Effects of curcumin supplementation on oxidant/antioxidant status of heart tissue in aged rats

Year 2012, Volume: 22 Issue: 2, 61 - 66, 01.01.2012

Muaz Belviranlı Nilsel Okudan Kısmet Esra Nurullahoğlu Atalık

Abstract

Objective: The aim of the study was to investigate the effects of curcumin supplementation on oxidative stress and antioxidant defense in heart tissue of aged female rats. Methods: Twenty aged female rats aged twenty months were randomly divided into two grops: control and curcumin. In curcumin supplemented group curcumin was given at 300 mg/kg dosage for 12 days via oral gavage dissolved in corn oil. Twenty-four hour after the last supplementation heart tissues were taken under anesthesia and malondialdehyde MDA , protein carbonyl PC and glutathione GSH levels were analyzed in these samples. Results: Curcumin supplementation significantly elevated GSH levels P0.05 . Conclusion: Curcumin supplementation protects the heart against oxidative damage and it improves antioxidant defense system in aged female rats

Keywords

Curcumin, aging, oxidative stress, heart

Yaşlı sıçanlarda kurkumin takviyesinin kalp dokusunun oksidan/antioksidan durumu üzerine etkileri

Abstract

Amaç: Çalışmanın amacı kurkumin takviyesinin yaşlı dişi sıçanların kalp dokusunda oksidatif stres ve antioksidan savunma üzerine etkilerini araştırmaktı. Yöntem: Çalışmada 20 aylık 20 dişi Wistar sıçan kontrol ve kurkumin grupları olmak üzere rastgele 2 gruba ayrıldı. Kurkumin grubuna 12 gün boyunca günde 300 mg/kg dozunda kurkumin mısır yağı içerisinde çözülerek oral gavaj yoluyla verildi. Son takviyeden 24 saat sonra anestezi altında kalp dokuları alındı ve alınan örneklerde malondialdehit MDA , protein karbonil PC ve glutatyon GSH seviyeleri analiz edildi. Bulgular: Kurkumin takviyesi GSH seviyelerini önemli ölçüde artırmıştır P0,05 . Sonuç: Kurkumin takviyesi yaşlı dişi sıçanlarda kalp dokusunu oksidatif hasara karşı korur ve antioksidan savunma sistemini güçlendirir

