CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF MENTHA PIPERITA VAR. CITRATA EXTRACTS OBTAINED BY DIFFERENT EXTRACTION SOLVENTS

Abstract

The main aim of this study was to evaluate the phytochemical composition of extracts of Mentha piperita var. citrata obtained by using three different solvents as well as their antimicrobial activity against a panel of Gram positive and Gram negative bacteria. The antibacterial activities of the extracts (hexane, chloroform, and 70% aqueous methanol) were tested against a panel of bacteria using broth microdilution method. Rosmarinic acid (10.505 mg/g extract), cynaroside (8.545 mg/g extract) and cosmosiin (8.489 mg/g extract) appeared to be the major components of methanolic extract, whereas acacetin was the most abundant (8.438 mg/g extract) component of chloroform extract. M. citrata extracts showed significant antimicrobial activity against Gram positive bacteria at different concentrations. Chloroform extract from M. citrata showed antimicrobial activity at concentration of 512-4096 µg/mL, whereas hexane and methanolic extracts had activity at 1024-4096 µg/mL and 2048-4096 µg/mL, respectively. This study shows the different chemical composition and antimicrobial activities of M. citrata extracts obtained by using different extraction solvents.

Keywords

• Mentha citrata
• antimicrobial activity
• phytochemicals
• methanol extract

MENTHA PIPERITA VAR. CITRATA’NIN FARKLI SOLVENTLER KULLANILARAK HAZIRLARNAN EKSTRAKTLARININ KİMYASAL KOMPOZİSYONLARI VE ANTİMİKROBİYAL AKTİVİTELERİ

Abstract

Bu çalışma farklı çözücüler kullanılarak Mentha piperita var. citrate bitkisinden elde edilen ekstraktların fitokimyasal kompozisyonun belirlenmesi, Gram pozitif ve Gram negatif bakterilere karşı antimikrobiyal etkinliğinin belirlenmesini amaçlamaktadır. Hekzan, kloroform, ve %70’lik metanol ile hazırlanan ekstraktların antimikrobiyal aktiviteleri sıvı mikrodilüsyon metodu kullanılarak test edilmiştir. Rosmarinik asit (10.505 mg/g ekstrakt), sinarosid (8.545 mg/g ekstrakt) ve kosmosiin (8.489 mg/g ekstrakt) metanolik ekstraktda baskın olarak bulunan fitokimyasallar olurken, akasetin (8.438 mg/g ekstrakt) kloroform ekstrakta en fazla bulunan fitokimyasadır. M. citrata ekstraktları Gram pozitif bakterilere karşı farklı konsantrasyonlarda antimikrobiyal aktivite göstermişlerdir. M. citrata bitkisinden elde edilen kloroform ekstraktının antimikrobiyal aktivite aralığı 512-4096 µg/mL iken, hekzan ve metanolik ekstraktlarının aktivitelerinin sırasıyla 1024-4096 µg/mL ve 2048-4096 µg/mL olduğu bulunmuştur. Bu çalışma farklı ekstraksiyon çözücüleri kullanıldığında M. citrata ekstraktlarının farklı kimyasal kompozisyona ve farklı antimikrobiyal aktiviteye sahip olduğunu göstermiştir.

Keywords

• Mentha citrata
• antimikrobiyal aktivite
• fitokimyasallar
• methanol ekstrakt

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