 2003;9(11):2565-9.
  • Akolkar G, da Silva Dias D, Ayyappan P, Bagchi AK, Jassal DS, Salemi VMC, Irigoyen MC, De Angelis K, Singal PK. Vitamin C mitigates oxidative/nitrosative stress and inf- lammation in doxorubicin-induced cardiomyopathy. Am J Physiol Heart Circ Physiol 2017;313(4):795-809.
  • Kim H, Kim Y, Bae S, Lim SH, Jang M, Choi J, Jeon J, Hwang YI, Kang JS, Lee WJ.Vitamin C Deficiency Causes Severe Defects in the Development of the Neonatal Cerebellum and in the Motor Behaviors of Gulo(-/-) Mice. Antioxid Re- dox Signal 2015;23(16):1270-83.
  • Fischer CP. Interleukin-6 in acute exercise and training: what is the biological relevance? Exerc Immunol Rev 2006;12:6-33.
  • Aguiló A, Monjo M, Moreno C, Martinez P, Martínez S, Ta- uler P. Vitamin C supplementation does not influence plas- ma and blood mononuclear cell IL-6 and IL-10 levels after exercise. J Sports Sci 2014;32(17):1659-69.
  • Cesari M, Penninx BW, Pahor M, Lauretani F, Corsi AM, Rhys Williams G, Guralnik JM, Ferrucci L. Inflamma- tory markers and physical performance in older persons: the InCHIANTI study. J Gerontol A Biol Sci Med Sci 2004;59(3):242-8.
  • Colbert LH, Visser M, Simonsick EM, Tracy RP, Newman AB, Kritchevsky SB, Pahor M, Taaffe DR, Brach J, Rubin S, Harris TB. Physical activity, exercise, and inflammatory markers in older adults: findings from the Health, Aging and Body Composition Study. J Am Geriatr Soc 2004; 52(7):1098-104.
  • Gokhale R, Chandrashekara S, Vasanthakumar KC. Cytoki- ne response to strenuous exercise in athletes and non-athle- tes—an adaptive response. Cytokine 2007;40(2):123-7.
  • Fischer CP, Hiscock NJ, Penkowa M, Basu S, Vessby B, Kallner A, SjöbergLB, Pedersen BK.Supplementation with vitamins C and E inhibits the release of interleu- kin-6 from contracting human skeletal muscle. J Physiol 2004;558(2):633-45.

Year 2018, Volume: 28 Issue: 1, 23 - 27, 01.01.2018

Süleyman Patlar Ekrem Boyalı Saltuk Buğra Baltacı Rasim Mogulkoç Abdülkerim Kasım Baltacı

Abstract

Objective: The aim of this study was to investigate the effects of vitamin C application on secretion of cytokine in individuals who do taekwondo sport.Materials and Methods: This research was carried out on 10 elite taekwondo athletes who were studying in Selçuk University, School of Physical Education and Sports. Their mean age; 20.67±0.24 years and mean body weight was; 65.45±1.69 kg. Every day 300 mg vitamin C was given to the participants orally during 4 weeks and exhaustive exercise was done until fatigue occurs before and after vitamin E treatment. Before and following the 4 week implementation period, blood samples were taken 4 times from the participants as at rest and fatigue. Interferon gamma IFN-γ , tumour necrosis factor-alpha TNF-α , interleukin -2 IL-2 and interleukin -6 IL6 levels were determined by ELISA test kits on taken blood samples. Results: There were no significant differences on the levels of IFN-γ, IL-2 and TNF-α both before and after implementation. There was significant increase on the levels of serum IL-6 at exercise period in comparison with resting period P

