Sıçan Epigastrik Ada Fleplerinde Edaravonun İskemi-Reperfüzyon Hasarına Karşı Koruyucu Etkisi: Deneysel Bir Çalışma

Year 2023, Volume: 33 Issue: 2, 117 - 122, 30.04.2023

Ovunc Akdemir , Burak Ergün Tatar , Yiğit Uyanıkgil , Oytun Erbaş , Feng Zhang , William C. Lineaweaver

https://doi.org/10.54005/geneltip.1181620

Cited By: 1

Abstract

Amaç İskemi-reperfüzyon (I/R) hasarı, iskemik fazdan sonra kan dokuya geri döndüğünde meydana gelen doku hasarıdır. Reperfüzyon, hücre ölümü, doku bozukluğu ve nekroz ile sonuçlanan bir dizi akut inflamatuar süreç başlatır. Edaravone (3-meth-1-pheny1-2-pyrazolin-5-one) güçlü ve benzersiz bir sentetik radikal temizleyicidir. Bu araştırmada, edaravonun İ/R hasarı üzerindeki etkileri araştırıldı.

Gereç ve Yöntemler: 16 yetişkin erkek Sprague-Dawley sıçanı kullanıldı. Sekiz sıçan rastgele iki gruba ayrıldı. Grup 1 (kontrol grubu), tedavi olmaksızın 10 saat boyunca karın derisi flebine iskemi ve reperfüzyonu uygulandı. Grup 2'ye (tedavi grubu) iskemiden 10 saat önce intraperitoneal 3 mg/kg edaravon enjeksiyonu ve karın derisi flebinde reperfüzyon uygulandı. Planimetri kullanılarak flepler 1, 3, 7 ve 10 günlük aralıklarla incelendi. Daha sonra biyokimyasal (doku glutatyonu [GSH], doku proteini, lipid peroksidasyonu [MDA] ve nitrik oksit [NO] düzeylerinin ölçümü) ve histopatolojik (doku glutatyonu [GSH], doku proteini, lipid peroksidasyonu ölçümü) için flepler çıkarıldı. MDA] ve nitrik oksit [NO] seviyeleri)
Bulgular: Grup 1 ve 2 arasında NO, MDA, GSH veya planimetrik analizler açısından anlamlı bir değişiklik (p > 0.05) bulamadık. Grup 2, grup 1'den daha düşük nötrofil sayısına sahipti, ancak fark istatistiksel olarak anlamlı değildi (p > 0.05).
Sonuç Edaravone çok etkili bir antioksidandır. Yine de araştırmamız, bir deri flep modelinde I/R hasarını etkilemeyebileceğini gösteriyor.

Keywords

edaravon, iskemi reperfüzyon hasarı, epigastrik flap

Preventive Effect of Edaravone Against Ischemia-Reperfusion Injury in Rat Epigastric Island Flaps: An Experimental Study

Abstract

Objective Ischemia-reperfusion (I/R) injury is tissue damage occurring when blood returns to a tissue after an ischemic phase. Reperfusion initiates a cascade of acute inflammatory processes that end in cell death, tissue malfunction, and necrosis. Edaravone (3-methy-1-pheny1-2-pyrazolin-5-one) is a powerful and unique synthetic radical scavenger. In this research, the effects of edaravone on I/R damage were investigated.

Material and Methods: 16 adult male Sprague–Dawley rats were utilized. Eight rats were allocated at random into two groups. Group 1 (the control group) experienced ischemia and reperfusion of an abdominal skin flap for 10 hours without therapy. Group 2 (treatment group) received an intraperitoneal injection of 3 mg/kg edaravone 10 hours prior to ischemia and reperfusion in an abdominal skin flap. Using planimetry, flaps were examined at intervals of 1, 3, 7, and 10 days. Then, flaps were removed for biochemical (measurement of tissue glutathione [GSH], tissue protein, lipid peroxidation [MDA], and nitric oxide [NO] levels) and histopathological (measurement of tissue glutathione [GSH], tissue protein, lipid peroxidation [MDA], and nitric oxide [NO] levels)
Results: We found no significant changes (p > 0.05) between groups 1 and 2 in terms of NO, MDA, GSH, or planimetric analyses. Group 2 had a lower neutrophil count than group 1, however the difference was not statistically significant (p > 0.05).
Conclusion Edaravone is a very effective antioxidant. Nevertheless, our research indicates that it may not influence I/R damage in a skin flap model.

Keywords

epigastric flap, edaravone, ischemia reperfusion injury

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