Overview of Cryptosporidium spp.

Year 2025, Volume: 35 Issue: 1, 212 - 218, 28.02.2025

Duygu Beder

https://doi.org/10.54005/geneltip.1469783

Abstract

Abstract
Cryptosporidium spp., the causative agent of Cryptosporidiosis, is an obligate intracellular and extracytoplasmic protozoan. Cryptosporidium spp., emerges as a public health problem transmitted by contaminated water and food due to its features such as the widespread occurrence of oocysts in nature, low infective doses, ability to pass through the filters of treatment plants, resistance to disinfectants, and ability to survive in water and soil for months at appropriate humidity and temperature. Transmission to humans usually occurs through the fecal-oral route by ingestion of oocysts. However, endogenous auto-infection can ocur. Respiratory transmission has been reported. Cryptosporidium spp. infections may lead to serious life-threatening clinical conditions in children under two years of age and immunosuppressed patients. In order to prevent water and foodborne cryptosporidiosis outbreaks and protect public health, the causes of Cryptosporidium oocysts contaminating these resources should be determined, necessary precautions should be taken and combat methods should be determined. In this review, information on the life cycle, epidemiology, clinical findings, diagnosis, protection, and control of Cryptosporidium spp. is presented.

Keywords

cryptosporidium, protozoan, immunosuppressive

References

• 1. Laksemi DA, Suwanti LT, Mufasirin M, Suastika K, Sudarmaja M. Opportunistic Parasitic Infections in Patients with Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome: A Review. Vet World 2019; 13(4): 716-25.
• 2. Leder K, Weller PF. Epidemiology, clinical manifestations, and diagnosis of cryptosporidiosis. Available from:URL:https://www.uptodate.com/contents/epidemiology-clinical-manifestations-and-diagnosis-ofcryptosporidiosis.
• 3. Yalçın S, Doğan NY. Determination of Cryptosporidium spp by Molecular Methods in Different Water Resources of Erzincan Province. Erzincan University Journal of Science and Technology 2019; 12(1): 1-13.
• 4. Wang P, Li S, Zou Y, Du ZC, Song DP, Wang P, et al. The Infection and Molecular Characterization of Cryptosporidium spp. in Diarrheic Pigs in Southern China. Microb Pathog 2022; 165: 105459.
• 5. Şahin S, Ağaoğlu S, Alemdar S. Cryptosporidium ve Cryptosporidiosis. Türkiye Klinikleri; 2018; 35-41.
• 6. Ulusan Bağcı Ö, Caner A. miRNA Expression Profile in Ileocecal Adenocarcinoma Cells Infected with Cryptosporidium. Mikrobiyol Bul 2022; 56(3): 449-465.
• 7. Aksoy Ü. Bioterorism, Prasites as Potential Bioterrorism Agents and Biosecurity studies. Mikrobiyol Bul 2006; 40: 129-139.
• 8. Fayer R. General biology. In: Fayer R, Xiao L. eds. Cryptospsoridium and Cryptosporidiosis. Boca Raton: CRC Press; 2008; 1-42.
• 9. Chalmers RM, Davies AP. Clinical cryptosporidiosis. Exp Parasitol 2010; 124(1): 138- 46.
• 10. Caccio SM, Putignani L. Epidemiology of Human Cryptosporidiosis. In: Caccio S, Widmer G, eds. Cryptosporidium: parasite and disease. Vienna: Springer; 2014; 43-79.
• 11. Ryan U, Hijjawi N, Xiao L. Foodborne cryptosporidiosis. Int J Parasitol 2018; 48(1): 1-12.
• 12. Vanathy K, Parija SC, Mandal J, Hamide A, Krishnamurthy S. Cryptosporidiosis: A mini review. Trop Parasitol 2017; 7(2): 72-80.
• 13. Atasever M, et al. Investigating the Quality of Public Fountain Water in Gümüşhane Province and the Presence of Cryptosporidium spp. Using the PCR Method. Turkish Journal of Agriculture-Food Science and Technology 2024; 12(9): 1530-1538.
