Hipertermik intraperitoneal kemoterapi uygulanan sıçan modelinde sildenafilin kolon anastomozunu koruyucu etkisi

Year 2025, Volume: 35 Issue: 2, 374 - 381, 30.04.2025

Ahmet Başak , Mehmet Timuçin Aydın , Mehmet Mahir Fersahoğlu , İksan Taşdelen , Adnan Somay , Emre Teke , Erdem Durum , Kemal Memişoğlu

https://doi.org/10.54005/geneltip.1476892

Abstract

Amaç: Çalışmanın amacı hipertermik intraperitoneal kemoterapi uygulanan sıçanlarda kolon anastomozu iyileşmesi üzerine sildenafilin etkilerini araştırmaktır.
Materyal ve Metot: Çalışmamızda 30 adet Wistar-albino cinsi sıçan kullanılmıştır. Sildenafil, kontrol ve sham grubu olmak üzere 3 grupta 10’ar sıçan kullanılmıştır. Tüm gruptaki sıçanlara sol kolon anastomozu yapıldı. Sildenafil grubu ve kontrol grubuna HIPEK işlemi uygulandı. Sildenafil grubuna postoperatif oral sildenafil verildi. 7. gün anastomoz ansı eksize edilerek patlama basıncı ve adezyon değerlendirildi. Anastomoz ansı histopatolojik olarak değerlendirildi. Sonuçlar istatistiksel olarak karşılaştırıldı.
Bulgular: Sonuçlar değerlendirildiğinde patlama basıncı ve adezyon açısından sildenafil grubu ile kontrol grubu arasında fark saptanmamıştır. Sham grubu ise adezyon açısından diğer iki gruba üstün görülmüştür. Histopatolojik incelemede ise sham grubunun anastomoz re-epitelizasyonu ve kas ayrışması açısından diğer iki gruba karşı anlamlı olarak üstün olduğu görülmüştür. Fibrosis ve granülasyon açısından sildenafil grubu ile kontrol grubu arasında anlamlı olarak farklılık saptanmıştır.
Sonuç: HIPEK uygulaması ile beraber yapılan kolon anastomozu üzerinde sildenafilin histopatolojik olarak olumlu etkileri görülmüştür. Kolon anastomozu patlama basınç açısından ise fark görülmemiştir.

Keywords

Hipertermik intraperitoneal kemoterapi, Kolon anastomozu, Anastomoz iyileşmesi, Sildenafil

The protective effect of sildenafil on colon anastomosis in a rat model undergoing hypertermic intraperitoneal chemotherapy

Abstract

Aim: The aim of this study was to investigate the effects of sildenafil on colonic anastomosis healing in rats treated with hyperthermic intraperitoneal chemotherapy.
Method: In our study, 30 Wistar-albino rats were used. 30 rats were divided into 3 groups: the sildenafil, control and sham groups. Left colon anastomosis was performed on rats in all three groups.The HIPEC procedure was applied to sildenafil group and control group. During the postoperative period, sildenafil was given orally to the sildenafil group. On the 7th day, the anastomotic loop was excised, and bursting pressure, and adhesion were evaluated. Anastomotic loop was evaluated histopathologically. The results were compared statistically.
Results: When the results were evaluated considering bursting pressure, and adhesion between the sildenafil group and control group, there was no difference. Considering adhesion, the sham group was superior to the other two groups. Histopathological examination reveals that the sham group was significantly superior to the other two groups in terms of anastomototic re-epitelization and muscle separation. There was a significant difference between the sildenafil group and the control group in terms of fibrosis and granulation.
Conclusion: On colon anastomosis performed together with HIPEC application, histopathologically positive effects of sildenafil were observed. The burst pressure of colon anastomosis was not significantly different.

Keywords

hypertermic intraperitoneal chemotherapy, Colonic anastomosis, Anastomosis healing, Sildenafil

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