Keywords

Vitamin C Cytokines Exercise Fatigue Taekwondo

References

  • Rodriguez NR, Di Marco NM, Langley S. Nutrition and athletic performance. Med Sci Sports Exerc 2009;41(3):709- 31.
  • Rodriguez NR, DiMarco NM, Langley S. Nutrition and ath- letic performance. J Am Diet Assoc 2009;109(3):509-27.
  • Goldfarb AH, Bloomer RJ, McKenzie MJ. Combined an- tioxidant treatment effects on blood oxidative stress after eccentric exercise. Med Sci Sports Exerc 2005;37(2):234-9.
  • Shephard RJ. The athlete at high altitude. Can Med Assoc J 1973;109(3):207-9.
  • Masek J, Hruba F. On the relation between the saturation of the serum and the leukocytes with vitamin C. Int Z Vita- minforsch 1964;34:39-44.
  • Schrauzer GN, Rhead WJ. Ascorbic acid abuse: effects on long term ingestion of excessive amounts on blood levels and urinary excretion. Int J Vitam Nutr Res 1973;43(2):201- 11.
  • Schrauzer GN, Ishmael D, Kiefer GW. Some aspects of current vitamin C usage: diminished high-altitude resis- tance following overdosage. Ann N Y Acad Sci 1975 Sep 30;258:377-81.
  • Deaton CM, Marlin DJ, Roberts CA, Smith N, Harris PA, Kelly FJ, Schroter RC.Antioxidant supplementation and pulmonary function at rest and exercise. Equine Vet J Suppl 2002;(34):58-65.
  • Aguilo A, Tauler P, Fuentespina E, Villa G, Cordova A, Tur JA, Pons A.Antioxidant diet supplementation influences blood iron status in endurance athletes. Int J Sport Nutr Exerc Metab 2004;14(2):147-60.
  • Aydemir T, Oztürk R, Bozkaya LA, Tarhan L. Effects of antioxidant vitamins A, C, E and trace elements Cu, Se on CuZn SOD, GSH-Px, CAT and LPO levels in chicken eryth- rocytes. Cell Biochem Funct 2000;18(2):109-15.
  • Günay M, Tamer K, Cicioglu I Spor Fizyolojisi ve Perfor- mans Ölçümü. 1. Baskı. Ankara: Gazi Kitabevi, 2006; 538.
  • Marques EA, Mota J, Viana JL, Tuna D, Figueiredo P, Gu- imarães JT, Carvalho J. Response of bone mineral density, inflammatory cytokines, and biochemical bone markers to a 32-week combined loading exercise programme in older men and women. Arch Gerontol Geriatr 2013;57(2):226-33.
  • LaVoy EC, Bosch JA, Lowder TW, Simpson RJ. Acute ae- robic exercise in humans increases cytokine expression in CD27(-) but not CD27(+) CD8(+) T-cells. Brain Behav Im- mun 2013;27(1):54-62.
  • Touvra AM, Volaklis KA, Spassis AT, Zois CE, Douda HD, Kotsa K, Tokmakidis SP. Combined strength and ae- robic training increases transforming growth factor-β1 in patients with type 2 diabetes. Hormones (Athens) 2011;10(2):125-30.
  • Terra R, Alves PJ, Gonçalves da Silva SA, Salerno VP, Dut- ra PM. Exercise improves the Th1 response by modulating cytokine and NO production in BALB/c mice. Int J Sports Med 2013;34(7):661-6.
  • Lamprecht ED, Williams CA. Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affe- cted by acute intense exercise but not superoxide dismu- tase supplementation in horses. Oxid Med Cell Longev 2012;2012:15.
  • Mignot CC, Pirottin D, Farnir F, de Moffarts B, Molitor C, Lekeux P, Art T. Effect of strenuous exercise and ex vivo TLR3 and TLR4 stimulation on inflammatory gene expres- sion in equine pulmonary leukocytes. Vet Immunol Immu- nopathol 2012;147(3-4):127-35.
  • Lu YM, Zhang H, Tang CZ. [Effects of electroacupuncture on IL-2-IFN-NKC immunity immunoloregulation net and IL-2 receptor in rats with exercise stress]. Zhongguo Zhen Jiu 2011;31(9):817-20.