• 14. Tin D, Sabeti P, Ciottonea GR. Bioterrorism: An Analysis of Biological Agents Used in Terrorist Events. Am J Emerg Med 2022; 54: 117-121
• 15. Ruh E, Özkan AT. Outbreaks Due to Parasites: Examples from the World and Türkiye. Mikrobiyol Bul 2023, 57(2): 317-329.
• 16. Çuhadar V, Şengül M, Mete E. Investigation of Cryptosporidium parvum in Water Resources in Mardin Province. Turk Mikrobiyol Cemiy Derg 2023; 53(3): 156–162.
• 17. Akkaş Ö, Gürbüz E, Aydemir S, Şahin M, Ekici A. Investigation of Giardia spp., Cryptosporidium spp. and Cyclospora cayetanensis in Samples Collected from Different Spring Waters Iğdır, Türkiye. Turkiye Parazitol Derg 2023;47(2):71-7.
• 18. Macpherson CN, Gottstein B, Geerts S. Parasitic food-borne and water-borne zoonoses. Rev Sci Tech 2000; 19(1): 240-58.
• 19. Şen ZS, et al. A Rare Complication of Acute Diarrhae Caused by Cryptosporidium: Possible Hepatobiliary System Involvement in a Child without Immunodeficiency. Mikrobiyol Bul 2019; 53(4): 464-471.
• 20. Shrivastava AK, Kumar S, Smith WA, Sahu PS. Revisiting the global problem of cryptosporidiosis and recommendations. Trop Parasitol 2017;7(1):8-17.
• 21. Rodrigues F, Davies EG, Harrison P, McLauchlin J, Karani J, Portmann B, et al. Liver disease in children with primary immunodeficiencies. J Pediatr 2004; 145(3): 333-9.
• 22. Miman Ö, Saygı G. Temel Tibbi Parazitoloji. Istanbul: Istanbul Bookstore. 2018:241-248.
• 23. Dabas A, Shah D, Bhatnagar S, Lodha R. Epidemiology of Cryptosporidium in pediatric diarrheal illnesses. Indian Pediatrics 2017; 54(4): 299-309.
• 24. Bensen R, Fuentebella J, Fridge JL, Bass DM. Enteric parasites, pp: 463-77. In: Wyllie R, Hyams JS, Kay M (eds), Pediatric Gastroenterolgy and Liver Disease. 2016, 5th ed. Philadelphia: Elsevier Inc.
• 25. Usluca S, Aksoy Ü. A Waterborn Parasite: Cryptosporidium. Dokuz Eylül Üniv Tıp Fakültesi Derg 2006; 20(1): 65-74.
• 26. Çiçek M, Körkoca H, Gül A. Investigation of Cryptosporidium sp. in Workers of the Van Municipality Slaughterhouse and in Slaughtered Animals. Türkiye Parazitol Derg 2008; 32(1):8-11.
• 27. Körkoca H, Göz Y, Ataş AD, Kurtoğlu MG, Ekici K, Berktaş M. Prevalence of Cryptosporidium spp. in asymptomatic food workers. Türkiye Parazitol Derg 2013; 37(4): 241-4.
• 28. Ryan U, Fayer R, Xiao L. Cryptosporidium species in humans and animals: current understanding and research needs. Parasitology 2014; 141(13): 1667-85.
• 29. Utaaker KS, Kumar A, Joshi H, Chaudhary S, Robertson LJ. Checking the detail in retail: occurrence of Cryptosporidium and Giardia on vegetables sold across different counters in Chandigarh, India. Int J Food Microbiol 2017; 263:1-8.
• 30. Aydemir S, et al. Investigation of the Effect of Pasteurization on the Viability of Cryptosporidium parvum in Cow's Milk by Propidium Monoazide qPCR. Mikrobiyol Bul 2023; 57(4): 660-666.
• 31. Cryptosporidium, pp: 312-5. In: Kimberlin DW, Brady MT, Jackson MA, Long SS, eds. Red Book: 2015 Report of the Committee on Infectious Diseases. 2015, 30th ed. Elk Grove Village, IL: American Academy of Pediatrics. 32. Karabey M, Can H, Öner TÖ, et al. Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor. Ann Saudı Med 2021; 41(5); 293-298.
• 33. Robertson LJ. Cryptosporidium as a Foodborne Pathogen. 1st ed. New York: Springer; 2014; 1-10.
• 34. Usluca S, Babur C, Kilic S. Current Status in Intestinal Parasitic Infections: A Reference Laboratory Results. KLIMIK Derg 2020; 33(3): 307-314.
