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Bruselloz Tanısında Yeni Bir Yöntem: Brucella Coombs Gel Test

Year 2016, Volume: 26 Issue: 1, 19 - 22, 01.01.2016

Hatice Türk Dağı Duygu Fındık

Abstract

Amaç: Bruselloz Brucella cinsi bakterilerin etken olduğu, özellikle gelişmekte olan ülkelerde insanlarda ve hayvanlarda yaygın olarak görülen endemik bir zoonozdur. Brusellozun tanısında mikroorganizmanın kültürde üretilmesi altın standart olmasına rağmen izolasyon oranı çok düşüktür. Bu nedenle serolojik yöntemler daha sık kullanılmaktadır. Bu çalışmanın amacı yeni geliştirilen Brucella Coombs Gel testi ile brusellozun serolojik tanısında standart yöntem olan Coombs’lu Standart Tüp Aglütinasyon testini STA karşılaştırmaktır.Gereç ve yöntem: Selçuk Üniversitesi Tıp Fakültesi Hastanesi’nin çeşitli kliniklerinde bruselloz ön tanısı alan 74 hastanın serum örneği çalışmaya dahil edildi. STA Spinreact, İspanya testi 1/40-1/5120 arası dilüsyonlarda çalışıldı. 24 saat inkübasyondan sonra aglütinasyon görülmeyen tüplere Coombs testi uygulandı. Brucella Coombs Gel testi firma önerileri doğrultusunda çalışıldı. Her iki testte de 1/160 ve üstündeki titreler pozitif kabul edildi.Bulgular: Çalışmaya alınan 74 serum örneğinin 56’sında %75.7 Coombs’lu STA testi ile 53’ünde %71.6 Brucella Coombs Gel testi ile pozitiflik saptanmıştır. Coombs’lu STA testi ile karşılaştırıldığında Brucella Coombs Gel testinin duyarlılığı %94.6, özgüllüğü %100, pozitif prediktif değeri %100 ve negatif prediktif değeri %85.7 olarak bulunmuştur. Sonuç: Sonuç olarak bu çalışmada, Brucella Coombs Gel testinin brusellozun tanısında ve takibinde kullanılan serolojik testlerden Coombs testi ile benzer performansa sahip olduğu görülmüştür