  • Baltaci AK, Mogulkoc R, Bediz CS, Pekel A. Effects of zinc deficiency and pinealectomy on cellular immunity in rats infected with Toxoplasma gondii. Biol Trace Elem Res. 2005;104(1):47-56.
  • Sacheck JM, Cannon JG, Hamada K, Vannier E, Blumberg JB, Roubenoff R. Age-related loss of associations between acute exercise-induced IL-6 and oxidative stress. Am J Phy- siol Endocrinol Metab 2006;291(2):E340-9.
  • Singh A, Papanicolaou DA, Lawrence LL, Howell EA, Ch- rousos GP, Deuster PA. Neuroendocrine responses to run- ning in women after zinc and vitamin E supplementation. Med Sci Sports Exerc 1999;31(4):536-42.
  • Du WD, Yuan ZR, Sun J, Tang JX, Cheng AQ, Shen DM, Huang CJ, Song XH, Yu XF, Zheng SB. Therapeutic efficacy of high-dose vitamin C on acute pancreatitis and its poten- tial mechanisms. World J Gastroenterol 2003;9(11):2565-9.
  • Akolkar G, da Silva Dias D, Ayyappan P, Bagchi AK, Jassal DS, Salemi VMC, Irigoyen MC, De Angelis K, Singal PK. Vitamin C mitigates oxidative/nitrosative stress and inf- lammation in doxorubicin-induced cardiomyopathy. Am J Physiol Heart Circ Physiol 2017;313(4):795-809.
  • Kim H, Kim Y, Bae S, Lim SH, Jang M, Choi J, Jeon J, Hwang YI, Kang JS, Lee WJ.Vitamin C Deficiency Causes Severe Defects in the Development of the Neonatal Cerebellum and in the Motor Behaviors of Gulo(-/-) Mice. Antioxid Re- dox Signal 2015;23(16):1270-83.
  • Fischer CP. Interleukin-6 in acute exercise and training: what is the biological relevance? Exerc Immunol Rev 2006;12:6-33.
  • Aguiló A, Monjo M, Moreno C, Martinez P, Martínez S, Ta- uler P. Vitamin C supplementation does not influence plas- ma and blood mononuclear cell IL-6 and IL-10 levels after exercise. J Sports Sci 2014;32(17):1659-69.
  • Cesari M, Penninx BW, Pahor M, Lauretani F, Corsi AM, Rhys Williams G, Guralnik JM, Ferrucci L. Inflamma- tory markers and physical performance in older persons: the InCHIANTI study. J Gerontol A Biol Sci Med Sci 2004;59(3):242-8.
  • Colbert LH, Visser M, Simonsick EM, Tracy RP, Newman AB, Kritchevsky SB, Pahor M, Taaffe DR, Brach J, Rubin S, Harris TB. Physical activity, exercise, and inflammatory markers in older adults: findings from the Health, Aging and Body Composition Study. J Am Geriatr Soc 2004; 52(7):1098-104.
  • Gokhale R, Chandrashekara S, Vasanthakumar KC. Cytoki- ne response to strenuous exercise in athletes and non-athle- tes—an adaptive response. Cytokine 2007;40(2):123-7.
  • Fischer CP, Hiscock NJ, Penkowa M, Basu S, Vessby B, Kallner A, SjöbergLB, Pedersen BK.Supplementation with vitamins C and E inhibits the release of interleu- kin-6 from contracting human skeletal muscle. J Physiol 2004;558(2):633-45.
There are 30 citations in total.

Details

Primary Language Turkish
Journal Section Original Article
Authors

Süleyman Patlar This is me

Ekrem Boyalı This is me

Saltuk Buğra Baltacı This is me

Rasim Mogulkoç This is me

Abdülkerim Kasım Baltacı This is me

Publication Date January 1, 2018
Published in Issue Year 2018 Volume: 28 Issue: 1

Cite

Vancouver Patlar S, Boyalı E, Baltacı SB, Mogulkoç R, Baltacı AK. Elit taekwondocularda c vitamini uygulamasının IFN-γ, TNF-α, IL-2 ve IL-6 düzeylerine etkisi. Genel Tıp Derg. 2018;28(1):23-7.

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