• 35. McHardy IH, Wu M, Shimizu-Cohen R, Couturier MR, Humphries RM. Detection of intestinal protozoa in the clinical laboratory. J Clin Microbiol 2014;52(3): 712-20.
• 36. Checkley W, White AJ JR, Jaganath D, Arrowood MJ, Chalmers RM, Chen XM. et al. A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for Cryptosporidium. Lancet Infect Dis 2015;15(1):85- 94.
• 37. Donato B. Tuft Veterinary School scientists decode Cryptosporidium genome. 2004; http:/www./vet. tufts.edu/
• 38. Control and Prevention Diagnostic Procedures for Stool Specimens. 2004. Available from:URL https://www.cdc.gov/dpdx/diagnosticprocedures/index.html
• 39. Roellig DM, Yoder JS, Madison-Antenucci S, Robinson TJ, Van TT, Collier SA. et al. Community laboratory testing for Cryptosporidium: multicenter study retesting public health surveillance stool samples positive for Cryptosporidium by rapid cartridge assay with direct fluorescent antibody testing. PLoS One 2017; 12(1): e0169915
• 40. Yavuz U, Özkan AT. Antigen Detection Methods in Diagnosis of Amebiasis, Giardiasis and Cryptosporiosis. Türkiye Parazitol Derg 2009; 33 (2): 140-150.
• 41. Fayer R. Ungar BL. Cryptosporidium spp. and cryptosporidiosis. Microbiol Rev 1986; 50(4): 458-483
• 42. Barrett TJ. Molecular subtyping for epidemiology: issues in comparability of patterns and interpretation of data In D. H. Persing et al. (ed.), Molecular microbiology, diagnostic, principles and practice. ASM Press, Washington, DC. 2004: 259-268.
• 43. Üner A, Tanrıverdi S, Caner A, Değirmenci A. Cryptosporidium’larda moleküler biyolojik yapı ve çalışmalar. Özcel A, Tanyüksel M, Eren H (eds). Moleküler Parazitoloji Books. 2009; 22: 631-47.
• 44. Köreng B. Entamoeba histolytica/Entamoeba dispar, Giardia lamblia ve Cryptosporidium spp. tanısında mikroskobi, TRIAGE ve ELISA yöntemlerinin karşılaştırılması. Yüksek Lisans Tezi, Çukurova Üniversitesi, Sağlık Bilimleri Enstitüsü, Parazitoloji Anabilim Dalı, 2011.
• 45. Cook WJ, Senkovich O, Hernandez A, Speed H, Chattopadhyay D. Biochemical and structural characterization of Cryptosporidium parvum Lactate dehydrogenase. Int J Biol Macromol 2015; 74: 608-619.
• 46. LaGier MJ, Keithly JS, Zhu G. Characterisation of a novel transporter from Cryptosporidium parvum. Int J Parasitol 2002; 32(7): 877–87.
• 47. Dominguez-Uscanga A, Aycart DF, Li K, Witola WH, Andrade Laborde JE. Anti-protozoal activity of thymol and a thymol ester against Cryptosporidium parvum in cell culture. Int J Parasitol Drugs Drug Resist 2021; 15: 126–33.
• 48. Xiao L, Fayer R, Ryan U, Upton SJ. Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev 2004; 17(1): 72-97.
• 49. Büget E, Büyükbaba-Boral Ö, Kırkoyun-Uysal H, Nazlıcan Ö, Öğüt T, Şengür G. Türkiye’de bir AIDS hastasında ilk mikrosporidiyaz ve solunum sistemini tutan ilk kriptosporidiyaz olgusu. Türk Mikrobiyol Cem Derg 2000; 30(3-4): 166-70.
• 50. Inomata A, Murakoshi F, Ishiwa A, Takano R, Takemae H, Sugi T, et al,. Heparin interacts with elongation factor 1α of Cryptosporidium parvum and inhibits invasion. Sci Rep 2015; 5: 11599.
• 51. Xiao L, Cama VA. Cryptosporidium and Cryptosporidiosis. In: Ortega YR, ed. Foodborne Parasites. Food Microbiology and Food Safety. Newyork, USA: Springer; 2006; 57-86.
• 52. Sudradjat A, Sandhyana Angga M, Barlian K, Nastiti A. Assessing Log Reduction Values of Conventional Water Treatment Plants with Microbially Highly Polluted Raw Water Sources. J Eng Technol Sci 2022; 54(1): 220101.