Keywords

Bruselloz Brucella Coombs Gel test Coombs Standart tüp aglütinasyon

References

  • EJ Y. Brucella species. In: Mandell GL, Bennet JE, Dolin R, eds. Principles and Practice of Infectious Diseases. 6th ed. New York: Churchill Livingstone, 2005: 2669-72.
  • Hussein MZ, Abou-Elnoeman SA, Al-Fikky AA, El-Sama- douny EI, Shaaban AAM. Evaluation of Rose Bengal Test, Standard Tube Agglutination Test and Nested PCR for the diagnosis of Human Brucellosis. Egypt J Med Microbiol 2006;15:249-56.
  • Seleem MN, Boyle SM, Sriranganathan N. Brucellosis: a re-emerging zoonosis. Vet Microbiol 2010;140:392-8.
  • Araj GF. Update on laboratory diagnosis of human brucel- losis. Int J Antimicrob Agents 2010;36:12-7.
  • Aliskan H. The value of culture and serological metho- ds in the diagnosis of human brucellosis. Mikrobiyol Bul 2008;42:185-95.
  • Vrioni G, Pappas G, Priavali E, Gartzonika C, Levidiotou S. Aneternal microbe: Brucella DNA load persists for years after clinical cure. Clin Infect Dis 2008;46:e131-6.
  • Maas KS, Mendez M, Zavaleta M, et al. Evaluation of bru- cellosis by PCR and persistence after treatment in patients returning to the hospital for follow-up. Am J Trop Med Hyg 2007;76:698-702.
  • Gomez MC, Nieto JA, Rosa C, et al. Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic. Clin Vaccine Immunol 2008;15:1031-3.
  • Asaad AM, Alqahtani JM. Serological and molecular diag- nosis of human brucellosis in Najran, Southwestern Saudi Arabia. J Infect Public Health 2012;5:189-94.
  • Poester FP, Nielsen K, Samartino LE, Yu WL. Diagnosis of brucellosis. Open Vet Sci J 2010;4:46-60.
  • Patra KP, Saito M, Atluri VL, et al. A protein-conjugate ap- proach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. PLoS Negl Trop Dis 2014;8:e2926.
  • Bayram Y, Korkoca H, Aypak C, et al. Antimicrobial sus- ceptibilities of Brucella isolates from various clinical speci- mens. Int J Med Sci 2011; 8:198-202.
  • Ulu Kiliç A, Metan G, Alp E. Clinical Presentations and Di- agnosis of Brucellosis. Recent Pat Antiinfect Drug Discov 2013;8:34-41.
  • Nielsen K, Yu WL. Serological diagnosis of brucellosis. Pri- lozi 2010; 31:65-89.
  • Al Dahouk S, Sprague LD, Neubauer H. New developments in the diagnostic procedures for zoonotic brucellosis in hu- mans. Rev Sci Tech 2013;32:177-88.
  • Mantur BG, Amarnath SK, Parande AM, et al. Compari- son of a novel immunocapture assay with standard sero- logical methods in the diagnosis of brucellosis. Clin Lab 2011;57:333-41.
  • Ozdemir M, Feyzioglu B, Kurtoglu MG, Dogan M, Dagi HT, Yuksekkaya S, et al. A comparison of immuncapture agglutination and ELISA methods in serological diagnosis of brucellosis. Int J Med Sci 2011;8:428-32.
  • Fadeel MA, Hoffmaster AR, Shi J, Pimentel G, Stoddard RA. Comparison of four commercial IgM and IgG ELISA kits for diagnosing brucellosis. J Med Microbiol 2011;60:1767- 73.
  • Pabuccuoglu O, Ecemis T, El S, Coskun A, Akcali S, Sanli- dag T. Evaluation of serological tests for diagnosis of brucel- losis. Jpn J Infect Dis 2011;64:272-6.

Year 2016, Volume: 26 Issue: 1, 19 - 22, 01.01.2016

Hatice Türk Dağı Duygu Fındık

Abstract

Objectives: Brucellosis caused by Brucella genus is an endemic zoonosis which is widely seen in humans and animals especially in developing countries. Although the growth of the microorganism in culture is a gold standard in diagnosis of brucellosis, the isolation rate is very low. Thus, the serological methods are commonly used. This study aims to compare the newly developed Brucella Coombs Gel test with standard tube agglutination STA test with Coombs that is the standard method in the serological diagnosis of brucellosis.Material and methods: The serum samples of 74 patients diagnosed with brucellosis in the various clinics of Selcuk University, Faculty of Medicine Hospital were included in the study. STA test Spinreact, Spain was studied between 1/40-1/5120 dilutions. After 24 hours of incubation, Coombs test was applied to the tubes invisible agglutination. Brucella Coombs Gel test was performed in accordance with companies recommendations. >1/160 titers were considered as positive in both tests. Results: 56 75.7% and 53 71.6% of 74 patients included in the study were positive in STA test with Coombs and Brucella Coombs Gel test respectively. When compared to STA test with Coombs, the sensitivity, specificity, positive predictive and negative predictive values of Brucella Coombs Gel test were 94.6%, 100%, 100% and 85.7% respectively.Conclusion: Brucella Coombs Gel test has performance similar to Coombs test is serological test used in the diagnosis and monitorng of brucellosis