Keywords

Kurkumin, yaşlanma, oksidatif stres, kalp

References

• Johnson FB, Sinclair DA, Guarente L. Molecular biology of aging. Cell. 1999;96:291-302.
• Dkhar P, Sharma R. Effect of dimethylsulphoxide and curcumin on protein carbonyls and reactive oxygen species of cerebral hemispheres of mice as a function of age. Int J Dev Neurosci. 2010;28:351-7.
• Harman D. Aging: A theory based on free radical and radiation chemistry. J Gerontol. 1956;11:298-300.
• Çakatay U, Telci A, Yılmaz İA, Akçay T, Sivas A. Yaşlanmanın plazma oksidatif protein hasarına etkisi. Cerrahpaşa Tıp Derg. 2000;31:220-3.
• Sastre J, Pallardó FV, Viña J. Mitochondrial oxidative stress plays a key role in aging and apoptosis. IUBMB Life. 2000;49:427-35.
• Boveris A, Chance B. The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen. Biochem J. 1973;134:707-16.
• Lee HC, Wei YH. Mitochondria and aging. Adv Exp Med Biol. 2012;942:311-27.
• Ago T, Matsushima S, Kuroda J, Zablocki D, Kitazono T, Sadoshima J. The NADPH oxidase Nox4 and aging in the heart. Aging (Albany NY). 2010;2:1012-6.
• Doria E, Buonocore D, Focarelli A, Marzatico F. Relationship between human aging muscle and oxidative system pathway. Oxid Med Cell Longev. 2012;830257:1-12
• Garcia-Fernandez M, Sierra I, Puche JE, Guerra L, Castilla- Cortazar I. Liver mitochondrial dysfunction is reverted by insulin-like growth factor II (IGF-II) in aging rats. J Transl Med. 2011;9:123.
• Matthews RT, Yang L, Browne S, Baik M, Beal F. Coenzyme Q10 adminstration increases brain mitochondrial concentrations and exerts neuroprotective effects. Proc Natl Acad Sci. 1998;95:8892–7.
• Suh JH, Shigeno ET, Morrow JD, Cox B, Rocha AE, Frei B, et al. Oxidative stress in the aging rat heart is reversed by dietary supplementation with (R)-a-lipoic acid. Faseb J. 2001;15:700-6.
• Binienda ZK. Neuroprotective effects of L-carnitine in induced mitochondrial dysfunction. Ann NY Acad Sci. 2003;993:289-95.
• Cherniack EP. The potential influence of plant polyphenols on the aging process. Forsch Komplement med. 2010;17:181-7.
• Ferrari CK. Functional foods and physical activities in health promotion of aging people. Maturitas. 2007;58:327-39.
• Naik SR, Thakare VN, Patil SR. Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats: evidence of its antioxidant property. Exp Toxicol Pathol. 2011;63:419-31.
• Reddy AC, Lokesh BR. Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron. Mol Cell Biochem. 1994;137:1-8.
• Sreejayan, Rao MN. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol. 1997;49:105-7.
• Miriyala S, Panchatcharam M, Rengarajulu P. Cardioprotective effects of curcumin. Adv Exp Med Biol. 2007;595:359-77.
• Duan W, Yang Y, Yan J, Yu S, Liu J, Zhou J, et al. The effects of curcumin post-treatment against myocardial ischemia and reperfusion by activation of the JAK2/STAT3 signaling pathway. Basic Res Cardiol. 2012;107:263.
• Srivastava G, Mehta JL. Currying the heart: curcumin and cardioprotection. J Cardiovasc Pharmacol Ther. 2009;14:22-7.
• Nirmala C, Anand S, Puvanakrishnan R. Curcumin treatment modulates collagen metabolism in isoproterenol induced myocardial necrosis in rats. Mol Cell Biochem. 1999;197:31- 7.
• Venkatesan N. Curcumin attenuation of acute adriamycin myocardial toxicity in rats. Br J Pharmacol. 1998;124:425-7.
• Yeh CH, Chen TP, Wu YC, Lin YM, Jing Lin P. Inhibition of NFkappaB activation with curcumin attenuates plasma inflammatory cytokines surge and cardiomyocytic apoptosis following cardiac ischemia/reperfusion. J Surg Res. 2005;125:109-16.
• Morimoto T, Sunagawa Y, Kawamura T, Takaya T, Wada H, Nagasawa A, et al. The dietary compound curcumin inhibits p300 histone acetyltransferase activity and prevents heart failure in rats. J Clin Invest. 2008;118:868-78.
• Bala K, Tripathy BC, Sharma D. Neuroprotective and anti- ageing effects of curcumin in aged rat brain regions. Biogerontology. 2006;7:81-9.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-75.
• Nakao C, Ookawara T, Kizaki T, Oh-Ishi S, Miyazaki H, Haga S, et al. Effects of swimming training on three superoxide dismutase isoenzymes in mouse tissues. J Appl Physiol. 2000;88:649-54.
• Jenkins RR. Exercise, oxidative stress, and antioxidants: A review. Int J Sport Nutr. 1993;3:356-75.
• Parildar H, Dogru-Abbasoglu S, Mehmetçik G, Ozdemirler G, Koçak-Toker N, Uysal M. Lipid peroxidation potential and antioxidants in the heart tissue of beta-alanine- or taurine- treated old rats. J Nutr Sci Vitaminol (Tokyo). 2008;54:61-5.
• Wongcharoen W, Phrommintikul A. The protective role of curcumin in cardiovascular diseases. Int J Cardiol. 2009;133:145-51.
• Kakarla P, Vadluri G, Reddy KS, Leeuwenburgh C. Vulnerability of the mid aged rat myocardium to the age- induced oxidative stress: influence of exercise training on antioxidant defense system. Free Radic Res. 2005;39:1211-7.
• Nazam Ansari M, Bhandari U, Pillai KK. Protective role of curcumin in myocardial oxidative damage induced by isoproterenol in rats. Hum Exp Toxicol. 2007;26:933-8.
• Thiyagarajan M, Sharma SS. Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats. Life Sci. 2004;74:969-85.
• Zhao J, Zhao Y, Zheng W, Lu Y, Feng G, Yu S. Neuroprotective effect of curcumin on transient focal cerebral ischemia in rats. Brain Res. 2008;1229:224-32.