Evaluation of UCA1/miR-138/CDK6 Network in the Patients with Laryngeal Squamous Cell Carcinoma

Year 2024, Volume: 34 Issue: 5, 666 - 670, 30.10.2024

Neslihan Cinkara , Çiğdem Yüce Kahraman , Arzu Tatar , Abdulgani Tatar

https://doi.org/10.54005/geneltip.1493371

Abstract

Objective: Noncoding RNAs (ncRNAs) have the potential to be diagnostic and therapeutic targets, in many cancer types. miRNA ‐ mRNA ceRNA (competing endogenous RNA) network in cancer has been an interesting topic that has been studied in recent years.
We aimed to investigate the relationship of UCA1/miR-138/CDK6 network with laryngeal squamous cell carcinoma (LSCC).
Material and Methods: We studied with the cancerous tissue and as the control; neighboring normal tissue samples of the patients with diagnosis of LSCC. UCA1, miR-138 and CDK6 expression analysis was made by quantitative real-time PCR (qRT-PCR) method after RNA isolation of the samples.
Results: Expressions of cancerous tissue samples and normal adjacent tissue samples as controls were compared. CDK6 and UCA1 levels were increased and miR-138 levels were found to be decreased in cancer tissues but not statistically significant.
Conclusions: To the best of our knowledge there is no study to provide an expression profile of the UCA1/CDK6/miR-138 network for LSCC patients. Although we could not obtain statistically significant results, our results were similar with the UCA1, miR‐138, and CDK6 axis literature. Further studies with larger patient samples and LSCC cell lines may confirm the function of this axis, which might be a diagnostic and therapeutic target for LSCC.

Keywords

Long non-coding RNA, lncRNA, Laryngeal cancer, miRNA, UCA1, miR138, CDK6

Ethical Statement

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of ??? University (B.30.2.???.0.01.00/60). Written informed consent was obtained from all participants.

Supporting Institution

Ataturk University BAP coordinatory unit

Project Number

TTU- 2021-9277

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