Keywords

Brucellosis Brucella Coombs Gel test Coombs Standard tube agglutination

References

  • EJ Y. Brucella species. In: Mandell GL, Bennet JE, Dolin R, eds. Principles and Practice of Infectious Diseases. 6th ed. New York: Churchill Livingstone, 2005: 2669-72.
  • Hussein MZ, Abou-Elnoeman SA, Al-Fikky AA, El-Sama- douny EI, Shaaban AAM. Evaluation of Rose Bengal Test, Standard Tube Agglutination Test and Nested PCR for the diagnosis of Human Brucellosis. Egypt J Med Microbiol 2006;15:249-56.
  • Seleem MN, Boyle SM, Sriranganathan N. Brucellosis: a re-emerging zoonosis. Vet Microbiol 2010;140:392-8.
  • Araj GF. Update on laboratory diagnosis of human brucel- losis. Int J Antimicrob Agents 2010;36:12-7.
  • Aliskan H. The value of culture and serological metho- ds in the diagnosis of human brucellosis. Mikrobiyol Bul 2008;42:185-95.
  • Vrioni G, Pappas G, Priavali E, Gartzonika C, Levidiotou S. Aneternal microbe: Brucella DNA load persists for years after clinical cure. Clin Infect Dis 2008;46:e131-6.
  • Maas KS, Mendez M, Zavaleta M, et al. Evaluation of bru- cellosis by PCR and persistence after treatment in patients returning to the hospital for follow-up. Am J Trop Med Hyg 2007;76:698-702.
  • Gomez MC, Nieto JA, Rosa C, et al. Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic. Clin Vaccine Immunol 2008;15:1031-3.
  • Asaad AM, Alqahtani JM. Serological and molecular diag- nosis of human brucellosis in Najran, Southwestern Saudi Arabia. J Infect Public Health 2012;5:189-94.
  • Poester FP, Nielsen K, Samartino LE, Yu WL. Diagnosis of brucellosis. Open Vet Sci J 2010;4:46-60.
  • Patra KP, Saito M, Atluri VL, et al. A protein-conjugate ap- proach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. PLoS Negl Trop Dis 2014;8:e2926.
  • Bayram Y, Korkoca H, Aypak C, et al. Antimicrobial sus- ceptibilities of Brucella isolates from various clinical speci- mens. Int J Med Sci 2011; 8:198-202.
  • Ulu Kiliç A, Metan G, Alp E. Clinical Presentations and Di- agnosis of Brucellosis. Recent Pat Antiinfect Drug Discov 2013;8:34-41.
  • Nielsen K, Yu WL. Serological diagnosis of brucellosis. Pri- lozi 2010; 31:65-89.
  • Al Dahouk S, Sprague LD, Neubauer H. New developments in the diagnostic procedures for zoonotic brucellosis in hu- mans. Rev Sci Tech 2013;32:177-88.
  • Mantur BG, Amarnath SK, Parande AM, et al. Compari- son of a novel immunocapture assay with standard sero- logical methods in the diagnosis of brucellosis. Clin Lab 2011;57:333-41.
  • Ozdemir M, Feyzioglu B, Kurtoglu MG, Dogan M, Dagi HT, Yuksekkaya S, et al. A comparison of immuncapture agglutination and ELISA methods in serological diagnosis of brucellosis. Int J Med Sci 2011;8:428-32.
  • Fadeel MA, Hoffmaster AR, Shi J, Pimentel G, Stoddard RA. Comparison of four commercial IgM and IgG ELISA kits for diagnosing brucellosis. J Med Microbiol 2011;60:1767- 73.
  • Pabuccuoglu O, Ecemis T, El S, Coskun A, Akcali S, Sanli- dag T. Evaluation of serological tests for diagnosis of brucel- losis. Jpn J Infect Dis 2011;64:272-6.
There are 19 citations in total.

Details

Primary Language Turkish
Journal Section Original Article
Authors

Hatice Türk Dağı This is me

Duygu Fındık This is me

Publication Date January 1, 2016
Published in Issue Year 2016 Volume: 26 Issue: 1

Cite

Vancouver Dağı HT, Fındık D. Bruselloz Tanısında Yeni Bir Yöntem: Brucella Coombs Gel Test. Genel Tıp Derg. 2016;26(1):19-22.

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