Deneysel Künt Toraks Travması Modelinde Serum Clara Hücresi Salgı Proteini Düzeyinin Tanısal Değeri

Year 2024, Volume: 34 Issue: 5, 660 - 665, 31.10.2024

Hasan Kara , Aysegül Bayır , Ahmet Ak , Yeşim Şerife Bayraktar , Atilla Can , Bahadir Ozturk , Nejat Ünlükal , Fatih Akbuğa , Abdullah Akkuş , Muhammed Eseroğlu

https://doi.org/10.54005/geneltip.1492901

Abstract

Amaç: Künt toraks travması akciğerlerde ölümcül olabilen hasara yol açan önemli bir klinik problemdir. Deneysel künt toraks travması hayvan modelleriyle iyi bir şekilde ortaya konmuştur. Bu çalışmada deneysel olarak oluşturulan künt toraks travması modelinde Clara hücresi salgı proteininin (CC16) biyobelirteç olarak kullanımının araştırılması amaçlandı.
Gereç ve Yöntemler: Çalışmamızda toplam 30 adet tavşan kullanıldı. Farklı düzeylerde pulmoner kontüzyon oluşturmak için modifiye edilmiş iki taraflı künt toraks travma modeli kullanıldı. Künt toraks travmasının uygulandığı enerji düzeyine göre tavşanları dört gruba ayırdık. Kontrol ve travma gruplarından 0, 12 ve 24. saatlerde CC16 düzeylerini değerlendirmek için kan örnekleri alındı.
Bulgular: Deneyin başlangıcında ölçülen CC16 seviyeleri kontrol ve düşük enerji gruplarında orta ve yüksek enerji gruplarına göre anlamlı derecede düşüktü (p =.002). 12. saatte ölçülen CC16 düzeyleri arasında anlamlı fark bulunurken (p=0,004), 24. saatte gruplar arasında anlamlı fark bulunamadı. Grupların CC16 düzeylerinin zaman içindeki değişimini incelediğimizde CC16 düzeylerinin 0-12. saatlerde azaldığını, 12. saatten sonra ise arttığını gözlemledik. Histopatolojik olarak farklı gruplarda travmanın ciddiyeti ile orantılı olarak kontüzyon seviyesinin arttığını gözlemledik.
Sonuç: Tasarlanan platformla tavşanlarda künt toraks travmasından kaynaklanan pulmoner kontüzyonun tekrarlanabilir deneysel bir modelini oluşturduk. Pulmoner kontüzyonu takiben artan CC16 seviyeleri, klinik karar vermenin temelini oluşturabilir. Bu nedenle CC16, akut travmatik akciğer hasarı için hızlı ve basit bir biyokimyasal gösterge olarak kullanılma potansiyeline sahiptir.

Keywords

Deneysel, Künt toraks travması, Biyobelirteç, CC16

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Diagnostic Value of Serum Clara Cell Secretory Protein Level in an Experimental Blunt Chest Trauma Model

Abstract

Objective: Blunt chest trauma is a significant clinical problem leading to injury of the lungs that may be fatal. Experimental blunt chest trauma is well established in animal models. This study aimed to investigate whether Clara cell secretory protein (CC16) can be a biomarker in an experimentally created blunt chest trauma model.
Material and Methods: A total of 30 rabbits were used in our study. A modified bilateral blunt thoracic trauma model was used to produce different levels of lung contusion. We divided the rabbits into four groups according to the energy level at which blunt thoracic trauma was applied. Blood samples were taken from the control and trauma groups to evaluate CC16 levels at 0, 12, and 24 hours.
Results: The CC16 levels measured at the start of the experiment were significantly lower in the control and low-energy groups compared to the medium- and high-energy groups (p =.002). While there was a significant difference in CC16 levels measured at the 12th hour (p =.004), no significant difference was found among the groups at the 24th hour. Upon analyzing the change in CC16 levels over time within the groups, we observed that CC16 levels decreased from 0–12 hours and subsequently increased after the 12th hour. Histopathologically, we observed that the level of contusion increased in proportion to the severity of trauma across the different groups.
Conclusion: With the designed platform, we created a reproducible experimental model of pulmonary contusion from blunt thoracic trauma in rabbits. Increased levels of CC16 following a lung contusion could serve as a foundation for clinical decision-making. Thus, CC16 has the potential to serve as a rapid and simple biochemical indicator for acute traumatic lung injury.

Keywords

Experimental, Blunt chest trauma, Biomarker, CC16

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